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Rat Recombinant Monoclonal GBA2 antibody. Suitable for IHC-P and reacts with Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBA2 antibody [4A12] (AB252831), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBA2 antibody [4A12] (AB252831), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-FrIHC-P
Mouse
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
0.023 µg/mL
Notes

Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramides/GlcCers (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) to free glucose and ceramides (such as N-acylsphing-4-enine) (PubMed:17080196, PubMed:23250757). GlcCers are membrane glycosphingolipids that have a wide intracellular distribution (PubMed:23250757). They are the main precursors of more complex glycosphingolipids that play a role in cellular growth, differentiation, adhesion, signaling, cytoskeletal dynamics and membrane properties (PubMed:25803043). Also involved in the transglucosylation of cholesterol, transferring glucose from GlcCer, thereby modifying its water solubility and biological properties (PubMed:26724485, PubMed:32144204). Under specific conditions, may catalyze the reverse reaction, transferring glucose from cholesteryl-3-beta-D-glucoside to ceramide (such as N-acylsphing-4-enine) (PubMed:26724485, PubMed:32144204). May play a role in the metabolism of bile acids (PubMed:17080196). Able to hydrolyze bile acid 3-O-glucosides as well as to produce bile acid-glucose conjugates thanks to a bile acid glucosyl transferase activity (PubMed:17080196). Catalyzes the hydrolysis of galactosylceramides/GalCers (such as beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine), as well as galactosyl transfer between GalCers and cholesterol in vitro with lower activity compared with their activity against GlcCers (PubMed:32144204).

Alternative names

Kiaa1605, Gba2, Non-lysosomal glucosylceramidase, NLGase, Beta-glucocerebrosidase 2, Bile acid beta-glucosidase GBA2, Bile acid glucosyl transferase GBA2, Cholesterol glucosyltransferase GBA2, Cholesteryl-beta-glucosidase GBA2, Glucosylceramidase 2, Non-lysosomal cholesterol glycosyltransferase, Non-lysosomal galactosylceramidase, Non-lysosomal glycosylceramidase, Beta-glucosidase 2

Recommended products

Rat Recombinant Monoclonal GBA2 antibody. Suitable for IHC-P and reacts with Mouse samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
4A12
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GBA2 also known as non-lysosomal glucosylceramidase is an enzyme responsible for hydrolyzing glucosylceramide into glucose and ceramide. With a molecular mass approximately 118 kDa GBA2 localizes in the endoplasmic reticulum and the cytosol. It is widely expressed in various tissues including the liver brain and testis indicating its diverse physiological roles. Unlike GBA1 which functions in lysosomal degradation GBA2 operates outside lysosomes.

Biological function summary

GBA2 facilitates the metabolism of glycosphingolipids. It functions as part of lipid homeostasis ensuring the balance of ceramide levels. This enzyme plays a role in maintaining cellular functions that require precise lipid composition. Although not characterized as part of a larger complex GBA2 significantly impacts membrane dynamics and cellular signaling processes.

Pathways

GBA2 is integral to the glycosphingolipid metabolism pathway impacting cell signaling and membrane structure. Its activity influences sphingolipid metabolism connecting it with the sphingomyelinase pathway. In this context it relates to enzymes like ceramidases and sphingomyelinases which also contribute to sphingolipid breakdown and ceramide regulation.

Associated diseases and disorders

Research has associated GBA2 with conditions such as hereditary spastic paraplegia and Niemann-Pick disease type C. Mutations or dysregulation of GBA2 can disturb lipid metabolism leading to neurological impairments observed in these disorders. Connections between GBA2 and other proteins such as NPC1 which is involved in cholesterol transport highlight its relevance in complex metabolic disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBA2 antibody [4A12] (ab252831), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBA2 antibody [4A12] (ab252831)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling GBA2 with ab252831 at 0.023μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Membranous staining on mouse testis is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBA2 antibody [4A12] (ab252831), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBA2 antibody [4A12] (ab252831)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling GBA2 with ab252831 at 0.023μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on mouse cerebrum is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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