Anti-GATA1 antibody [EP2819Y]

• WB

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Western blot - Anti-GATA1 antibody [EP2819Y] (AB76121)

False colour image of Western blot : Anti-GATA1 antibody [EP2819Y] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab76121 was shown to bind specifically to GATA1. A band was observed at 47 kDa in wild-type K562 cell lysates with no signal observed at this size in GATA1 knockout cell line ab285360 (knockout cell lysate ab289686). To generate this image, wild-type and GATA1 knockout K562 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-GATA1 antibody [EP2819Y] (ab76121) at 1/1000 dilution

Lane 1:

Wild-type K562 cell lysate at 20 µg

Lane 2:

GATA1 knockout K562 cell lysate at 20 µg

Lane 3:

HL-60 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

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