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Rabbit Recombinant Monoclonal GAS 6 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-GAS 6 antibody [EPR23931-27] (AB264098), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-GAS 6 antibody [EPR23931-27] (AB264098), expandable thumbnail
  • Immunoprecipitation - Anti-GAS 6 antibody [EPR23931-27] (AB264098), expandable thumbnail
  • Western blot - Anti-GAS 6 antibody [EPR23931-27] (AB264098), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-GAS 6 antibody [EPR23931-27] (AB264098), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. (Microbial infection) Can bridge virus envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry by apoptotic mimicry (PubMed:21501828). Plays a role in Dengue cell entry by apoptotic mimicry (PubMed:23084921). Plays a role in Vaccinia virus cell entry by apoptotic mimicry (PubMed:21501828). Plays a role in ebolavirus and marburgvirus cell entry by apoptotic mimicry (PubMed:17005688).

Alternative names

AXLLG, GAS6, Growth arrest-specific protein 6, GAS-6, AXL receptor tyrosine kinase ligand

Recommended products

Rabbit Recombinant Monoclonal GAS 6 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23931-27
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein Gas6 also known as growth arrest-specific 6 weighs approximately 75 kDa and functions mechanically as a ligand for the TAM (Tyro3 Axl and Mer) receptor tyrosine kinases. Gas6 protein facilitates cell signaling by binding especially to AXL receptor initiating various downstream cellular responses. It is widely expressed in different tissues including the nervous system cardiovascular system and kidneys. Additionally its expression increases in response to cellular stress and inflammatory stimuli.

Biological function summary

Gas6 influences several cellular processes such as cell survival proliferation and migration. Gas6 exerts its effects in a complex with AXL receptor which is part of a larger network involving other TAM receptors. By activating AXL Gas6 modulates immune responses inflammation and tissue repair. The protein also plays a significant role in phagocytosis helping remove apoptotic cells thereby maintaining tissue homeostasis.

Pathways

The Gas6 protein is significantly engaged in the PI3K/AKT signaling pathway important for promoting cell survival and inhibiting apoptosis. Additionally Gas6 interacts with the NF-kB pathway which regulates inflammation and immune responses. Both pathways highlight the synergy between Gas6 and AXL emphasizing their contribution to cellular integrity and response to injury.

Associated diseases and disorders

Gas6's dysregulation associates with cancer and atherosclerosis. In oncogenesis altered Gas6-AXL signaling promotes tumor progression and resistance to therapy implicating it in diverse cancers. Meanwhile in cardiovascular diseases elevated Gas6 levels correlate with atherosclerosis interacting with inflammatory proteins such as interleukins. Understanding Gas6's role in these conditions offers potential for therapeutic targeting and biomarker development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-GAS 6 antibody [EPR23931-27] (ab264098), expandable thumbnail

    Western blot - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

    False colour image of Western blot: Anti-GAS 6 antibody [EPR23931-27] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab264098 was shown to bind specifically to GAS 6. A band was observed at 75 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in GAS6 knockout cell line Human GAS6 knockout HEK-293 cell line ab275837 (knockout cell lysate Human GAS6 knockout HEK-293 cell lysate ab275811). To generate this image, wild-type and GAS6 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-GAS 6 antibody [EPR23931-27] (ab264098) at 1/1000 dilution

    Lane 1: Wild-type HEK-293 cell lysate at 20 µg

    Lane 2: GAS6 knockout HEK-293 cell lysate at 20 µg

    Lane 2: Western blot - Human GAS6 knockout HEK-293 cell line (Human GAS6 knockout HEK-293 cell line ab275837)

    Lane 3: THP-1 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 80 kDa

    Observed band size: 75 kDa

  • Flow Cytometry (Intracellular) - Anti-GAS 6 antibody [EPR23931-27] (ab264098), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling GAS 6 with ab264098 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab264098.

  • Immunoprecipitation - Anti-GAS 6 antibody [EPR23931-27] (ab264098), expandable thumbnail

    Immunoprecipitation - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

    GAS 6 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab264098 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264098 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate 10 ug

    Lane 2: ab264098 IP in THP-1 whole cell lysate

    Lane 3:Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab264098 in THP-1 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

    All lanes: Immunoprecipitation - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

    Predicted band size: 80 kDa

    Observed band size: 78 kDa

  • Western blot - Anti-GAS 6 antibody [EPR23931-27] (ab264098), expandable thumbnail

    Western blot - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: Jurkat (PMID:10400186)

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: Lanes 1-2: 48 seconds;Lanes 3-4: 3 minutes.

    All lanes: Western blot - Anti-GAS 6 antibody [EPR23931-27] (ab264098) at 1/1000 dilution

    Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: Jurkat (human t cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 80 kDa

    Observed band size: 78 kDa

  • Flow Cytometry (Intracellular) - Anti-GAS 6 antibody [EPR23931-27] (ab264098), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling GAS 6 with ab264098 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab264098. Cells were co-stained with DRAQ/ Ki-67 to differentiate cell cycle phase.

  • Flow Cytometry (Intracellular) - Anti-GAS 6 antibody [EPR23931-27] (ab264098), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (Human T cell leukemia T lymphocyte (Left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labelling GAS 6 with ab264098 at 1/500 dilution (Right). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID:10400186)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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