Rabbit Polyclonal GBA2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GBA2 aa 700 to C-terminus.
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Publications
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- The Journal of biological chemistry 295:5257-52772020Glucocerebrosidases catalyze a transgalactosylation reaction that yields a newly-identified brain sterol metabolite, galactosylated cholesterol.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHisako Akiyama,Mitsuko Ide,Yasuko Nagatsuka,Tomoko Sayano,Etsuro Nakanishi,Norihito Uemura,Kohei Yuyama,Yoshiki Yamaguchi,Hiroyuki Kamiguchi,Ryosuke Takahashi,Johannes M F G Aerts,Peter Greimel,Yoshio HirabayashiPubMed 32144204
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GBA2 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCG7
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramides/GlcCers (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) to free glucose and ceramides (such as N-acylsphing-4-enine) (PubMed:17105727, PubMed:30308956, PubMed:32144204). GlcCers are membrane glycosphingolipids that have a wide intracellular distribution (By similarity). They are the main precursors of more complex glycosphingolipids that play a role in cellular growth, differentiation, adhesion, signaling, cytoskeletal dynamics and membrane properties (By similarity). Involved in the transglucosylation of cholesterol, transfers glucose from GlcCer to cholesterol, thereby modifying its water solubility and biological properties (PubMed:32144204). Under specific conditions, may catalyze the reverse reaction, transferring glucose from cholesteryl-3-beta-D-glucoside to ceramide (such as N-acylsphing-4-enine) (Probable). May play a role in the metabolism of bile acids (PubMed:11489889, PubMed:17080196, PubMed:9111029). Able to hydrolyze bile acid 3-O-glucosides as well as to produce bile acid-glucose conjugates thanks to a bile acid glucosyl transferase activity (PubMed:11489889, PubMed:17080196, PubMed:9111029). Catalyzes the hydrolysis of galactosylceramides/GalCers (such as beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine), as well as the galactosyl transfer between GalCers and cholesterol in vitro with lower activity compared with their activity against GlcCers (PubMed:32144204).
Alternative names
KIAA1605, SPG46, AD035, GBA2, Non-lysosomal glucosylceramidase, NLGase, Beta-glucocerebrosidase 2, Bile acid beta-glucosidase GBA2, Bile acid glucosyl transferase GBA2, Cholesterol glucosyltransferase GBA2, Cholesteryl-beta-glucosidase GBA2, Glucosylceramidase 2, Non-lysosomal cholesterol glycosyltransferase, Non-lysosomal galactosylceramidase, Non-lysosomal glycosylceramidase, Beta-glucosidase 2
Recommended products
Rabbit Polyclonal GBA2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GBA2 aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GBA2 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCG7
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- reconstitute with water at 200µL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GBA2 also known as non-lysosomal glucosylceramidase is an enzyme responsible for hydrolyzing glucosylceramide into glucose and ceramide. With a molecular mass approximately 118 kDa GBA2 localizes in the endoplasmic reticulum and the cytosol. It is widely expressed in various tissues including the liver brain and testis indicating its diverse physiological roles. Unlike GBA1 which functions in lysosomal degradation GBA2 operates outside lysosomes.
- Biological function summary
GBA2 facilitates the metabolism of glycosphingolipids. It functions as part of lipid homeostasis ensuring the balance of ceramide levels. This enzyme plays a role in maintaining cellular functions that require precise lipid composition. Although not characterized as part of a larger complex GBA2 significantly impacts membrane dynamics and cellular signaling processes.
- Pathways
GBA2 is integral to the glycosphingolipid metabolism pathway impacting cell signaling and membrane structure. Its activity influences sphingolipid metabolism connecting it with the sphingomyelinase pathway. In this context it relates to enzymes like ceramidases and sphingomyelinases which also contribute to sphingolipid breakdown and ceramide regulation.
- Associated diseases and disorders
Research has associated GBA2 with conditions such as hereditary spastic paraplegia and Niemann-Pick disease type C. Mutations or dysregulation of GBA2 can disturb lipid metabolism leading to neurological impairments observed in these disorders. Connections between GBA2 and other proteins such as NPC1 which is involved in cholesterol transport highlight its relevance in complex metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-GBA2 antibody (ab205064)
All lanes: Western blot - Anti-GBA2 antibody (ab205064) at 1/500 dilution
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Predicted band size: 105 kDa
Observed band size: 98 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBA2 antibody (ab205064)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal colon tissue labeling GBA2 with ab205064 at 1/100 dilution.
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