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AB138451

Anti-GATA2 (phospho S401) antibody

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(1 Publication)

Rabbit Polyclonal GATA2 phospho S401 antibody. Suitable for WB and reacts with African green monkey samples. Cited in 1 publication.

View Alternative Names

Endothelial transcription factor GATA-2, GATA-binding protein 2, GATA2

1 Images
Western blot - Anti-GATA2 (phospho S401) antibody (AB138451)
  • WB

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Western blot - Anti-GATA2 (phospho S401) antibody (AB138451)

All lanes:

Western blot - Anti-GATA2 (phospho S401) antibody (ab138451) at 1/500 dilution

All lanes:

Extracts from COS7 cells treated with 20 ng/ml of TNF for 5 minutes.

Predicted band size: 50 kDa,51 kDa,54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

African green monkey

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab138451 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATA2 also known as GATA binding protein 2 is a transcription factor with a molecular mass of approximately 50 kDa. It plays an important mechanical role in regulating gene expression by binding to the DNA sequence "GATA". GATA2 is expressed in various tissues including hematopoietic cells the nervous system and the endothelium. It influences cell fate decisions impacting the development and function of multiple tissues and cell types.
Biological function summary

GATA2 governs hematopoiesis and vascular development. It operates within multiprotein complexes to control the transcription of genes necessary for the development and differentiation of hematopoietic stem cells. GATA2's presence aids in maintaining normal functions of these cells ensuring they proliferate and differentiate correctly. Its regulatory role extends to the vascular system where GATA2 controls endothelial cell function and blood vessel formation.

Pathways

Hematopoiesis and vascular development significantly involve GATA2. It participates in the Notch signaling pathway influencing cell differentiation and proliferation along with other transcription factors like GATA1 and FOG1. The pathway interplay facilitates normal blood cell production and proper vascularization emphasizing its important role in cellular development processes.

Defective GATA2 function links to immunodeficiency syndromes and acute myeloid leukemia (AML). Mutations in the GATA2 gene disrupt normal blood cell development often resulting in increased susceptibility to infections and hematologic malignancies. It interacts with proteins such as GATA1 in these pathological states highlighting its significance in both immune function and malignant transformation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
See full target information GATA2 phospho S401
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 290:10764-74 PubMed25784556

2015

Carrageenan Inhibits Insulin Signaling through GRB10-mediated Decrease in Tyr(P)-IRS1 and through Inflammation-induced Increase in Ser(P)307-IRS1.

Applications

WB

Species

Unspecified reactive species

Sumit Bhattacharyya,Leo Feferman,Joanne K Tobacman
View all publications
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Product promise

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