AB119269

Anti-GATM antibody [OTI1C9]

Name: Anti-GATM antibody [OTI1C9] (ab119269) | Abcam
Brand: Abcam Limited
SKU: AB119269
Price: 520 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Mouse Monoclonal GATM antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human, Transfected cell line - Human, Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GATM.

View Alternative Names

AGAT, GATM, L-arginine:glycine amidinotransferase, Transamidinase

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody [OTI1C9] (AB119269)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody [OTI1C9] (AB119269)

Paraffin-embedded human liver tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody [OTI1C9] (AB119269)

Paraffin-embedded human pancreas tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-GATM antibody [OTI1C9] (AB119269)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GATM antibody [OTI1C9] (AB119269)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with GATM (Red), or an empty vector control plasmid (Blue), stained with ab119269 at 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody [OTI1C9] (AB119269)

Paraffin-embedded human liver carcinoma tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody [OTI1C9] (AB119269)

Paraffin-embedded human kidney tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GATM antibody [OTI1C9] (AB119269)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GATM antibody [OTI1C9] (AB119269)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY GATM, stained for GATM (Green) using ab119269 at 1/100 dilution in ICC/IF.

Unknown

Western blot - Anti-GATM antibody [OTI1C9] (AB119269)

All lanes:

Western blot - Anti-GATM antibody [OTI1C9] (ab119269) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs at 5 µg

Lane 2:

HEK-293T lysate transfected with pCMV6-ENTRY GATM cDNA for 48 hrs at 5 µg

Predicted band size: 48 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1C9

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GATM.

P50440

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATM also known as glycine amidinotransferase or L-arginine:glycine amidinotransferase functions as an enzyme responsible for the catalysis of the first step in creatine biosynthesis. This enzyme has a molecular mass of approximately 49 kDa. GATM is mostly expressed in the kidney pancreas and liver. In these tissues it catalyzes the transfer of an amidino group from L-arginine to glycine forming guanidinoacetate the immediate precursor for creatine.

Biological function summary

Glycine amidinotransferase participates in important metabolic processes by forming guanidinoacetate. This compound collaborates with S-adenosylmethionine to eventually form creatine an important molecule important for storing and distributing energy in cells. GATM does not function as part of a larger complex; it works independently to perform its enzymatic reaction. Its biological activity directly impacts muscle function and also affects organ systems that depend on energy homeostasis.

Pathways

GATM plays a significant role in creatine synthesis connecting to the energy metabolism pathway. The creatine biosynthesis pathway begins with GATM's production of guanidinoacetate and concludes with the formation of creatine after a conversion facilitated by the enzyme GAMT (guanidinoacetate N-methyltransferase). GATM functions as an upstream regulator impacting ATP supply in tissues where high-energy demands occur like in muscle cells.

Researchers associate GATM with conditions related to energy metabolism deficiencies such as certain myopathies and guanidinoacetate methyltransferase deficiency. These conditions arise when alterations in creatine synthesis disrupt normal cellular energy dynamics. GATM mutations can affect overall energy availability leading to muscle and neurological symptoms. The enzyme GAMT interacts with GATM within these metabolic contexts illustrating a close connection in the pathology of related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transamidinase that catalyzes the transfer of the amidino group of L-arginine onto the amino moiety of acceptor metabolites such as glycine, beta-alanine, gamma-aminobutyric acid (GABA) and taurine yielding the corresponding guanidine derivatives (PubMed : 16820567, PubMed : 27233232, PubMed : 36543883, PubMed : 3800397). Catalyzes the rate-limiting step of creatine biosynthesis, namely the transfer of the amidino group from L-arginine to glycine to generate guanidinoacetate, which is then methylated by GAMT to form creatine. Provides creatine as a source for ATP generation in tissues with high energy demands, in particular skeletal muscle, heart and brain (Probable) (PubMed : 27233232, PubMed : 36543883, PubMed : 3800397, PubMed : 9266688).

See full target information GATM

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 2: PubMed30979824

2019

Human CST suppresses origin licensing and promotes AND-1/Ctf4 chromatin association.

Applications

Unspecified application

Species

Unspecified reactive species

Yilin Wang,Kathryn S Brady,Benjamin P Caiello,Stephanie M Ackerson,Jason A Stewart

Journal of cellular biochemistry 116:2620-7 PubMed25926069

2015

Atorvastatin increases miR-124a expression: a mechanism of Gamt modulation in liver cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alisa Phulukdaree,Devapregasan Moodley,Sajidah Khan,Anil A Chuturgoon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GATM antibody [OTI1C9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody [OTI1C9] (AB119269)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody [OTI1C9] (AB119269)

Flow Cytometry (Intracellular) - Anti-GATM antibody [OTI1C9] (AB119269)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody [OTI1C9] (AB119269)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATM antibody [OTI1C9] (AB119269)

Immunocytochemistry/ Immunofluorescence - Anti-GATM antibody [OTI1C9] (AB119269)

Western blot - Anti-GATM antibody [OTI1C9] (AB119269)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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