AB238605

Anti-GATM antibody

Name: Anti-GATM antibody (ab238605) | Abcam
Brand: Abcam Limited
SKU: AB238605

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(2 Publications)

Rabbit Polyclonal GATM antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GATM aa 100-450.

View Alternative Names

AGAT, GATM, L-arginine:glycine amidinotransferase, Transamidinase

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody (AB238605)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody (AB238605)

Paraffin-embedded human kidney tissue stained for GATM using ab238605 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody (AB238605)

Paraffin-embedded human liver tissue stained for GATM using ab238605 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GATM aa 100-450. The exact immunogen used to generate this antibody is proprietary information.

P50440

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATM also known as glycine amidinotransferase or L-arginine:glycine amidinotransferase functions as an enzyme responsible for the catalysis of the first step in creatine biosynthesis. This enzyme has a molecular mass of approximately 49 kDa. GATM is mostly expressed in the kidney pancreas and liver. In these tissues it catalyzes the transfer of an amidino group from L-arginine to glycine forming guanidinoacetate the immediate precursor for creatine.

Biological function summary

Glycine amidinotransferase participates in important metabolic processes by forming guanidinoacetate. This compound collaborates with S-adenosylmethionine to eventually form creatine an important molecule important for storing and distributing energy in cells. GATM does not function as part of a larger complex; it works independently to perform its enzymatic reaction. Its biological activity directly impacts muscle function and also affects organ systems that depend on energy homeostasis.

Pathways

GATM plays a significant role in creatine synthesis connecting to the energy metabolism pathway. The creatine biosynthesis pathway begins with GATM's production of guanidinoacetate and concludes with the formation of creatine after a conversion facilitated by the enzyme GAMT (guanidinoacetate N-methyltransferase). GATM functions as an upstream regulator impacting ATP supply in tissues where high-energy demands occur like in muscle cells.

Researchers associate GATM with conditions related to energy metabolism deficiencies such as certain myopathies and guanidinoacetate methyltransferase deficiency. These conditions arise when alterations in creatine synthesis disrupt normal cellular energy dynamics. GATM mutations can affect overall energy availability leading to muscle and neurological symptoms. The enzyme GAMT interacts with GATM within these metabolic contexts illustrating a close connection in the pathology of related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transamidinase that catalyzes the transfer of the amidino group of L-arginine onto the amino moiety of acceptor metabolites such as glycine, beta-alanine, gamma-aminobutyric acid (GABA) and taurine yielding the corresponding guanidine derivatives (PubMed : 16820567, PubMed : 27233232, PubMed : 36543883, PubMed : 3800397). Catalyzes the rate-limiting step of creatine biosynthesis, namely the transfer of the amidino group from L-arginine to glycine to generate guanidinoacetate, which is then methylated by GAMT to form creatine. Provides creatine as a source for ATP generation in tissues with high energy demands, in particular skeletal muscle, heart and brain (Probable) (PubMed : 27233232, PubMed : 36543883, PubMed : 3800397, PubMed : 9266688).

See full target information GATM

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2403517 PubMed39119937

2024

Creatine Promotes Endometriosis by Inducing Ferroptosis Resistance via Suppression of PrP.

Applications

Unspecified application

Species

Unspecified reactive species

Siman Chen,Xiaoqian Ma,Yukai Liu,Zhiqi Zhong,Chunyan Wei,Mingqing Li,Xiaoyong Zhu

Science advances 7:eabf6063 PubMed34705506

2021

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Molly M Wilson,Céline Callens,Matthieu Le Gallo,Svetlana Mironov,Qiong Ding,Amandine Salamagnon,Tony E Chavarria,Roselyne Viel,Abena D Peasah,Arjun Bhutkar,Sophie Martin,Florence Godey,Patrick Tas,Hong Soon Kang,Philippe P Juin,Anton M Jetten,Jane E Visvader,Robert A Weinberg,Massimo Attanasio,Claude Prigent,Jacqueline A Lees,Vincent J Guen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GATM antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody (AB238605)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody (AB238605)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise