Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free

• IP

Unknown

Immunoprecipitation - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (AB275254)

This data was developed using ab256782, the same antibody clone in a different buffer formulation.

GATA4 was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 μg with ab256782 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256782 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Huh7 whole cell lysate 10 μg.

Lane 2 : ab256782 IP in Huh7 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab256782 in Huh7 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 49 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 31903133). 20 kDa band in Huh7 whole cell lysate is consistent with what has been described in the literature (PMID : 25501827).

All lanes:

Immunoprecipitation - Anti-GATA4 antibody [EPR23691-12] (<a href='/en-us/products/primary-antibodies/gata4-antibody-epr23691-12-ab256782'>ab256782</a>)

Predicted band size: 44 kDa

false

• WB

Lab

Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (AB275254)

This data was developed using ab256782, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-GATA4 antibody [EPR23691-12] (<a href='/en-us/products/primary-antibodies/gata4-antibody-epr23691-12-ab256782'>ab256782</a>) at 1/1000 dilution

All lanes:

Human liver cancer tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 44 kDa

false

• WB

Lab

Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (AB275254)

This data was developed using ab256782, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The lane 1 of blot was developed using a higher sensitivity ECL substrate.

The molecular weight observed is consistent with what has been described in the literature (PMID : 28758902, 16940177, 18426912, 31903133).

20 kDa band in HepG2 whole cell lysate is consistent with what has been described in the literature (PMID : 25501827).

Negative control : HeLa (PMID : 24696446, 24352556, 31322241)

Exposure time : Lane 1 : 101 seconds; Lane 2-4 : 3 minutes.

All lanes:

Western blot - Anti-GATA4 antibody [EPR23691-12] (<a href='/en-us/products/primary-antibodies/gata4-antibody-epr23691-12-ab256782'>ab256782</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

Huh7 (human hepatocellar carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 44 kDa

false