Anti-Gata6 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gata6 antibody (AB175349)

Immunohistochemical analysis of Human stomach tissue labeling Gata6 with ab175349 at 1/100 dilution. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Following labeling, tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Gata6 antibody (AB175349)

Immunofluorescent analysis of HeLa cells labeling Gata6 with ab175349 at 1/200 dilution (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Following detection with the primary antibody, the cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1/200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.

• WB

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Western blot - Anti-Gata6 antibody (AB175349)

All lanes:

Western blot - Anti-Gata6 antibody (ab175349) at 1/1000 dilution

Lane 1:

293T cell lysate at 25 µg

Lane 2:

MCF7 cell lysate at 25 µg

Lane 3:

K562 cell lysate at 25 µg

Lane 4:

3T3L1 cell lysate at 25 µg

Secondary

All lanes:

Goat anti-rabbit-HRP at 1/15000 dilution

Predicted band size: 60 kDa

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