AB22600

Anti-Gata6 antibody

Name: Anti-Gata6 antibody (ab22600) | Abcam
Brand: Abcam Limited
SKU: AB22600
Price: 495 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(33 Publications)

Rabbit Polyclonal GATA6 antibody. Suitable for WB and reacts with Mouse samples. Cited in 33 publications.

View Alternative Names

Transcription factor GATA-6, GATA-binding factor 6, GATA6

1 Images

Project7407****

Western blot - Anti-Gata6 antibody (AB22600)

All lanes:

Western blot - Anti-Gata6 antibody (ab22600) at 1 µg/mL

All lanes:

D3 ES Cells Day 15 EB (Gata6 +ve) at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution

Predicted band size: 60 kDa

Observed band size: 45 kDa,73 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Abcam recommends using milk as the blocking agent.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATA6 also known as GATA-binding protein 6 functions mechanically as a transcription factor in the GATA family. It has a molecular mass of approximately 60 kDa. GATA6 binds to the DNA sequence "GATA" present in the promoters of target genes regulating gene expression. This protein is expressed widely but is particularly noted in endodermal-derived tissues and plays a significant role in cellular differentiation and organogenesis during development.

Biological function summary

GATA6 plays an important role in endodermal lineage specification and development. It participates in complex networks regulating the differentiation and function of various organs such as the lungs heart and gut. This transcription factor interacts with other proteins in intricate transcriptional complexes to modulate the expression of genes involved in cell growth and development. GATA6 aids tissue-specific gene regulation facilitating organ development and function.

Pathways

GATA6 is integral to at least the TGF-beta signaling pathway and the Wnt signaling pathway. In these pathways GATA6 regulates the expression of genes that are essential for cell proliferation differentiation and apoptosis. It works closely with other key proteins like SMAD and β-catenin to drive downstream genetic programs that underpin cellular development and tissue homeostasis.

GATA6 mutations have been associated with congenital heart defects and pancreatic agenesis. Alterations in GATA6 function can disrupt its interaction with proteins such as NKX2-5 leading to developmental anomalies in the cardiovascular system. Similarly its dysregulation links to improper pancreatic development contributing to diabetes-related conditions. GATA6's dysfunction in these areas highlights its significance in developmental biology and disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator (PubMed : 19666519, PubMed : 22750565, PubMed : 22824924, PubMed : 27756709). Regulates SEMA3C and PLXNA2 (PubMed : 19666519). Involved in gene regulation specifically in the gastric epithelium (PubMed : 9315713). May regulate genes that protect epithelial cells from bacterial infection (PubMed : 16968778). Involved in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). In human skin, controls several physiological processes contributing to homeostasis of the upper pilosebaceous unit. Triggers ductal and sebaceous differentiation as well as limits cell proliferation and lipid production to prevent hyperseborrhoea. Mediates the effects of retinoic acid on sebocyte proliferation, differentiation and lipid production. Also contributes to immune regulation of sebocytes and antimicrobial responses by modulating the expression of anti-inflammatory genes such as IL10 and pro-inflammatory genes such as IL6, TLR2, TLR4, and IFNG. Activates TGFB1 signaling which controls the interfollicular epidermis fate (PubMed : 33082341).

See full target information GATA6

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:11544 PubMed40185811

2025

Topological data analysis of pattern formation of human induced pluripotent stem cell colonies.

Applications

Unspecified application

Species

Unspecified reactive species

Iryna Hartsock,Eunbi Park,Jack Toppen,Peter Bubenik,Elena S Dimitrova,Melissa L Kemp,Daniel A Cruz

Development, growth & differentiation 67:23-32 PubMed39723530

2024

The polycomb protein complex interacts with GATA-6/PPARα to inhibit α-MHC expression.

Applications

Unspecified application

Species

Unspecified reactive species

Fei-Fei Dai,Jing Chen,Zhen Ma,Ming-Hui Yang,Tong Sun,Juan Ma,Meng-Jiao Zhou,Zhi-Ru Wei,Yunzeng Zou,Shoutao Zhang,Ming-Xi Zang

Bioactive materials 45:181-200 PubMed39651397

2024

Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Tang,Fuxiang Wei,Weihua Qiao,Xing Chen,Chenyang Ji,Wanzhi Yang,Xinyu Zhang,Sihan Chen,Yanyan Wu,Mingxing Jiang,Chenyu Ma,Weiqiang Shen,Qi Dong,Hong Cao,Minghui Xie,Ziwen Cai,Li Xu,Jiawei Shi,Nianguo Dong,Junwei Chen,Ning Wang

Nature communications 13:2237 PubMed35469023

2022

Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2.

Applications

Unspecified application

Species

Unspecified reactive species

Ma'ayan Israeli,Yaara Finkel,Yfat Yahalom-Ronen,Nir Paran,Theodor Chitlaru,Ofir Israeli,Inbar Cohen-Gihon,Moshe Aftalion,Reut Falach,Shahar Rotem,Uri Elia,Ital Nemet,Limor Kliker,Michal Mandelboim,Adi Beth-Din,Tomer Israely,Ofer Cohen,Noam Stern-Ginossar,Adi Bercovich-Kinori

Cell death discovery 7:220 PubMed34446696

2021

Long noncoding RNA LINC00261 upregulates ITIH5 to impair tumorigenic ability of pancreatic cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Zou,Hengpeng He,Zhiguo Li,Ou Chen,Xiukun Jia,Hao Zhang

Frontiers in veterinary science 8:680539 PubMed34212020

2021

Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Pérez-Gómez,Leopoldo González-Brusi,Pablo Bermejo-Álvarez,Priscila Ramos-Ibeas

Mechanisms of development 162:103611 PubMed32376282

2020

HYDIN loss-of-function inhibits GATA4 expression and enhances atrial septal defect risk.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Cao,Junying Guo,Jinping Zhang,Li Li,Hui Wang,Wenjun Ren,Lihong Jiang

Cell proliferation 53:e12782 PubMed32196806

2020

Derivation of porcine extraembryonic endoderm-like cells from blastocysts.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Shuang Wu,Yang Yu,Heng Zhang,Renyue Wei,Jiawei Lv,Mingming Cai,Xu Yang,Yu Zhang,Zhonghua Liu

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:5754-5766 PubMed32103545

2020

Loss of GATA6 expression promotes lymphatic metastasis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chanjuan Wang,Qinghua Liu,Ming Huang,Qianghua Zhou,Xiaoyu Zhang,Jingtong Zhang,Ruihui Xie,Yanqi Yu,Shang Chen,Jianbing Fan,Xu Chen

Pathology, research and practice 215:152593 PubMed31471104

2019

Inhibition of PRRX2 suppressed colon cancer liver metastasis via inactivation of Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Xiao Chai,Li-Guo Sun,Fu-Hong Dai,Hong-Sheng Shao,Ning-Gang Zheng,Hong-Yi Cai

View all publications

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Product promise

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Anti-Gata6 antibody

Western blot - Anti-Gata6 antibody (AB22600)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (33)

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