AB231952

Anti-Gastrokine 1 antibody

Name: Anti-Gastrokine 1 antibody (ab231952) | Abcam
Brand: Abcam Limited
SKU: AB231952
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Gastrokine 1 antibody. Suitable for WB, IHC-P and reacts with Pig, Human, Recombinant full length protein - Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GKN1 aa 1 to C-terminus.

View Alternative Names

AMP18, CA11, UNQ489/PRO1005, GKN1, Gastrokine-1, 18 kDa antrum mucosa protein, Protein CA11, AMP-18

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

Formalin-fixed, paraffin-embedded human liver tissue stained for Gastrokine 1 using ab231952 at 20 μg/mL in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Gastrokine 1 using ab231952 at 20 μg/mL in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for Gastrokine 1 using ab231952 at 20 μg/mL in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Gastrokine 1 using ab231952 at 20 μg/mL in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Gastrokine 1 using ab231952 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Gastrokine 1 antibody (AB231952)

All lanes:

Western blot - Anti-Gastrokine 1 antibody (ab231952) at 1 µg/mL

All lanes:

Recombinant human Gastrokine 1 protein.

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 22 kDa

true

Supplier Data

Western blot - Anti-Gastrokine 1 antibody (AB231952)

All lanes:

Western blot - Anti-Gastrokine 1 antibody (ab231952) at 1 µg/mL

Lane 1:

Pig stomach tissue lysate.

Lane 2:

Rat stomach tissue lysate.

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 22 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GKN1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NS71

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gastrokine 1 also known as GKN1 is a protein with a molecular weight of approximately 18 kDa. It is expressed mainly in the gastric epithelium. This protein plays a mechanical role in maintaining gastric mucosal integrity. It has been associated with epithelial cell differentiation and proliferation a process important for stomach lining health. Gastrokine 1 interacts with other cellular components to stabilize and repair the gastric mucosa following injury or stress.

Biological function summary

This protein supports gastric mucosal defense mechanisms. It participates in maintaining the epithelial barrier against harmful digestive enzymes and pathogens. Gastrokine 1 is not part of a larger protein complex but operates independently to influence mucosal protection. Its ability to modulate cell turnover and repair processes highlights its importance in sustaining gastric homeostasis.

Pathways

Gastrokine 1 functions within the gastric mucosal protective pathway. It often relates to the signaling pathways involving epidermal growth factor receptor (EGFR) and inflammation mediation. The absence or reduction of GKN1 can disrupt these pathways impacting other proteins such as MUC5AC which also contribute to the protective mucosal barrier. The interaction between these proteins ensures a coordinated response in maintaining the stomach lining integrity.

Alterations in Gastrokine 1 expression have associations with gastric cancer and gastritis. Reduced levels of this protein often correspond with increased gastric epithelial damage and carcinogenesis risks. GKN1's association with diseases like gastric cancer makes it a potential biomarker. Additionally its interaction with Helicobacter pylori a bacterium linked to chronic gastritis and cancer demonstrates the protein's critical role in disease prevention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium.

See full target information GKN1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 11:1199 PubMed33441708

2021

High expression of hypoxia inducible factor 1α related with acquired resistant to EGFR tyrosine kinase inhibitors in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Jin,Feihua Huang,Xianrong Xu,Haidong He,Yingqing Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Gastrokine 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastrokine 1 antibody (AB231952)

Western blot - Anti-Gastrokine 1 antibody (AB231952)

Western blot - Anti-Gastrokine 1 antibody (AB231952)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise