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AB236386

Anti-GAS8 antibody [OTI10G2]

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(1 Publication)

Mouse Monoclonal GAS8 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GAS8.

View Alternative Names

DRC4, GAS11, GAS8, Dynein regulatory complex subunit 4, Growth arrest-specific protein 11, Growth arrest-specific protein 8, GAS-11, GAS-8

1 Images
Western blot - Anti-GAS8 antibody [OTI10G2] (AB236386)
  • WB

Supplier Data

Western blot - Anti-GAS8 antibody [OTI10G2] (AB236386)

All lanes:

Western blot - Anti-GAS8 antibody [OTI10G2] (ab236386) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY GAS8 cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Predicted band size: 56 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI10G2

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GAS8.

O95995

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Clone OTI10G2 (formerly 10G2).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as Growth arrest-specific protein 8 (GAS8) which is also referred to as GAS11 plays an important role in the mechanical stability and movement of intracellular structures. It has an approximate mass of 87 kDa. GAS8 is expressed in a range of tissues but shows high expression in ciliated structures such as in the respiratory tract and reproductive tissues. It operates mainly as a component of the microtubule organizing centers associating with various cytoskeletal elements.
Biological function summary

Within the framework of cell function GAS8 interacts with the axonemal dynein complex. This protein aids in regulating ciliary motion essential for proper cilia and flagella function. As a component of the axoneme a structural feature of cilia and flagella GAS8 contributes to microtubule network stability and arrangement. It also bridges interactions between microtubules and ciliary dynein thereby facilitating efficient movement.

Pathways

GAS8 exerts its functions predominantly in the dynein arm assembly pathway. This pathway is critical for axonemal dynein arm stabilization important for beating motion. GAS8 works in concert with proteins like dynein light chain and axonemal dynein heavy chain to form functional ciliary complexes. These pathways are vital for cell signaling and fluid movement across epithelial cells.

Impaired GAS8 function has connections to primary ciliary dyskinesia (PCD) and respiratory disorders. Abnormalities in GAS8 expression or mutation can result in defective cilia leading to respiratory illnesses and symptoms of PCD such as chronic bronchitis and sinusitis. The interaction between GAS8 and other axonemal proteins like dynein helps form a comprehensive understanding of their impact on hereditary ciliopathies and disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Plays an important role in the assembly of the N-DRC linker (By similarity). Plays dual roles at both the primary (or non-motile) cilia to regulate hedgehog signaling and in motile cilia to coordinate cilia movement. Required for proper motile cilia functioning (PubMed : 26387594, PubMed : 27120127, PubMed : 27472056). Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity in a GRK2-dependent manner (By similarity).
See full target information GAS8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Dose-response : a publication of International Hormesis Society 17:1559325819886766 PubMed31762715

2019

mRNA and lncRNA Expression Profiling of Radiation-Induced Gastric Injury Reveals Potential Radiation-Responsive Transcription Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Guangxia Chen,Yang Feng,Zhiqiang Sun,Yiying Gao,Chuannan Wu,Haihan Zhang,Jinming Cao,Zhuo Chen,Jianping Cao,Yaqun Zhu,Shuyu Zhang
View all publications
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