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AB228937

Anti-GATM antibody

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(2 Publications)

Rabbit Polyclonal GATM antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GATM.

View Alternative Names

AGAT, GATM, L-arginine:glycine amidinotransferase, Transamidinase

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human GATM. The exact immunogen used to generate this antibody is proprietary information.

P50440

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATM also known as glycine amidinotransferase or L-arginine:glycine amidinotransferase functions as an enzyme responsible for the catalysis of the first step in creatine biosynthesis. This enzyme has a molecular mass of approximately 49 kDa. GATM is mostly expressed in the kidney pancreas and liver. In these tissues it catalyzes the transfer of an amidino group from L-arginine to glycine forming guanidinoacetate the immediate precursor for creatine.
Biological function summary

Glycine amidinotransferase participates in important metabolic processes by forming guanidinoacetate. This compound collaborates with S-adenosylmethionine to eventually form creatine an important molecule important for storing and distributing energy in cells. GATM does not function as part of a larger complex; it works independently to perform its enzymatic reaction. Its biological activity directly impacts muscle function and also affects organ systems that depend on energy homeostasis.

Pathways

GATM plays a significant role in creatine synthesis connecting to the energy metabolism pathway. The creatine biosynthesis pathway begins with GATM's production of guanidinoacetate and concludes with the formation of creatine after a conversion facilitated by the enzyme GAMT (guanidinoacetate N-methyltransferase). GATM functions as an upstream regulator impacting ATP supply in tissues where high-energy demands occur like in muscle cells.

Researchers associate GATM with conditions related to energy metabolism deficiencies such as certain myopathies and guanidinoacetate methyltransferase deficiency. These conditions arise when alterations in creatine synthesis disrupt normal cellular energy dynamics. GATM mutations can affect overall energy availability leading to muscle and neurological symptoms. The enzyme GAMT interacts with GATM within these metabolic contexts illustrating a close connection in the pathology of related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transamidinase that catalyzes the transfer of the amidino group of L-arginine onto the amino moiety of acceptor metabolites such as glycine, beta-alanine, gamma-aminobutyric acid (GABA) and taurine yielding the corresponding guanidine derivatives (PubMed : 16820567, PubMed : 27233232, PubMed : 36543883, PubMed : 3800397). Catalyzes the rate-limiting step of creatine biosynthesis, namely the transfer of the amidino group from L-arginine to glycine to generate guanidinoacetate, which is then methylated by GAMT to form creatine. Provides creatine as a source for ATP generation in tissues with high energy demands, in particular skeletal muscle, heart and brain (Probable) (PubMed : 27233232, PubMed : 36543883, PubMed : 3800397, PubMed : 9266688).
See full target information GATM
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell chemical biology 29:1470-1481.e31 PubMed36070758

2022

Exploring the target scope of KEAP1 E3 ligase-based PROTACs.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyan Du,Jie Jiang,Nathaniel J Henning,Nozhat Safaee,Eriko Koide,Radosław P Nowak,Katherine A Donovan,Hojong Yoon,Inchul You,Hong Yue,Nicholas A Eleuteri,Zhixiang He,Zhengnian Li,Hubert T Huang,Jianwei Che,Behnam Nabet,Tinghu Zhang,Eric S Fischer,Nathanael S Gray

Biomolecules & therapeutics : PubMed33408288

2021

Suppression of by miR-132-3p Accelerates Cyst Formation by Up-Regulating ROS in Autosomal Dominant Polycystic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Seonju Choi,Do Yeon Kim,Yejin Ahn,Eun Ji Lee,Jong Hoon Park
View all publications
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Product promise

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