Mouse Monoclonal Gastric Mucin/MUC-6 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MUC6.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [CLH5] - BSA and Azide free (AB212646), expandable thumbnail](https://content.abcam.com/products/images/gastric-mucin-muc-6-antibody-clh5-bsa-and-azide-free-ab212646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62863.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [CLH5] - BSA and Azide free (AB212646)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human MUC6. The exact immunogen used to generate this antibody is proprietary information. Database link Q6W4X9
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg/mL | Notes (Primary incubation for 30 minutes at RT). Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Plays an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.
Alternative names
Mucin-6, MUC-6, Gastric mucin-6, MUC6
Recommended products
Mouse Monoclonal Gastric Mucin/MUC-6 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MUC6.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human MUC6. The exact immunogen used to generate this antibody is proprietary information. Database link Q6W4X9
- Carrier free
- Yes
- Clone number
- CLH5
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
- Purification notes
ab212646 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MUC-6 also called gastric mucin is a large glycoprotein with a molecular mass around 500-1000 kDa. Cells in the stomach express MUC-6 where it plays a critical mechanical role in forming protective mucous barriers against harsh gastric environments. The glycoprotein forms viscous gels that coat the gastric mucosa creating a protective layer. These layers prevent autodigestion by gastric acid and pepsin safeguarding the underlying epithelium from damage.
- Biological function summary
MUC-6 contributes to the defense of gastric tissues through its complex structure. The protein belongs to the mucin family characterized by their high carbohydrate content. MUC-6 interacts with other secreted mucins like MUC-5AC to form a protective gel. It acts as an important component in the maintenance of the gastric mucosal barrier ensuring the separation of stomach contents and epithelial surface. This gel traps pathogens and particles reducing their ability to cause infection or irritation.
- Pathways
MUC-6 plays a role in mucosal immunity and epithelial signaling pathways. In the gastric mucosa pathway it links with proteins such as CLCA1 which influences mucin secretion and function. Another linked pathway is the oxidative stress pathway where MUC-6 partners with antioxidant enzymes to shield the stomach lining from oxidative damage. These pathways showcase how MUC-6 integrates a mechanical and biological defense system within the digestive tract.
- Associated diseases and disorders
Alterations in MUC-6 expression levels can influence conditions like gastric cancer and peptic ulcer disease. In gastric cancer abnormal methylation of the MUC-6 gene can contribute to tumorigenesis and tumor progression. In peptic ulcer disease deficient mucin levels particularly MUC-6 compromise the mucosal barrier function. Connections also exist with proteins like gastrin which elevate acid production and burden the mucosal defense emphasizing the relevance of MUC-6 in these gastric conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [CLH5] - BSA and Azide free (ab212646), expandable thumbnail](https://content.abcam.com/products/images/gastric-mucin-muc-6-antibody-clh5-bsa-and-azide-free-ab212646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62863.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [CLH5] - BSA and Azide free (ab212646)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [CLH5] - BSA and Azide free (ab212646)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue labeling Gastric Mucin/MUC-6 with ab212646 at 4 μg/ml dilution.
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