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AB224391

Anti-GATAD2B antibody

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(3 Publications)

Rabbit Polyclonal GATAD2B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GATAD2B aa 50-200.

View Alternative Names

KIAA1150, GATAD2B, Transcriptional repressor p66-beta, GATA zinc finger domain-containing protein 2B, p66/p68

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2B antibody (AB224391)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2B antibody (AB224391)

Paraffin-embedded human lymph node tissue stained for GATAD2B using ab224391 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GATAD2B antibody (AB224391)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GATAD2B antibody (AB224391)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for GATAD2B (green) using ab224391 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2B antibody (AB224391)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2B antibody (AB224391)

Immunohistochemical analysis of human cerebellum labeling GATAD2B with ab224391 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2B antibody (AB224391)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2B antibody (AB224391)

Immunohistochemical analysis of human cerebral cortex labeling GATAD2B with ab224391 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-GATAD2B antibody (AB224391)
  • WB

Supplier Data

Western blot - Anti-GATAD2B antibody (AB224391)

All lanes:

Western blot - Anti-GATAD2B antibody (ab224391) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 65 kDa

false

Western blot - Anti-GATAD2B antibody (AB224391)
  • WB

Supplier Data

Western blot - Anti-GATAD2B antibody (AB224391)

All lanes:

Western blot - Anti-GATAD2B antibody (ab224391) at 0.4 µg/mL

Lane 1:

SK-BR-3 transfected with control siRNA cell lysate

Lanes 2 - 3:

SK-BR-3 transfected with target specific siRNA probe cell lysate

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GATAD2B aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q8WXI9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATAD2B also known as p66β is a protein which forms part of the nucleosome remodeling and deacetylase (NuRD) complex. The protein has a mass of approximately 66 kDa. It is expressed in various human tissues including brain and skeletal muscle. GATAD2B plays a vital role in transcriptional repression by recruiting chromatin remodeling factors which influence gene expression regulation.
Biological function summary

GATAD2B influences chromatin structure and gene expression. It functions as an integral component of the NuRD complex which combines ATP-dependent chromatin remodeling with histone deacetylase activity. The interaction of GATAD2B with other NuRD components such as MBD2 and CHD3 demonstrates its role in modifying the accessibility of DNA to transcription factors impacting cellular activities like differentiation.

Pathways

Other components of the NuRD complex work together with GATAD2B in the regulation of the Wnt signaling pathway and the Notch signaling pathway. These pathways are important for cellular proliferation and differentiation. GATAD2B has functional connections with other proteins including REST and HDAC1 and these connections indicate its involvement in these pathways that shape developmental processes and cellular responses.

GATAD2B is significant in connection with neurodevelopmental disorders. Pathogenic mutations in the GATAD2B gene have been linked to GATAD2B-associated neurodevelopmental disorder. It is also associated with intellectual disability syndromes. GATAD2B shows interactions with proteins such as TCF4 and SMARCA2 which are also linked to similar neurodevelopmental conditions illustrating its impact on brain function and development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor (PubMed : 12183469, PubMed : 16415179). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). Enhances MBD2-mediated repression (PubMed : 12183469, PubMed : 16415179). Efficient repression requires the presence of GATAD2A (PubMed : 16415179). Targets MBD3 to discrete loci in the nucleus (PubMed : 11756549). May play a role in synapse development (PubMed : 23644463).
See full target information GATAD2B
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:6365 PubMed39075094

2024

Cell fate decision by a morphogen-transcription factor-chromatin modifier axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Ming,Lihui Lin,Jiajun Li,Linlin Wu,Shicai Fang,Tao Huang,Yu Fu,Dong Liu,Wenhui Zhang,Chen Li,Yongzheng Yang,Yi Huang,Yue Qin,Junqi Kuang,Xingnan Huang,Liman Guo,Xiaofei Zhang,Jing Liu,Jiekai Chen,Chengchen Zhao,Bo Wang,Duanqing Pei

Nature communications 14:2846 PubMed37208322

2023

The NuRD complex cooperates with SALL4 to orchestrate reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wang,Chen Li,Jin Ming,Linlin Wu,Shicai Fang,Yi Huang,Lihui Lin,He Liu,Junqi Kuang,Chengchen Zhao,Xingnan Huang,Huijian Feng,Jing Guo,Xuejie Yang,Liman Guo,Xiaofei Zhang,Jiekai Chen,Jing Liu,Ping Zhu,Duanqing Pei

Cancer management and research 12:8833-8845 PubMed33061579

2020

Long Non-Coding RNA GAS5 Suppresses Tumor Progression and Enhances the Radiosensitivity of Prostate Cancer Through the miR-320a/RAB21 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiulong Ma,Zhongwei Wang,Hongtao Ren,Xing Bao,Yang Zhang,Baofeng Wang,Dongli Ruan
View all publications
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Product promise

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