Rabbit Polyclonal GATA1 antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GATA1.
Images
Publications
Filter by
- Haematologica :2019XPO1 regulates erythroid differentiation and is a new target for the treatment of β-thalassemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlavia Guillem,Michaël Dussiot,Elia Colin,Thunwarat Suriyun,Jean Benoit Arlet,Nicolas Goudin,Guillaume Marcion,Renaud Seigneuric,Sebastien Causse,Patrick Gonin,Marc Gastou,Marc Deloger,Julien Rossignol,Mathilde Lamarque,Zakia Bellaid Choucair,Emilie Fleur Gautier,Sarah Ducamp,Julie Vandekerckhove,Ivan C Moura,Thiago T Maciel,Carmen Garrido,Xiuli An,Patrick Mayeux,Narla Mohandas,Geneviève Courtois,Olivier HerminePubMed 31753924
- Blood advances 1:1959-19762017The severe phenotype of Diamond-Blackfan anemia is modulated by heat shock protein 70.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarc Gastou,Sarah Rio,Michaël Dussiot,Narjesse Karboul,Hélène Moniz,Thierry Leblanc,Margaux Sevin,Patrick Gonin,Jérome Larghéro,Carmen Garrido,Anupama Narla,Narla Mohandas,William Vainchenker,Olivier Hermine,Eric Solary,Lydie Da CostaPubMed 29296843
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GATA1. The exact immunogen used to generate this antibody is proprietary information. Database link P15976
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Transcriptional activator or repressor which serves as a general switch factor for erythroid development (PubMed:35030251). It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).
Alternative names
ERYF1, GF1, GATA1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein, GATA-1, GF-1
Recommended products
Rabbit Polyclonal GATA1 antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GATA1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GATA1. The exact immunogen used to generate this antibody is proprietary information. Database link P15976
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GATA1 also known as GATA-binding factor 1 is a transcription factor weighing approximately 42 kDa. This protein binds to the DNA sequence "GATA" within the regulatory regions of target genes to control their expression. GATA1 is mostly expressed in hematopoietic cells especially erythroid cells megakaryocytes eosinophils and mast cells. Its specific expression pattern allows it to regulate genes essential for the development and function of these cell types.
- Biological function summary
GATA1 regulates gene expression and is essential for erythroid and megakaryocyte differentiation. GATA1 belongs to a family of GATA transcription factors and can form regulatory complexes with other proteins such as FOG1 (Friend of GATA1) and TAL1. These complexes facilitate the binding of GATA1 to DNA enhancing its transcriptional activity on target gene sets necessary for blood cell maturation and function.
- Pathways
GATA1 participates in the erythropoiesis and megakaryopoiesis pathways. It directly regulates several genes and coordinates signals with factors like GATA2 and RUNX1 which are other key proteins involved in these pathways. Through these interactions GATA1 ensures the correct development of red blood cells and platelets maintaining hematopoietic homeostasis in the organism.
- Associated diseases and disorders
Abnormal GATA1 function is linked to blood-related disorders. Mutations or dysregulations of GATA1 can lead to diseases such as X-linked thrombocytopenia with dyserythropoietic anemia which involves low platelet count and ineffective red blood cell production. Moreover GATA1 mutations are associated with certain leukemias particularly acute megakaryoblastic leukemia. GATA1's dysfunction in these diseases often occurs in conjunction with abnormalities in related proteins like GATA2 influencing disease progression and phenotype.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody - N-terminal (ab173816)
Immunofluorescent analysis of formalin-fixed, permeabilized U2OS cells, labeling GATA1 with ab173816 at 1/200 dilution (green, middle panel) . Cells were washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Nuclei (blue, left panel) were stained with Hoechst 33342 dye.
Western blot - Anti-GATA1 antibody - N-terminal (ab173816)
4-20% Tris-HCl polyacrylamide gel.
All lanes: Western blot - Anti-GATA1 antibody - N-terminal (ab173816) at 1/500 dilution
Lane 1: NCCIT whole cell lysate at 25 µg
Lane 2: NTERRA whole cell lysate at 25 µg
Lane 3: K562 whole cell lysate at 25 µg
Lane 4: HeLa whole cell lysate at 25 µg
SecondaryAll lanes: goat anti-rabbit-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody - N-terminal (ab173816)
Paraffin embedded human tonsil tissue stained for GATA1 using ab173816 at 1/100 dilution in immunohistochemical analysis. Negative control without primary antibody is seen on the left.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com