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AB187716

Anti-GART antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal GART antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GART aa 200-250.

View Alternative Names

PGFT, PRGS, GART, Trifunctional purine biosynthetic protein adenosine-3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody (AB187716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody (AB187716)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling GART with ab187716 at a 1/1000 dilution.

Immunoprecipitation - Anti-GART antibody (AB187716)
  • IP

Supplier Data

Immunoprecipitation - Anti-GART antibody (AB187716)

Immunoprecipitation analysis of 293T whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) immunoprecipitating GART using ab187716 at 6 μg per reaction. (Lane 1).

GART was also immunoprecipitated using an other antibody anti GART (Lane 2). Lane 3 is Control IgG.

All lanes:

Immunoprecipitation - Anti-GART antibody (ab187716)

Predicted band size: 107 kDa

true

Exposure time: 3min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody (AB187716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GART antibody (AB187716)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse teratoma tissue labeling GART with ab187716 at a 1/1000 dilution.

Western blot - Anti-GART antibody (AB187716)
  • WB

Supplier Data

Western blot - Anti-GART antibody (AB187716)

All lanes:

Western blot - Anti-GART antibody (ab187716) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

293T cell lysate at 50 µg

Lane 3:

Jurkat cell lysate at 50 µg

Lane 4:

Mouse TCMK-1 cell lysate at 50 µg

Lane 5:

Mouse NIH3T3 cell lysate at 50 µg

Predicted band size: 107 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human GART aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P22102

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GART also known as phosphoribosylglycinamide formyltransferase is a multifunctional enzyme with a mass of approximately 100 kDa. It plays a significant mechanical role in purine biosynthesis. GART is primarily expressed in rapidly dividing cells and tissues with high metabolic activity. It is one of three enzymes encoded by the GART gene contributing to the trifunctional protein complex that includes glycinamide ribonucleotide synthetase (GARS) and aminoimidazole ribonucleotide synthetase (AIRS). These enzyme functions are critical for the mechanistic steps in purine metabolism increasing their importance in synthesizing nucleotides for DNA and RNA production.
Biological function summary

GART is involved in synthesizing purine nucleotides through its role in converting phosphoribosylamine to phosphoribosylglycinamide. It does this by catalyzing the transfer of a formyl group highlighting its operation as part of a trifunctional protein. The complex formation with enzymes GARS and AIRS enables efficient handling of intermediates therefore fostering a streamlined biosynthetic process. This contributes directly to the cellular nucleotide pool which is fundamental for cell proliferation signaling and energy transfer.

Pathways

GART fits into the purine biosynthetic pathway which is essential for nucleotide synthesis. This function links it closely with proteins such as IMP dehydrogenase which further processes inosine monophosphate to produce different purine nucleotides. It is also involved in folate metabolism as it requires folate derivatives for its formyl transferase activity. The close association with these pathways illustrates GART's integral role in both cellular growth and maintenance of genetic material.

GART has implications in cancer and neurological disorders due to its role in nucleotide synthesis and cellular proliferation. Elevated expression levels of GART are observed in tumors indicating its potential involvement in oncogenesis. In addition abnormalities in purine metabolism or folate cycling can contribute to disorders like Down syndrome. Within this context proteins interacting with GART such as GARS and AIRS could potentially serve as associated targets for therapeutic intervention emphasizing the enzyme's relevance in disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Trifunctional enzyme that catalyzes three distinct reactions as part of the 'de novo' inosine monophosphate biosynthetic pathway.
See full target information GART
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 14:1129162 PubMed37143728

2023

A functional genetic screen for metabolic proteins unveils GART and the purine biosynthetic pathway as novel targets for the treatment of luminal A ERα expressing primary and metastatic invasive ductal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Cipolletti,Stefano Leone,Stefania Bartoloni,Filippo Acconcia

RNA biology 17:1811-1822 PubMed32658600

2020

Circular RNA circ-LDLRAD3 serves as an oncogene to promote non-small cell lung cancer progression by upregulating SLC1A5 through sponging miR-137.

Applications

Unspecified application

Species

Unspecified reactive species

Min Xue,Weijun Hong,Jun Jiang,Fang Zhao,Xiwen Gao
View all publications
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Product promise

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