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AB236618

Anti-Gas1 antibody

5

(1 Review)

|

(5 Publications)

Rabbit Polyclonal GAS1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Growth arrest-specific protein 1 aa 200 to C-terminus.

View Alternative Names

Growth arrest-specific protein 1, GAS-1, GAS1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gas1 antibody (AB236618)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gas1 antibody (AB236618)

Paraffin-embedded human skin tissue stained for Gas1 with ab236618 at a 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gas1 antibody (AB236618)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gas1 antibody (AB236618)

Paraffin-embedded human pancreas tissue stained for Gas1 with ab236618 at a 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Gas1 antibody (AB236618)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Gas1 antibody (AB236618)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Gas1 (Green) using ab236618 at a 1/133 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Western blot - Anti-Gas1 antibody (AB236618)
  • WB

Supplier Data

Western blot - Anti-Gas1 antibody (AB236618)

All lanes:

Western blot - Anti-Gas1 antibody (ab236618) at 1/500 dilution

Lane 1:

SH-SH5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Growth arrest-specific protein 1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54826

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The growth arrest-specific 1 protein or Gas1 plays an inhibitory role in cell cycle progression by interacting with various growth factors and receptors. Gas1 often termed as Gas-1 is a 38 kDa protein. It expresses prominently in quiescent cells particularly in the nervous and embryonic tissues. This expression helps control the proliferation of cells which is critical for maintaining normal cellular functions and structure.
Biological function summary

Gas1 acts as a negative regulator of cell proliferation particularly during developmental processes. It is not part of a complex but serves as a standalone inhibitor that influences cell growth. The protein interacts with components like Hedgehog signaling which leads to its involvement in developmental signaling pathways. Gas1's role in growth arrest demonstrates its importance in controlling the balance of cellular differentiation and proliferation.

Pathways

Gas1 significantly influences the Hedgehog signaling pathway and the G1 phase of the cell cycle. In the Hedgehog pathway Gas1 interacts with other key proteins like Patched and Smoothened regulating the pathway's activity which is essential for embryonic development and tissue patterning. Its interaction with these proteins highlights its regulatory function in signal transduction influencing how cells respond to growth cues.

Gas1's dysregulation links to cancer and congenital malformations. Such disorders often occur due to alterations in the Hedgehog signaling pathway implicating Gas1 as a contributor in aberrant cell growth conditions. In cancer specifically Gas1 may influence tumor suppression mechanisms impacting cells' growth arrest. Mutations in components of the Hedgehog pathway such as Smoothened can alter Gas1's function giving rise to developmental disorders and cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells. Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).
See full target information Growth arrest-specific protein 1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:116025 PubMed40700020

2025

IGSF10 is a RET antagonist regulating Ewing sarcoma growth and GnRH neuron migration.

Applications

Unspecified application

Species

Unspecified reactive species

Panneerselvam Jayabal,Xiuye Ma,Maleeha Akram,Hannah Bow,Pranay Mandal,Naira Mansano,Yidong Chen,Susan T Weintraub,Peter J Houghton,Susan Wray,Yuzuru Shiio

Stem cell research & therapy 12:150 PubMed33632317

2021

Melatonin promotes bone marrow mesenchymal stem cell osteogenic differentiation and prevents osteoporosis development through modulating circ_0003865 that sponges miR-3653-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Xudong Wang,Taiqiu Chen,Zhihuai Deng,Wenjie Gao,Tongzhou Liang,Xianjian Qiu,Bo Gao,Zizhao Wu,Jincheng Qiu,Yuanxin Zhu,Yanbo Chen,Zhancheng Liang,Hang Zhou,Caixia Xu,Anjing Liang,Peiqiang Su,Yan Peng,Dongsheng Huang

Journal of experimental & clinical cancer research 40:44 PubMed33499877

2021

A positive feedback loop between TAZ and miR-942-3p modulates proliferation, angiogenesis, epithelial-mesenchymal transition process, glycometabolism and ROS homeostasis in human bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feifan Wang,Mengjing Fan,Xuejian Zhou,Yanlan Yu,Yueshu Cai,Hongshen Wu,Yan Zhang,Jiaxin Liu,Shihan Huang,Ning He,Zhenghui Hu,Guoqing Ding,Xiaodong Jin

Cancer management and research 12:3401-3407 PubMed32494204

2020

LncRNA LUADT1 Promotes Oral Squamous Cell Carcinoma Cell Proliferation by Regulating miR-34a/GAS1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lirong Gao,Siming Wang,Junru Meng,Yugang Sun

Developmental cell 47:439-452.e6 PubMed30318244

2018

A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Bipul R Acharya,Alexander Nestor-Bergmann,Xuan Liang,Shafali Gupta,Kinga Duszyc,Estelle Gauquelin,Guillermo A Gomez,Srikanth Budnar,Philippe Marcq,Oliver E Jensen,Zev Bryant,Alpha S Yap
View all publications
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Product promise

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