Anti-GATA4 antibody [EPR26718-103]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GATA4 antibody [EPR26718-103] (AB307823)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labelling GATA4 with ab307823 at 1/50 (9.82 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in Huh7 cell line.Negative control : HeLa (PMID : 24696446).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (AB307823)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling GATA4 with ab307823 at 1/500 (0.982 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human testis. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-GATA4 antibody [EPR26718-103] (AB307823)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / Huh7(human hepatocellular carcinoma epithelial cell, Right) cells labelling GATA4 with ab307823 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (AB307823)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GATA4 with ab307823 at 1/500 (0.982 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control : No staining on human skeletal muscle. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (AB307823)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling GATA4 with ab307823 at 1/4000 (0.123 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control : No staining on rat skeletal muscle. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (AB307823)

Immunohistochemical analysis of paraffin-embedded (A) Mouse stomach (tissue labeling GATA4 with ab307823 at 1/4000 (0.123 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on (A) mouse stomach (PMID : 34111600). No staining on (B) mouse colon (PMID : 18405344). The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (AB307823)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling GATA4 with ab307823 at 1/4000 (0.123 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on rat testis. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (AB307823)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling GATA4 with ab307823 at 1/4000 (0.123 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on mouse testis (PMID : 30793514). The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (AB307823)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling GATA4 with ab307823 at 1/4000 (0.123 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control : No staining on mouse skeletal muscle. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

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Immunoprecipitation - Anti-GATA4 antibody [EPR26718-103] (AB307823)

GATA4 was immunoprecipitated from 0.35 mg Mouse P7 testis tissue lysate 10 ug with ab307823 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307823 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse P7 testis tissue lysate 10 ug
Lane 2 : ab307823 IP in Mouse P7 testis tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307823 in mouse P7 testis tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 81 seconds

All lanes:

Immunoprecipitation - Anti-GATA4 antibody [EPR26718-103] (ab307823) at 1/1000 dilution

All lanes:

Mouse P7 testis cell

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 56 kDa

false

Exposure time: 81s

• WB

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Western blot - Anti-GATA4 antibody [EPR26718-103] (AB307823)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure : Lanes 1-3 : 180 seconds; Lane 4 : 10 seconds; Lanes 5-6 : 180 seconds.

All lanes:

Western blot - Anti-GATA4 antibody [EPR26718-103] (ab307823) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse P7 ovary tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Rat P7 testis tissue lysate at 20 µg

Lane 5:

Rat P7 ovary tissue lysate at 20 µg

Lane 6:

Rat skeletal muscle tissue lysate at 20 µg

Observed band size: 56 kDa

false