AB192318

Anti-Gastric Mucin/MUC-6 antibody

Name: Anti-Gastric Mucin/MUC-6 antibody (ab192318) | Abcam
Brand: Abcam Limited
SKU: AB192318

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(6 Publications)

Rabbit Polyclonal Gastric Mucin/MUC-6 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MUC6.

View Alternative Names

Mucin-6, MUC-6, Gastric mucin-6, MUC6

1 Images

Supplier Data

Western blot - Anti-Gastric Mucin/MUC-6 antibody (AB192318)

All lanes:

Western blot - Anti-Gastric Mucin/MUC-6 antibody (ab192318)

Lane 1:

HEK293T whole cell lysate

Lane 2:

RAW 264.7 whole cell lysate

Lane 3:

Rat kidney tissue lysate

Predicted band size: 257 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human MUC6. The exact immunogen used to generate this antibody is proprietary information.

Q6W4X9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab192318 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage buffer

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MUC-6 also called gastric mucin is a large glycoprotein with a molecular mass around 500-1000 kDa. Cells in the stomach express MUC-6 where it plays a critical mechanical role in forming protective mucous barriers against harsh gastric environments. The glycoprotein forms viscous gels that coat the gastric mucosa creating a protective layer. These layers prevent autodigestion by gastric acid and pepsin safeguarding the underlying epithelium from damage.

Biological function summary

MUC-6 contributes to the defense of gastric tissues through its complex structure. The protein belongs to the mucin family characterized by their high carbohydrate content. MUC-6 interacts with other secreted mucins like MUC-5AC to form a protective gel. It acts as an important component in the maintenance of the gastric mucosal barrier ensuring the separation of stomach contents and epithelial surface. This gel traps pathogens and particles reducing their ability to cause infection or irritation.

Pathways

MUC-6 plays a role in mucosal immunity and epithelial signaling pathways. In the gastric mucosa pathway it links with proteins such as CLCA1 which influences mucin secretion and function. Another linked pathway is the oxidative stress pathway where MUC-6 partners with antioxidant enzymes to shield the stomach lining from oxidative damage. These pathways showcase how MUC-6 integrates a mechanical and biological defense system within the digestive tract.

Alterations in MUC-6 expression levels can influence conditions like gastric cancer and peptic ulcer disease. In gastric cancer abnormal methylation of the MUC-6 gene can contribute to tumorigenesis and tumor progression. In peptic ulcer disease deficient mucin levels particularly MUC-6 compromise the mucosal barrier function. Connections also exist with proteins like gastrin which elevate acid production and burden the mucosal defense emphasizing the relevance of MUC-6 in these gastric conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Plays an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.

See full target information MUC6

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular cell biology 16: PubMed39153963

2024

Modeling gastric intestinal metaplasia in 3D organoids using nitrosoguanidine.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Li,Jiena Chen,Tao Li,Jie Lin,Haocheng Zheng,Nadia Johnson,Xuebiao Yao,Xia Ding

Science advances 9:eadh0322 PubMed37967175

2023

Inactivation of the tumor suppressor gene synergizes with to induce DNA damage in murine gastric stem and progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiazhuo He,Zuzana Nascakova,Peter Leary,Giovanni Papa,Tomas Valenta,Konrad Basler,Anne Müller

Frontiers in oncology 12:756117 PubMed35574418

2022

Tumor Suppressive Role of MUC6 in Wilms Tumor Autophagy-Dependent β-Catenin Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Bai-Hui Liu,Gong-Bao Liu,Bin-Bin Zhang,Jian Shen,Lu-Lu Xie,Xiang-Qi Liu,Wei Yao,Rui Dong,Yun-Li Bi,Kui-Ran Dong

International journal of molecular medicine 47: PubMed33907823

2021

IRF1 regulates the progression of colorectal cancer via interferon‑induced proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Xu,Yong Wu,Ke Yi,Yan Hu,Weiqun Ding,Chungen Xing

Cell research 30:1078-1087 PubMed33159154

2020

Mucus production stimulated by IFN-AhR signaling triggers hypoxia of COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Yuying Liu,Jiadi Lv,Jiangning Liu,Man Li,Jing Xie,Qi Lv,Wei Deng,Nannan Zhou,Yabo Zhou,Jiangping Song,Peng Wang,Chuan Qin,Wei-Min Tong,Bo Huang

International journal of oncology 56:494-507 PubMed31894271

2020

Long non‑coding RNA CASC2 suppresses pancreatic cancer cell growth and progression by regulating the miR‑24/MUC6 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Da-Fang Xu,Li-Shan Wang,Jia-Hua Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Gastric Mucin/MUC-6 antibody

Western blot - Anti-Gastric Mucin/MUC-6 antibody (AB192318)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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