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AB242777

Anti-Gas1 antibody [EPR17987-16] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal GAS1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Gas-1, Gas1, Growth arrest-specific protein 1, GAS-1

1 Images
Sandwich ELISA - Anti-Gas1 antibody [EPR17987-16] - BSA and Azide free (Detector) (AB242777)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Gas1 antibody [EPR17987-16] - BSA and Azide free (Detector) (AB242777)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab205573).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17987-16

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab242777 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Gas1. The recommended pair for sandwich ELISA is:
Capture: ab242530, Mouse Gas1 Capture Antibody (unconjugated)
Detector: ab242777, Mouse Gas1 Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The growth arrest-specific 1 protein or Gas1 plays an inhibitory role in cell cycle progression by interacting with various growth factors and receptors. Gas1 often termed as Gas-1 is a 38 kDa protein. It expresses prominently in quiescent cells particularly in the nervous and embryonic tissues. This expression helps control the proliferation of cells which is critical for maintaining normal cellular functions and structure.
Biological function summary

Gas1 acts as a negative regulator of cell proliferation particularly during developmental processes. It is not part of a complex but serves as a standalone inhibitor that influences cell growth. The protein interacts with components like Hedgehog signaling which leads to its involvement in developmental signaling pathways. Gas1's role in growth arrest demonstrates its importance in controlling the balance of cellular differentiation and proliferation.

Pathways

Gas1 significantly influences the Hedgehog signaling pathway and the G1 phase of the cell cycle. In the Hedgehog pathway Gas1 interacts with other key proteins like Patched and Smoothened regulating the pathway's activity which is essential for embryonic development and tissue patterning. Its interaction with these proteins highlights its regulatory function in signal transduction influencing how cells respond to growth cues.

Gas1's dysregulation links to cancer and congenital malformations. Such disorders often occur due to alterations in the Hedgehog signaling pathway implicating Gas1 as a contributor in aberrant cell growth conditions. In cancer specifically Gas1 may influence tumor suppression mechanisms impacting cells' growth arrest. Mutations in components of the Hedgehog pathway such as Smoothened can alter Gas1's function giving rise to developmental disorders and cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells. Binds 20(S)-hydroxycholesterol (20(S)-OHC) (PubMed : 34799735).
See full target information Gas1
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Product promise

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For full details, please see our Terms & Conditions

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