AB28816

Anti-GATA1 (phospho S142) antibody

Name: Anti-GATA1 (phospho S142) antibody (ab28816) | Abcam
Brand: Abcam Limited
SKU: AB28816
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal GATA1 phospho S142 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GATA1 phospho S142.

View Alternative Names

ERYF1, GF1, GATA1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein, GATA-1, GF-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 (phospho S142) antibody (AB28816)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 (phospho S142) antibody (AB28816)

ab28816 at (1 : 50-1 : 100), staining paraffin embedded human breast carcinoma. Left : Using GATA1 antibody (ab28816); Right : antibody preincubated with synthesized phosphopeptide.

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Western blot - Anti-GATA1 (phospho S142) antibody (AB28816)

All lanes:

Western blot - Anti-GATA1 (phospho S142) antibody (ab28816) at 1/500 dilution

All lanes:

K562 cells

Predicted band size: 43 kDa

Observed band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GATA1 phospho S142. The exact immunogen used to generate this antibody is proprietary information.

P15976

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

After immunogen affinity purification the antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATA1 also known as GATA-binding factor 1 is a transcription factor weighing approximately 42 kDa. This protein binds to the DNA sequence "GATA" within the regulatory regions of target genes to control their expression. GATA1 is mostly expressed in hematopoietic cells especially erythroid cells megakaryocytes eosinophils and mast cells. Its specific expression pattern allows it to regulate genes essential for the development and function of these cell types.

Biological function summary

GATA1 regulates gene expression and is essential for erythroid and megakaryocyte differentiation. GATA1 belongs to a family of GATA transcription factors and can form regulatory complexes with other proteins such as FOG1 (Friend of GATA1) and TAL1. These complexes facilitate the binding of GATA1 to DNA enhancing its transcriptional activity on target gene sets necessary for blood cell maturation and function.

Pathways

GATA1 participates in the erythropoiesis and megakaryopoiesis pathways. It directly regulates several genes and coordinates signals with factors like GATA2 and RUNX1 which are other key proteins involved in these pathways. Through these interactions GATA1 ensures the correct development of red blood cells and platelets maintaining hematopoietic homeostasis in the organism.

Abnormal GATA1 function is linked to blood-related disorders. Mutations or dysregulations of GATA1 can lead to diseases such as X-linked thrombocytopenia with dyserythropoietic anemia which involves low platelet count and ineffective red blood cell production. Moreover GATA1 mutations are associated with certain leukemias particularly acute megakaryoblastic leukemia. GATA1's dysfunction in these diseases often occurs in conjunction with abnormalities in related proteins like GATA2 influencing disease progression and phenotype.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Transcriptional activator or repressor which serves as a general switch factor for erythroid development (PubMed : 35030251). It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed : 24245781).

See full target information GATA1 phospho S142

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:81 PubMed38360723

2024

CD24 induced cellular quiescence-like state and chemoresistance in ovarian cancer cells via miR-130a/301a-dependent CDK19 downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yeonsue Jang,Suki Kang,Hyun Ho Han,Baek Gil Kim,Nam Hoon Cho

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