Skip to main content

Rabbit Recombinant Monoclonal Gastric Mucin/MUC-6 antibody. Suitable for mIHC, IHC-P and reacts with Human samples. Cited in 1 publication.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (AB223846), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (AB223846), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (AB223846), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (AB223846), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (AB223846), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Plays an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.

Alternative names

Mucin-6, MUC-6, Gastric mucin-6, MUC6

Recommended products

Rabbit Recombinant Monoclonal Gastric Mucin/MUC-6 antibody. Suitable for mIHC, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20623

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MUC-6 also called gastric mucin is a large glycoprotein with a molecular mass around 500-1000 kDa. Cells in the stomach express MUC-6 where it plays a critical mechanical role in forming protective mucous barriers against harsh gastric environments. The glycoprotein forms viscous gels that coat the gastric mucosa creating a protective layer. These layers prevent autodigestion by gastric acid and pepsin safeguarding the underlying epithelium from damage.

Biological function summary

MUC-6 contributes to the defense of gastric tissues through its complex structure. The protein belongs to the mucin family characterized by their high carbohydrate content. MUC-6 interacts with other secreted mucins like MUC-5AC to form a protective gel. It acts as an important component in the maintenance of the gastric mucosal barrier ensuring the separation of stomach contents and epithelial surface. This gel traps pathogens and particles reducing their ability to cause infection or irritation.

Pathways

MUC-6 plays a role in mucosal immunity and epithelial signaling pathways. In the gastric mucosa pathway it links with proteins such as CLCA1 which influences mucin secretion and function. Another linked pathway is the oxidative stress pathway where MUC-6 partners with antioxidant enzymes to shield the stomach lining from oxidative damage. These pathways showcase how MUC-6 integrates a mechanical and biological defense system within the digestive tract.

Associated diseases and disorders

Alterations in MUC-6 expression levels can influence conditions like gastric cancer and peptic ulcer disease. In gastric cancer abnormal methylation of the MUC-6 gene can contribute to tumorigenesis and tumor progression. In peptic ulcer disease deficient mucin levels particularly MUC-6 compromise the mucosal barrier function. Connections also exist with proteins like gastrin which elevate acid production and burden the mucosal defense emphasizing the relevance of MUC-6 in these gastric conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (ab223846), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (ab223846)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Gastric Mucin/MUC-6 with ab223846 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human stomach (PMID:17227128). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (ab223846), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (ab223846)

    Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling Gastric Mucin/MUC-6 with ab223846 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Brunner's glands of human duodenum (PMID:10681391). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (ab223846), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (ab223846)

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Gastric Mucin/MUC-6 with ab223846 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human gastric cancer (PMID: 14557879). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Multiplex immunohistochemistry - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (ab223846), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (ab223846)

    Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Chromogranin A (Anti-Chromogranin A antibody [EPR22537-249] ab254557, gray; Opal™690), anti-MUC-6 (ab223846, green; Opal™520) and anti-Rab3D (Anti-Rab3D antibody [EPR8106] ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Chromogranin A stained on neuroendocrine cells. Panel D: anti-Rab3D stained on Chief cells. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-Chromogranin A antibody [EPR22537-249] ab254557 (1/5000), ab223846 (1/1000), and Anti-Rab3D antibody [EPR8106] ab128997 (1/10000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (ab223846), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (ab223846)

    Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Mucin 5AC (Anti-Mucin 5AC antibody [45M1] ab3649, gray; Opal™690), anti-MUC-6 (ab223846, green; Opal™520) and anti-Rab3D (Anti-Rab3D antibody [EPR8106] ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Mucin 5AC stained on surface mucous cells. Panel D: anti-Rab3D stained on Chief cells. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-Mucin 5AC antibody [45M1] ab3649 (1/5000 dilution), ab223846 (1/1000 dilution), and Anti-Rab3D antibody [EPR8106] ab128997 (1/10000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com