AB240298

Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal GATA3 phospho S308 antibody. Carrier free. Suitable for IP, Dot, WB, IHC-P and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples. Cited in 1 publication.

View Alternative Names

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on trophoblast cells of Human placenta is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of Human transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371). Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling GATA3 (phospho S308) with ab186371 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear staining on rat stomach without Lambda Protein Phosphatase treatment (image A). No signal was detected when tissues were treated with Lambda Protein Phosphatase treatment (image B). The section was incubated with ab186371 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371). Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling GATA3 (phospho S308) with ab186371 at 1/50 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear staining on mouse stomach without Lambda Protein Phosphatase treatment (image A). No signal was detected when tissues were treated with Lambda Protein Phosphatase treatment (image B). The section was incubated with ab186371 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Lab

Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371). Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] (<a href='/en-us/products/primary-antibodies/gata3-phospho-s308-antibody-epr18118-ab186371'>ab186371</a>) at 1/1000 dilution

Lane 1:

Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Lane 2:

EL4 treated with 0.5 mM 8-Bromo-cAMP for 6 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 48 kDa

false

Exposure time: 70s

Supplier Data

Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

GATA3 (phospho S308) was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with 1mM 8-Bromo-cAMP for 6 hours with ab186371 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab186371 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

Lane 1 : Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours, 10 μg (Input).

Lane 2 : ab186371 IP in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab186371 in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371).

All lanes:

Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] (<a href='/en-us/products/primary-antibodies/gata3-phospho-s308-antibody-epr18118-ab186371'>ab186371</a>)

Predicted band size: 47 kDa

Observed band size: 48 kDa

false

Supplier Data

Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Dot blot analysis of GATA3 (phospho S308) peptide (Lane 1) and non-phospho peptide (Lane 2) labeled using ab186371 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST..

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371).

Lab

Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371). Dot blot analysis of GATA3 (phospho S308) using ab186371 at 1 : 1000 (1.121 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody (ab97051) at 1 : 100000 dilution. Lane 1 : Human GATA3 (phospho Ser316) peptide Lane 2 : Human GATA3 (phospho Thr315) peptide Lane 3 : Human GATA3 (phospho Ser308) peptide Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

Unconjugated

Anti-GATA3 (phospho S308) antibody [EPR18118]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18118

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IP, IHC-P, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen sequence for this antibody is identical to the sequence around S340 of GATA2 (UniProt P23769); however the phosphorylation of GATA2 on S340 has not been reported.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab240298 is the carrier-free version of ab186371.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATA3 also known as GATA-binding factor 3 is a transcription factor with a molecular mass of about 50 kDa. It belongs to the GATA family of zinc finger transcription factors. GATA3 is mainly expressed in the immune system especially in T-cells as well as in the nervous system and mammary glands. It plays a role in the regulation of critical developmental processes and cell lineage commitment through its binding to the 'GATA' motif in the promoter regions of target genes.

Biological function summary

GATA3 functions as a regulator of gene expression involved in various differentiation processes. It is not a solitary molecule; it interacts with other proteins to form transcriptional complexes. In T-helper type 2 (Th2) cells GATA3 is necessary for Th2 cell differentiation and cytokine production. Its expression is vital for the development of the nervous system and mammary gland morphogenesis highlighting its role in diverse biological contexts.

Pathways

GATA3 plays a significant role in the Th2 differentiation pathway and is linked to the immune response. It acts in association with other transcription factors like STAT6 and T-bet. GATA3 promotes the transcription of Th2 cytokines such as IL-4 IL-5 and IL-13 while suppressing Th1 responses. This balance orchestrated by GATA3 is important for immune homeostasis and the body's ability to respond to infections and allergens.

The dysregulation of GATA3 is associated with atopic diseases and breast cancer. Aberrant expression of GATA3 is often observed in allergic conditions due to its role in Th2 cell differentiation and cytokine expression. In the context of breast cancer GATA3 levels can impact tumor progression and are correlated with estrogen receptor status. Additionally its relation with other breast cancer-related proteins like L50 underlines its importance in cancer biology and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity). Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.

See full target information GATA3 phospho S308

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of histochemistry : EJH 65: PubMed34587716

2021

Long noncoding RNA Meg3 mediates ferroptosis induced by oxygen and glucose deprivation combined with hyperglycemia in rat brain microvascular endothelial cells, through modulating the p53/GPX4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chen,Yan Huang,Pingping Xia,Fan Zhang,Longyan Li,E Wang,Qulian Guo,Zhi Ye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Western blot - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (AB240298)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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