AB205018

Anti-GAS41 antibody

Name: Anti-GAS41 antibody (ab205018) | Abcam
Brand: Abcam Limited
SKU: AB205018
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal GAS41 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human YEATS4 aa 150 to C-terminus.

View Alternative Names

GAS41, YEATS4, YEATS domain-containing protein 4, Glioma-amplified sequence 41, NuMA-binding protein 1, Gas41, NuBI-1, NuBI1

2 Images

Supplier Data

Immunoprecipitation - Anti-GAS41 antibody (AB205018)

Detection of GAS41 in Immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab205018 at 6 μg/mg lysate for IP (Lane

and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-GAS41 antibody (ab205018)

Predicted band size: 26 kDa

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Supplier Data

Western blot - Anti-GAS41 antibody (AB205018)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-GAS41 antibody (ab205018) at 0.1 µg/mL

Lane 1:

Hela whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 26 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human YEATS4 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95619

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GAS41 also known as Glioma-Amplified Sequence 41 weighs approximately 26 kDa. It is ubiquitously expressed in various cell types including brain and neuronal tissues. GAS41 performs as a component of the chromatin remodeling complex. This protein participates in DNA binding and potentially regulates transcription processes influencing gene expression patterns.

Biological function summary

GAS41 impacts cellular proliferation and organization. It is often part of the Tip60/NuA4 histone acetyltransferase complex which is involved in chromatin modification. Through its role in this complex GAS41 influences transcriptional regulation by modifying chromatin architecture. This process ultimately affects how genes are turned on or off in cells which is key for maintaining cellular function and identity.

Pathways

GAS41 plays a significant role in the regulation of gene expression pathways and cell cycle control. It interacts with proteins in the SWI/SNF-related chromatin remodeler pathways and modulates how genes involved in cell cycle progression are expressed. GAS41 shows interaction with proteins like p400 which also contributes to these pathways indicating its importance in cellular growth and division.

GAS41 is linked to glioma progression and certain neurodegenerative disorders. Changes in the expression of GAS41 have been observed in gliomas connecting it with the uncontrolled cell growth characteristic of these tumors. Through its association with other proteins like ATRX GAS41 is implicated in chromatin remodeling disturbances seen in neurodegenerative diseases influencing gene expression patterns and contributing to disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chromatin reader component of the NuA4 histone acetyltransferase (HAT) complex, a complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed : 12963728, PubMed : 14966270). Specifically recognizes and binds acylated histone H3, with a preference for histone H3 diacetylated at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac) or histone H3 diacetylated at 'Lys-14' and 'Lys-27' (H3K14ac and H3K27ac) (PubMed : 29437725, PubMed : 29900004, PubMed : 30071723). Also able to recognize and bind crotonylated histone H3 (PubMed : 30071723). May also recognize and bind histone H3 succinylated at 'Lys-122' (H3K122succ); additional evidences are however required to confirm this result in vivo (PubMed : 29463709). Plays a key role in histone variant H2AZ1/H2A.Z deposition into specific chromatin regions : recognizes and binds H3K14ac and H3K27ac on the promoters of actively transcribed genes and recruits NuA4-related complex to deposit H2AZ1/H2A.Z (PubMed : 29437725). H2AZ1/H2A.Z deposition is required for maintenance of embryonic stem cell (By similarity).

See full target information YEATS4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 622:173-179 PubMed37731000

2023

Acetyl-methyllysine marks chromatin at active transcription start sites.

Applications

Unspecified application

Species

Unspecified reactive species

William J Lu-Culligan,Leah J Connor,Yixuan Xie,Babatunde E Ekundayo,Brendan T Rose,Martin Machyna,Andreas P Pintado-Urbanc,Joshua T Zimmer,Isaac W Vock,Natarajan V Bhanu,Megan C King,Benjamin A Garcia,Franziska Bleichert,Matthew D Simon

Scientific reports 10:6787 PubMed32321993

2020

A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvie Thuault,Claire Mamelonet,Joëlle Salameh,Kevin Ostacolo,Brice Chanez,Danièle Salaün,Emilie Baudelet,Stéphane Audebert,Luc Camoin,Ali Badache

Oncology research 25:1633-1641 PubMed28251887

2017

YEATS Domain Containing 4 Promotes Gastric Cancer Cell Proliferation and Mediates Tumor Progression via Activating the Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sheqing Ji,Youxiang Zhang,Binhai Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GAS41 antibody

Immunoprecipitation - Anti-GAS41 antibody (AB205018)

Western blot - Anti-GAS41 antibody (AB205018)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise