AB282154

Anti-GARS antibody [AT4E10]

Recombinant
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(2 Publications)

Mouse Recombinant Monoclonal GARS antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GARS1 aa 1-300.

View Alternative Names

GARS, GARS1, Glycine--tRNA ligase, Diadenosine tetraphosphate synthetase, Glycyl-tRNA synthetase, Glycyl-tRNA synthetase 1, Ap4A synthetase, GlyRS

2 Images

Supplier Data

Immunocytochemistry - Anti-GARS antibody [AT4E10] (AB282154)

Immunocytochemical analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling GARS with ab282154 at 1/100 dilution followed by Alexa Fluor 488 secondary antibody (green). DAPI was stained the cell nucleus (blue).

Supplier Data

Western blot - Anti-GARS antibody [AT4E10] (AB282154)

SDS-PAGE gel was transferred to PVDF membrane.

ECL detection system.

All lanes:

Western blot - Anti-GARS antibody [AT4E10] (ab282154) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 40 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 40 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 40 µg

Lane 5:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 40 µg

Secondary

All lanes:

Goat anti-Mouse secondary antibody HRP-conjugated

Predicted band size: 83 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AT4E10

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human GARS1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P41250

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 10% Glycerol (glycerin, glycerine), 0.75% Sodium chloride, 0.13% Disodium hydrogenorthophosphate, 0.02% Potassium chloride, 0.02% Potassium phosphate monobasic

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycyl-tRNA synthetase (GARS) also known as GlyRS is an enzyme involved in the protein synthesis process. GARS catalyzes the attachment of glycine to its corresponding transfer RNA (tRNA) enabling the correct integration of glycine into proteins during translation. It has a molecular mass of approximately 79 kDa and is ubiquitously expressed in many tissues including muscle and the nervous system. The enzyme plays a critical function by ensuring the accurate translation of the genetic code into functional proteins.

Biological function summary

GARS maintains the fidelity of protein synthesis ensuring that glycine gets incorporated into proteins correctly. As part of the aminoacyl-tRNA synthetase family it operates within a larger complex important for cellular protein production. GARS ensures that proteins maintain their proper structure and function by providing the correct amino acids during synthesis. Its activity supports cell survival and proper cellular functions across various tissues.

Pathways

The function of GARS is central in aminoacyl-tRNA biosynthesis and the broader protein synthesis pathway. This enzyme works closely with other aminoacyl-tRNA synthetases to ensure the fidelity of protein translation. Interactions with proteins such as EPRS (glutamyl-prolyl-tRNA synthetase) highlight the coordinated effort among different synthetases for efficient protein biosynthesis linking it to a fundamental cellular process essential for cellular viability and function.

Mutations in GARS have been associated with Charcot-Marie-Tooth disease type 2D and distal hereditary motor neuropathy type V. These genetic disorders result in peripheral neuropathy characterized by muscle weakness and sensory deficits. Mutated GARS disrupts normal glycine-tRNA ligation impacting neuronal function. These conditions often involve interactions with proteins like neurofilament proteins contributing to neurodegeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PubMed : 17544401, PubMed : 24898252, PubMed : 28675565). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (PubMed : 19710017).

See full target information GARS1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed40603105

2025

KLF16-Induced Increase of GARS Promotes Glycolysis in Hepatocellular Carcinoma by Stabilizing FOXK1.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Wang,Ruijuan Fan,Jun Li,Hanbo Qiu,Lizhu Lin

Cell communication and signaling : CCS 23:216 PubMed40336063

2025

IKZF1 as a potential therapeutic target for dendritic cell-mediated immunotherapy in IgA nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Peng,Chunjia Sheng,Jiayi He,Yena Zhou,Yilun Qu,Shuwei Duan,Yinghua Zhao,Jikai Xia,Jie Wu,Guangyan Cai,Lingling Wu,Chuyue Zhang,Xiangmei Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GARS antibody [AT4E10]

Immunocytochemistry - Anti-GARS antibody [AT4E10] (AB282154)

Western blot - Anti-GARS antibody [AT4E10] (AB282154)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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