Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free

• IP

Unknown

Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (AB239959)

GATA2 was immunoprecipitated from 0.35 mg K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate 10 μg with ab109241 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab109241 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate 10 μg

Lane 2 : ab109241 IP in K-562 cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab109241 in K562 cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109241).

All lanes:

Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (<a href='/en-us/products/primary-antibodies/gata2-antibody-epr28222-ab109241'>ab109241</a>)

Predicted band size: 50 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (AB239959)

Purified ab109241 at 1/30 dilution (2 μg) immunoprecipitating GATA2 in K-562 whole cell lysate.
Lane 1 (input) : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg
Lane 2 (+) : ab109241 + K-562 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109241 in K-562 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 51 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109241).

All lanes:

Immunoprecipitation - Anti-GATA2 antibody [EPR2822(2)] (<a href='/en-us/products/primary-antibodies/gata2-antibody-epr28222-ab109241'>ab109241</a>)

Predicted band size: 33 kDa,50 kDa

Observed band size: 50 kDa

false

• ChIP

Lab

ChIP - Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (AB239959)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109241).

Chromatin was prepared from K-562 (Human chronic myelogenous leukemia lymphoblast) cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then 1% formaldehyde for 10min.

The ChIP was performed with 25 µg of chromatin, 5 µg of ab109241 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR ( Sybr green approach).

Primers and probes are from paper PMID : 22036566.

• WB

Unknown

Western blot - Anti-GATA2 antibody [EPR2822(2)] - BSA and Azide free (AB239959)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109241).

All lanes:

Western blot - Anti-GATA2 antibody [EPR2822(2)] (<a href='/en-us/products/primary-antibodies/gata2-antibody-epr28222-ab109241'>ab109241</a>) at 1/1000 dilution

Lane 1:

bEnd.3 (Mouse brain endothelioma) whole cell lysate at 15 µg

Lane 2:

Mouse placenta lysate at 15 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 51 kDa

false