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AB232775

Anti-Gastrin antibody

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(4 Publications)

Rabbit Polyclonal Gastrin antibody. Suitable for WB, IHC-P and reacts with Rat, Pig, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GAST.

View Alternative Names

GAS, GAST, Gastrin

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody (AB232775)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody (AB232775)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Gastrin using ab232775 at 20 μg/ml in immunohistochemical analysis.

Western blot - Anti-Gastrin antibody (AB232775)
  • WB

Supplier Data

Western blot - Anti-Gastrin antibody (AB232775)

All lanes:

Western blot - Anti-Gastrin antibody (ab232775) at 2 µg/mL

All lanes:

Pig stomach lysate

Predicted band size: 11 kDa

false

Western blot - Anti-Gastrin antibody (AB232775)
  • WB

Supplier Data

Western blot - Anti-Gastrin antibody (AB232775)

All lanes:

Western blot - Anti-Gastrin antibody (ab232775) at 2 µg/mL

All lanes:

Recombinant human Gastrin protein

Predicted band size: 11 kDa,165 kDa,36 kDa,47 kDa

false

Western blot - Anti-Gastrin antibody (AB232775)
  • WB

Supplier Data

Western blot - Anti-Gastrin antibody (AB232775)

All lanes:

Western blot - Anti-Gastrin antibody (ab232775) at 2 µg/mL

All lanes:

Rat stomach lysate

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GAST.

P01350

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab232775 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gastrin sometimes referred to as gastrin peptide is a hormone consisting of 34 amino acids with a molecular mass of approximately 3.8 kDa. This protein is mainly expressed in the G cells of the stomach lining specifically the antrum but also found in the duodenum and pancreas. Gastrin operates by stimulating the secretion of gastric acid from parietal cells in the stomach playing an important role in the digestive process. The presence of gastrin can be detected through various methods like gastrin stain and antibodies such as anti-gastrin are often used for this purpose.
Biological function summary

Gastrin impacts digestion and is an important promoter of gastric acid secretion and mucosal growth. Gastrin does not assemble into larger protein complexes functioning instead as an independent peptide hormone. It exerts its effects by binding to the cholecystokinin B receptor (CCK-B) on target cells leading to increased intracellular calcium levels and activation of protein kinases. The interaction initiates a cascade of events facilitating digestion by optimizing the stomach's environment.

Pathways

Gastrin has a significant role in the gastrointestinal (GI) pathway where it activates the secretion of hydrochloric acid. It also influences the phosphoinositide pathway through its relationship with G-proteins. The hormonal action of gastrin involves several other proteins like somatostatin which serves as an inhibitory counterpart and histamine that works synergistically by promoting further acid secretion through different receptors.

Physicians often study gastrin in relation to conditions like Zollinger-Ellison syndrome and peptic ulcers. Zollinger-Ellison syndrome leads to excessive production of gastrin causing severe ulcerations and gastric acid secretions. Concurrently elevated gastrin levels sometimes associate with tumors like gastrinomas. The interplay between gastrin and H. pylori infection is pivotal for peptic ulcer development where bacteria-induced inflammation could alter gastrin's normal regulatory functions of acid secretion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
See full target information GAST
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Fundamental & clinical pharmacology 39:e13048 PubMed39777430

2025

Fluoxetine-induced downregulation of circMap2k1 signaling cascade to improve neurological function after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Langtao He,Hui Zhang,Jian Deng,Yibo He,Zhili Cai,Yitao He

The Journal of cell biology 223: PubMed38958606

2024

Calorie restriction activates a gastric Notch-FOXO1 pathway to expand ghrelin cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wendy M McKimpson,Sophia Spiegel,Maria Mukhanova,Michael Kraakman,Wen Du,Takumi Kitamoto,Junjie Yu,Zhaobin Deng,Utpal Pajvani,Domenico Accili

Nature communications 14:7978 PubMed38042929

2023

Role of PDGFRA cells and a CD55 PDGFRA fraction in the gastric mesenchymal niche.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Manieri,Guodong Tie,Ermanno Malagola,Davide Seruggia,Shariq Madha,Adrianna Maglieri,Kun Huang,Yuko Fujiwara,Kevin Zhang,Stuart H Orkin,Timothy C Wang,Ruiyang He,Neil McCarthy,Ramesh A Shivdasani

American journal of translational research 13:12929-12938 PubMed34956508

2021

Efficacy of Jianpiyangxue granule on gastrointestinal autonomic nerve dysfunction and its impact on adverse reactions.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqing Zhou,Shenggang Wang
View all publications
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