AB76925

Anti-GATAD2B antibody

Name: Anti-GATAD2B antibody (ab76925) | Abcam
Brand: Abcam Limited
SKU: AB76925
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal GATAD2B antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GATAD2B aa 50-100.

View Alternative Names

KIAA1150, GATAD2B, Transcriptional repressor p66-beta, GATA zinc finger domain-containing protein 2B, p66/p68

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2B antibody (AB76925)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2B antibody (AB76925)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling p66 beta with ab76925 at 1/200 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-GATAD2B antibody (AB76925)

ab76925, at 1μg/ml, staining GATAD2B in HeLa whole cell lysate immunoprecipitated using ab76925 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

All lanes:

Immunoprecipitation - Anti-GATAD2B antibody (ab76925)

Predicted band size: 65 kDa

false

Unknown

Western blot - Anti-GATAD2B antibody (AB76925)

All lanes:

Western blot - Anti-GATAD2B antibody (ab76925) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 65 kDa

Observed band size: 130 kDa,72 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human GATAD2B aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q8WXI9

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab76925 was affinity purified using an epitope specific to GATAD2B immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATAD2B also known as p66β is a protein which forms part of the nucleosome remodeling and deacetylase (NuRD) complex. The protein has a mass of approximately 66 kDa. It is expressed in various human tissues including brain and skeletal muscle. GATAD2B plays a vital role in transcriptional repression by recruiting chromatin remodeling factors which influence gene expression regulation.

Biological function summary

GATAD2B influences chromatin structure and gene expression. It functions as an integral component of the NuRD complex which combines ATP-dependent chromatin remodeling with histone deacetylase activity. The interaction of GATAD2B with other NuRD components such as MBD2 and CHD3 demonstrates its role in modifying the accessibility of DNA to transcription factors impacting cellular activities like differentiation.

Pathways

Other components of the NuRD complex work together with GATAD2B in the regulation of the Wnt signaling pathway and the Notch signaling pathway. These pathways are important for cellular proliferation and differentiation. GATAD2B has functional connections with other proteins including REST and HDAC1 and these connections indicate its involvement in these pathways that shape developmental processes and cellular responses.

GATAD2B is significant in connection with neurodevelopmental disorders. Pathogenic mutations in the GATAD2B gene have been linked to GATAD2B-associated neurodevelopmental disorder. It is also associated with intellectual disability syndromes. GATAD2B shows interactions with proteins such as TCF4 and SMARCA2 which are also linked to similar neurodevelopmental conditions illustrating its impact on brain function and development.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional repressor (PubMed : 12183469, PubMed : 16415179). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). Enhances MBD2-mediated repression (PubMed : 12183469, PubMed : 16415179). Efficient repression requires the presence of GATAD2A (PubMed : 16415179). Targets MBD3 to discrete loci in the nucleus (PubMed : 11756549). May play a role in synapse development (PubMed : 23644463).

See full target information GATAD2B

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:2453-2485 PubMed38719994

2024

The GATAD2B-NuRD complex drives DNA:RNA hybrid-dependent chromatin boundary formation upon DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Zhichao Liu,Kamal Ajit,Yupei Wu,Wei-Guo Zhu,Monika Gullerova

Cell death & disease 14:382 PubMed37380643

2023

GATA zinc finger protein p66β promotes breast cancer cell migration by acting as a co-activator of Snail.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuqun Zou,Li Ma,Yihong Zhang,Qun Zhang,Chu Xu,Dan Zhang,Yimin Chu,Jie Zhang,Mengying Li,Hui Zhang,Jiamin Wang,Chicheng Peng,Gang Wei,Yingjie Wu,Zhaoyuan Hou,Hao Jia

Cell death and differentiation 29:1042-1054 PubMed34815549

2021

MAP2K6 remodels chromatin and facilitates reprogramming by activating Gatad2b-phosphorylation dependent heterochromatin loosening.

Applications

Unspecified application

Species

Unspecified reactive species

Guangsuo Xing,Zichao Liu,Luyuan Huang,Danyun Zhao,Tao Wang,Hao Yuan,Yi Wu,Linpeng Li,Qi Long,Yanshuang Zhou,Zhihong Hao,Yang Liu,Jianghuan Lu,Shiting Li,Jieying Zhu,Bo Wang,Junwei Wang,Jing Liu,Jiekai Chen,Duanqing Pei,Xingguo Liu,Keshi Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GATAD2B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATAD2B antibody (AB76925)

Immunoprecipitation - Anti-GATAD2B antibody (AB76925)

Western blot - Anti-GATAD2B antibody (AB76925)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise