AB194912

Anti-GATA1 (phospho S310) antibody

Name: Anti-GATA1 (phospho S310) antibody (ab194912) | Abcam
Brand: Abcam Limited
SKU: AB194912
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal GATA1 phospho S310 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GATA1 phospho S310.

View Alternative Names

ERYF1, GF1, GATA1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein, GATA-1, GF-1

2 Images

Supplier Data

Western blot - Anti-GATA1 (phospho S310) antibody (AB194912)

All lanes:

Western blot - Anti-GATA1 (phospho S310) antibody (ab194912) at 1/500 dilution

Lane 1:

HT29 cell extract, untreated

Lane 2:

HT29 cell extract, treated with Anisomycin

Predicted band size: 43 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-GATA1 (phospho S310) antibody (AB194912)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GATA1 (phospho S310) antibody (AB194912)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling GATA1 (phospho S310) with ab194912 at 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human GATA1 phospho S310. The exact immunogen used to generate this antibody is proprietary information.

P15976

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATA1 also known as GATA-binding factor 1 is a transcription factor weighing approximately 42 kDa. This protein binds to the DNA sequence "GATA" within the regulatory regions of target genes to control their expression. GATA1 is mostly expressed in hematopoietic cells especially erythroid cells megakaryocytes eosinophils and mast cells. Its specific expression pattern allows it to regulate genes essential for the development and function of these cell types.

Biological function summary

GATA1 regulates gene expression and is essential for erythroid and megakaryocyte differentiation. GATA1 belongs to a family of GATA transcription factors and can form regulatory complexes with other proteins such as FOG1 (Friend of GATA1) and TAL1. These complexes facilitate the binding of GATA1 to DNA enhancing its transcriptional activity on target gene sets necessary for blood cell maturation and function.

Pathways

GATA1 participates in the erythropoiesis and megakaryopoiesis pathways. It directly regulates several genes and coordinates signals with factors like GATA2 and RUNX1 which are other key proteins involved in these pathways. Through these interactions GATA1 ensures the correct development of red blood cells and platelets maintaining hematopoietic homeostasis in the organism.

Abnormal GATA1 function is linked to blood-related disorders. Mutations or dysregulations of GATA1 can lead to diseases such as X-linked thrombocytopenia with dyserythropoietic anemia which involves low platelet count and ineffective red blood cell production. Moreover GATA1 mutations are associated with certain leukemias particularly acute megakaryoblastic leukemia. GATA1's dysfunction in these diseases often occurs in conjunction with abnormalities in related proteins like GATA2 influencing disease progression and phenotype.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator or repressor which serves as a general switch factor for erythroid development (PubMed : 35030251). It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed : 24245781).

See full target information GATA1 phospho S310

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 9:182 PubMed34772466

2021

Tyrosine 136 phosphorylation of α-synuclein aggregates in the Lewy body dementia brain: involvement of serine 129 phosphorylation by casein kinase 2.

Applications

Unspecified application

Species

Unspecified reactive species

Kazunori Sano,Yasushi Iwasaki,Yuta Yamashita,Keiichi Irie,Masato Hosokawa,Katsuya Satoh,Kenichi Mishima

Frontiers in pharmacology 12:710722 PubMed34603025

2021

Intervention of Gastrodin in Type 2 Diabetes Mellitus and Its Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Bai,Ke Mo,Guirong Wang,Wanling Chen,Wei Zhang,Yibo Guo,Zhirong Sun

View all publications

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