AB11852

Anti-GATA1 antibody

Name: Anti-GATA1 antibody (ab11852) | Abcam
Brand: Abcam Limited
SKU: AB11852
Price: 450 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(27 Publications)

Anti-GATA1 antibody (ab11852) is a rabbit polyclonal antibody detecting GATA1 in Western Blot. Suitable for Human.

- Over 20 publications
- Trusted since 2004

View Alternative Names

ERYF1, GF1, GATA1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein, GATA-1, GF-1

1 Images

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Western blot - Anti-GATA1 antibody (AB11852)

Lane 1:

Western blot - Anti-GATA1 antibody (ab11852) at 1/400 dilution

Lane 2:

Western blot - Anti-GATA1 antibody (ab11852) at 1/800 dilution

Lane 3:

Negative control

All lanes:

K562 Human erythroleukamia cells

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human GATA1 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P15976

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Reactivity data

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Product details

What is this antibody validated in?
Anti-GATA1 antibody (ab11852) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of GATA1?
Anti-GATA1 (ab11852) specifically detects a band for GATA1 (UniProt: P15976) at a molecular weight of 43kDa.

Trusted by the scientific community
Anti-GATA1 (ab11852) was first used in a scientific publication in 2004 and has been cited over 20 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 1% BSA, 0.0268% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATA1 also known as GATA-binding factor 1 is a transcription factor weighing approximately 42 kDa. This protein binds to the DNA sequence "GATA" within the regulatory regions of target genes to control their expression. GATA1 is mostly expressed in hematopoietic cells especially erythroid cells megakaryocytes eosinophils and mast cells. Its specific expression pattern allows it to regulate genes essential for the development and function of these cell types.

Biological function summary

GATA1 regulates gene expression and is essential for erythroid and megakaryocyte differentiation. GATA1 belongs to a family of GATA transcription factors and can form regulatory complexes with other proteins such as FOG1 (Friend of GATA1) and TAL1. These complexes facilitate the binding of GATA1 to DNA enhancing its transcriptional activity on target gene sets necessary for blood cell maturation and function.

Pathways

GATA1 participates in the erythropoiesis and megakaryopoiesis pathways. It directly regulates several genes and coordinates signals with factors like GATA2 and RUNX1 which are other key proteins involved in these pathways. Through these interactions GATA1 ensures the correct development of red blood cells and platelets maintaining hematopoietic homeostasis in the organism.

Abnormal GATA1 function is linked to blood-related disorders. Mutations or dysregulations of GATA1 can lead to diseases such as X-linked thrombocytopenia with dyserythropoietic anemia which involves low platelet count and ineffective red blood cell production. Moreover GATA1 mutations are associated with certain leukemias particularly acute megakaryoblastic leukemia. GATA1's dysfunction in these diseases often occurs in conjunction with abnormalities in related proteins like GATA2 influencing disease progression and phenotype.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator or repressor which serves as a general switch factor for erythroid development (PubMed : 35030251). It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed : 24245781).

See full target information GATA1

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:20587 PubMed36447001

2022

Mechanism of KIT gene regulation by GATA1 lacking the N-terminal domain in Down syndrome-related myeloid disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Rika Kanezaki,Tsutomu Toki,Kiminori Terui,Tomohiko Sato,Akie Kobayashi,Ko Kudo,Takuya Kamio,Shinya Sasaki,Koji Kawaguchi,Kenichiro Watanabe,Etsuro Ito

Nature genetics 54:1417-1426 PubMed35941187

2022

HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Huang,Scott A Peslak,Ren Ren,Eugene Khandros,Kunhua Qin,Cheryl A Keller,Belinda Giardine,Henry W Bell,Xianjiang Lan,Malini Sharma,John R Horton,Osheiza Abdulmalik,Stella T Chou,Junwei Shi,Merlin Crossley,Ross C Hardison,Xiaodong Cheng,Gerd A Blobel

Nature genetics 53:1177-1186 PubMed34341563

2021

Activation of γ-globin gene expression by GATA1 and NF-Y in hereditary persistence of fetal hemoglobin.

Applications

Unspecified application

Species

Unspecified reactive species

Phillip A Doerfler,Ruopeng Feng,Yichao Li,Lance E Palmer,Shaina N Porter,Henry W Bell,Merlin Crossley,Shondra M Pruett-Miller,Yong Cheng,Mitchell J Weiss

Nature communications 12:4439 PubMed34290235

2021

Reactivation of a developmentally silenced embryonic globin gene.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew J King,Duantida Songdej,Damien J Downes,Robert A Beagrie,Siyu Liu,Megan Buckley,Peng Hua,Maria C Suciu,A Marieke Oudelaar,Lars L P Hanssen,Danuta Jeziorska,Nigel Roberts,Stephanie J Carpenter,Helena Francis,Jelena Telenius,Aude-Anais Olijnik,Jacqueline A Sharpe,Jacqueline Sloane-Stanley,Jennifer Eglinton,Mira T Kassouf,Stuart H Orkin,Len A Pennacchio,James O J Davies,Jim R Hughes,Douglas R Higgs,Christian Babbs

Nature communications 12:3806 PubMed34155213

2021

A gain-of-function single nucleotide variant creates a new promoter which acts as an orientation-dependent enhancer-blocker.

Applications

Unspecified application

Species

Unspecified reactive species

Yavor K Bozhilov,Damien J Downes,Jelena Telenius,A Marieke Oudelaar,Emmanuel N Olivier,Joanne C Mountford,Jim R Hughes,Richard J Gibbons,Douglas R Higgs

Cell death & disease 12:223 PubMed33637692

2021

Regulation of MYB by distal enhancer elements in human myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjia Li,Penglei Jiang,Kai Cheng,Zehui Zhang,Shuyu Lan,Xiaoxia Li,Lirong Zhao,Yucheng Wang,Xiang Wang,Jing Chen,Tao Ji,Bingshe Han,Junfang Zhang

Blood 137:1327-1339 PubMed33512425

2021

Dynamic CTCF binding directly mediates interactions among cis-regulatory elements essential for hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Qi,Li Cheng,Xing Tang,Yanghua He,Yichao Li,Tiffany Yee,Dewan Shrestha,Ruopeng Feng,Peng Xu,Xin Zhou,Shondra Pruett-Miller,Ross C Hardison,Mitchell J Weiss,Yong Cheng

Life science alliance 4: PubMed33397648

2021

Different mutant RUNX1 oncoproteins program alternate haematopoietic differentiation trajectories.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie G Kellaway,Peter Keane,Benjamin Edginton-White,Kakkad Regha,Ella Kennett,Constanze Bonifer

Scientific reports 10:19055 PubMed33149232

2020

Inhibition of red blood cell development by arsenic-induced disruption of GATA-1.

Applications

Unspecified application

Species

Unspecified reactive species

Xixi Zhou,Sebastian Medina,Alicia M Bolt,Haikun Zhang,Guanghua Wan,Huan Xu,Fredine T Lauer,Shu Chun Wang,Scott W Burchiel,Ke Jian Liu

The EMBO journal 39:e104749 PubMed32525588

2020

CCR5 deficiency impairs CD4 T-cell memory responses and antigenic sensitivity through increased ceramide synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Martín-Leal,Raquel Blanco,Josefina Casas,María E Sáez,Elena Rodríguez-Bovolenta,Itziar de Rojas,Carina Drechsler,Luis Miguel Real,Gemma Fabrias,Agustín Ruíz,Mario Castro,Wolfgang Wa Schamel,Balbino Alarcón,Hisse M van Santen,Santos Mañes

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