Anti-GATA4 antibody [EPR26718-103] 20 ul size (ab307823)

Rabbit Recombinant Monoclonal GATA4 antibody. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), WB, IP and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-GATA4 antibody [EPR26718-103] (AB307823), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	Flow Cyt (Intra)	WB	IP	IHC-Fr
Human	Tested	Tested	Tested	Not recommended	Expected	Not recommended
Mouse	Tested	Expected	Expected	Tested	Tested	Not recommended
Rat	Tested	Expected	Expected	Tested	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Target data

See full target information Gata4

Function

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:35182466). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression (By similarity). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (PubMed:9584153). Promotes cardiac myocyte enlargement (By similarity). Required during testicular development (By similarity). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase (By similarity).

Alternative names

Gata-4, Gata4, Transcription factor GATA-4, GATA-binding factor 4

Recommended products

Rabbit Recombinant Monoclonal GATA4 antibody. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26718-103
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GATA4 also known as GATA-binding protein 4 is a transcription factor that plays a significant role in gene expression regulation. It weighs approximately 44 kDa. This protein expresses mainly in cells of the heart gonads and liver. GATA4 functions through binding to the GATA sequence on DNA regulating various genes. The protein is well known for its involvement in cardiac development and function.

Biological function summary

GATA4 interacts with other transcription factors and co-factors to regulate developmental processes. It often forms complexes with proteins such as NKX2-5 and TBX5 influencing the expression of cardiac-specific genes. These interactions lead to tissue-specific gene expression control important in the differentiation and functioning of heart cells. GATA4 is also involved in the regulation of genes responsible for cell growth and apoptosis contributing to tissue homeostasis.

Pathways

GATA4 is a notable component of cardiac development and cellular differentiation pathways. It plays an integral role in the Wnt signaling pathway influencing heart development through gene transcription modification. GATA4 also collaborates with proteins such as MEF2 and BMP2 coordinating cardiac muscle differentiation. These pathways highlight its pivotal role in proper cardiovascular formation and function.

Associated diseases and disorders

GATA4 mutations and dysregulation have links to congenital heart defects such as atrial septal defect (ASD). This condition associates with altered gene expression during heart development leading to structural abnormalities. GATA4 also relates to conditions like hypertrophic cardiomyopathy where its interaction with proteins like MYH7 suggests a complex network influencing cardiac hypertrophy. Understanding these connections is essential for developing targeted therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Flow Cytometry (Intracellular) - Anti-GATA4 antibody [EPR26718-103] (ab307823)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / Huh7(human hepatocellular carcinoma epithelial cell, Right) cells labelling GATA4 with ab307823 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-GATA4 antibody [EPR26718-103] (ab307823)
GATA4 was immunoprecipitated from 0.35 mg Mouse P7 testis tissue lysate 10 ug with ab307823 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307823 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

Lane 1: Mouse P7 testis tissue lysate 10 ug

Lane 2: abab307823 IP in Mouse P7 testis tissue lysate

Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307823 in mouse P7 testis tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 81 seconds
All lanes: Immunoprecipitation - Anti-GATA4 antibody [EPR26718-103] (ab307823) at 1/1000 dilution
All lanes: Mouse P7 testis cell
Secondary
All lanes: Immunoprecipitation at 1/5000 dilution
Observed band size: 56 kDa
Exposure time: 81s
Immunocytochemistry/ Immunofluorescence - Anti-GATA4 antibody [EPR26718-103] (ab307823)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labelling GATA4 with ab307823 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in Huh7 cell line.Negative control: HeLa (PMID: 24696446).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling GATA4 with ab307823 at 1/4000 (0.123 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on rat skeletal muscle. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling GATA4 with ab307823 at 1/4000 (0.123 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on mouse skeletal muscle. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GATA4 with ab307823 at 1/500 (0.982 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on human skeletal muscle. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling GATA4 with ab307823 at 1/4000 (0.123 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on rat testis. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)
Immunohistochemical analysis of paraffin-embedded (A) Mouse stomach (tissue labeling GATA4 with ab307823 at 1/4000 (0.123 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on (A) mouse stomach (PMID: 34111600). No staining on (B) mouse colon (PMID: 18405344). The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling GATA4 with ab307823 at 1/4000 (0.123 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on mouse testis (PMID: 30793514). The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody [EPR26718-103] (ab307823)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling GATA4 with ab307823 at 1/500 (0.982 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human testis. The section was incubated with ab307823 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-GATA4 antibody [EPR26718-103] (ab307823)
Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure: Lanes 1-3: 180 seconds; Lane 4: 10 seconds; Lanes 5-6:180 seconds.
All lanes: Western blot - Anti-GATA4 antibody [EPR26718-103] (ab307823) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg
Lane 2: Mouse P7 ovary tissue lysate at 20 µg
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg
Lane 4: Rat P7 testis tissue lysate at 20 µg
Lane 5: Rat P7 ovary tissue lysate at 20 µg
Lane 6: Rat skeletal muscle tissue lysate at 20 µg
Observed band size: 56 kDa