AB268065

Anti-GATA3 antibody [GATA3/2444]

Name: Anti-GATA3 antibody [GATA3/2444] (ab268065) | Abcam
Brand: Abcam Limited
SKU: AB268065
Price: 530 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal GATA3 antibody. Suitable for Protein Array, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GATA3 aa 350 to C-terminus.

View Alternative Names

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 antibody [GATA3/2444] (AB268065)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 antibody [GATA3/2444] (AB268065)

Formalin-fixed, paraffin-embedded human Breast Carcinoma tissue stained for GATA-3 using ab268065 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-GATA3 antibody [GATA3/2444] (AB268065)

Protein Array

Unknown

Protein Array - Anti-GATA3 antibody [GATA3/2444] (AB268065)

Protein Array containing more than 19,000 full-length human proteins using ab268065. Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GATA3/2444

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, Protein Array

applications

Immunogen

Recombinant Fragment Protein within Human GATA3 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P23771

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from Bioreactor concentrate

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATA3 also known as GATA-binding factor 3 is a transcription factor with a molecular mass of about 50 kDa. It belongs to the GATA family of zinc finger transcription factors. GATA3 is mainly expressed in the immune system especially in T-cells as well as in the nervous system and mammary glands. It plays a role in the regulation of critical developmental processes and cell lineage commitment through its binding to the 'GATA' motif in the promoter regions of target genes.

Biological function summary

GATA3 functions as a regulator of gene expression involved in various differentiation processes. It is not a solitary molecule; it interacts with other proteins to form transcriptional complexes. In T-helper type 2 (Th2) cells GATA3 is necessary for Th2 cell differentiation and cytokine production. Its expression is vital for the development of the nervous system and mammary gland morphogenesis highlighting its role in diverse biological contexts.

Pathways

GATA3 plays a significant role in the Th2 differentiation pathway and is linked to the immune response. It acts in association with other transcription factors like STAT6 and T-bet. GATA3 promotes the transcription of Th2 cytokines such as IL-4 IL-5 and IL-13 while suppressing Th1 responses. This balance orchestrated by GATA3 is important for immune homeostasis and the body's ability to respond to infections and allergens.

The dysregulation of GATA3 is associated with atopic diseases and breast cancer. Aberrant expression of GATA3 is often observed in allergic conditions due to its role in Th2 cell differentiation and cytokine expression. In the context of breast cancer GATA3 levels can impact tumor progression and are correlated with estrogen receptor status. Additionally its relation with other breast cancer-related proteins like L50 underlines its importance in cancer biology and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity). Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.

See full target information GATA3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 28:530 PubMed39290955

2024

Male breast cancer metastasising to the liver: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Mingming Jiang,Linyun Li,Hongjie Liu,Hua Xie

Free neuropathology 2: PubMed37284625

2021

Friedreich cardiomyopathy is a desminopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Arnulf H Koeppen,Rahman F Rafique,Joseph E Mazurkiewicz,Steven Pelech,Catherine Sutter,Qishan Lin,Jiang Qian

View all publications

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