Skip to main content

Rabbit Recombinant Monoclonal GATA4 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (AB275254), expandable thumbnail
  • Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (AB275254), expandable thumbnail
  • Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (AB275254), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Tested
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed:24000169, PubMed:27984724, PubMed:35182466). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression (PubMed:27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase (PubMed:15734735).

Alternative names

Transcription factor GATA-4, GATA-binding factor 4, GATA4

Recommended products

Rabbit Recombinant Monoclonal GATA4 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR23691-12
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab275254 is the carrier-free version of Anti-GATA4 antibody [EPR23691-12] ab256782.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GATA4 also known as GATA-binding protein 4 is a transcription factor that plays a significant role in gene expression regulation. It weighs approximately 44 kDa. This protein expresses mainly in cells of the heart gonads and liver. GATA4 functions through binding to the GATA sequence on DNA regulating various genes. The protein is well known for its involvement in cardiac development and function.

Biological function summary

GATA4 interacts with other transcription factors and co-factors to regulate developmental processes. It often forms complexes with proteins such as NKX2-5 and TBX5 influencing the expression of cardiac-specific genes. These interactions lead to tissue-specific gene expression control important in the differentiation and functioning of heart cells. GATA4 is also involved in the regulation of genes responsible for cell growth and apoptosis contributing to tissue homeostasis.

Pathways

GATA4 is a notable component of cardiac development and cellular differentiation pathways. It plays an integral role in the Wnt signaling pathway influencing heart development through gene transcription modification. GATA4 also collaborates with proteins such as MEF2 and BMP2 coordinating cardiac muscle differentiation. These pathways highlight its pivotal role in proper cardiovascular formation and function.

Associated diseases and disorders

GATA4 mutations and dysregulation have links to congenital heart defects such as atrial septal defect (ASD). This condition associates with altered gene expression during heart development leading to structural abnormalities. GATA4 also relates to conditions like hypertrophic cardiomyopathy where its interaction with proteins like MYH7 suggests a complex network influencing cardiac hypertrophy. Understanding these connections is essential for developing targeted therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254), expandable thumbnail

    Immunoprecipitation - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)

    This data was developed using Anti-GATA4 antibody [EPR23691-12] ab256782, the same antibody clone in a different buffer formulation.

    GATA4 was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 μg with Anti-GATA4 antibody [EPR23691-12] ab256782 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-GATA4 antibody [EPR23691-12] ab256782 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Huh7 whole cell lysate 10 μg.

    Lane 2: Anti-GATA4 antibody [EPR23691-12] ab256782 IP in Huh7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GATA4 antibody [EPR23691-12] ab256782 in Huh7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 49 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 31903133). 20 kDa band in Huh7 whole cell lysate is consistent with what has been described in the literature (PMID: 25501827).

    All lanes: Immunoprecipitation - Anti-GATA4 antibody [EPR23691-12] (Anti-GATA4 antibody [EPR23691-12] ab256782)

    Predicted band size: 44 kDa

  • Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254), expandable thumbnail

    Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)

    This data was developed using Anti-GATA4 antibody [EPR23691-12] ab256782, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lane 1 of blot was developed using a higher sensitivity ECL substrate.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28758902, 16940177, 18426912, 31903133).

    20 kDa band in HepG2 whole cell lysate is consistent with what has been described in the literature (PMID: 25501827).

    Negative control: HeLa (PMID: 24696446, 24352556, 31322241)

    Exposure time: Lane 1: 101 seconds; Lane 2-4: 3 minutes.

    All lanes: Western blot - Anti-GATA4 antibody [EPR23691-12] (Anti-GATA4 antibody [EPR23691-12] ab256782) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 2: Huh7 (human hepatocellar carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate at 20 µg

    Lane 4: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa

  • Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254), expandable thumbnail

    Western blot - Anti-GATA4 antibody [EPR23691-12] - BSA and Azide free (ab275254)

    This data was developed using Anti-GATA4 antibody [EPR23691-12] ab256782, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-GATA4 antibody [EPR23691-12] (Anti-GATA4 antibody [EPR23691-12] ab256782) at 1/1000 dilution

    All lanes: Human liver cancer tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 44 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com