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AB223501

Anti-Gastrin antibody [SP318]

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(1 Publication)

Rabbit Recombinant Monoclonal Gastrin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GAS, GAST, Gastrin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody [SP318] (AB223501)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody [SP318] (AB223501)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling Gastrin with ab223501 at 1/100 dilution (1.08 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadic positive staining on the human stomach, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab223501 for 10 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody [SP318] (AB223501)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrin antibody [SP318] (AB223501)

Paraffin-embedded human stomach tissue stained for Gastrin using ab223501 at 1/100 dilution in immunohistochemical analysis.

  • Carrier free

    Anti-Gastrin antibody [SP318] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP318

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.6 Preservative: 0.099% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gastrin sometimes referred to as gastrin peptide is a hormone consisting of 34 amino acids with a molecular mass of approximately 3.8 kDa. This protein is mainly expressed in the G cells of the stomach lining specifically the antrum but also found in the duodenum and pancreas. Gastrin operates by stimulating the secretion of gastric acid from parietal cells in the stomach playing an important role in the digestive process. The presence of gastrin can be detected through various methods like gastrin stain and antibodies such as anti-gastrin are often used for this purpose.
Biological function summary

Gastrin impacts digestion and is an important promoter of gastric acid secretion and mucosal growth. Gastrin does not assemble into larger protein complexes functioning instead as an independent peptide hormone. It exerts its effects by binding to the cholecystokinin B receptor (CCK-B) on target cells leading to increased intracellular calcium levels and activation of protein kinases. The interaction initiates a cascade of events facilitating digestion by optimizing the stomach's environment.

Pathways

Gastrin has a significant role in the gastrointestinal (GI) pathway where it activates the secretion of hydrochloric acid. It also influences the phosphoinositide pathway through its relationship with G-proteins. The hormonal action of gastrin involves several other proteins like somatostatin which serves as an inhibitory counterpart and histamine that works synergistically by promoting further acid secretion through different receptors.

Physicians often study gastrin in relation to conditions like Zollinger-Ellison syndrome and peptic ulcers. Zollinger-Ellison syndrome leads to excessive production of gastrin causing severe ulcerations and gastric acid secretions. Concurrently elevated gastrin levels sometimes associate with tumors like gastrinomas. The interplay between gastrin and H. pylori infection is pivotal for peptic ulcer development where bacteria-induced inflammation could alter gastrin's normal regulatory functions of acid secretion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
See full target information GAST
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 24:424 PubMed39709453

2024

Epidermal growth factor receptor ligands enriched in follicular fluid exosomes promote oncogenesis of fallopian tube epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aye Aye Khine,Pao-Chu Chen,Ying-Hsi Chen,Sung-Chao Chu,Hsuan-Shun Huang,Tang-Yuan Chu
View all publications
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