AB276056

Anti-GBA3 antibody [008] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal GBA3 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human GBA3 aa 1 to C-terminus.

View Alternative Names

CBG, CBGL1, GBA3, Cytosolic beta-glucosidase, Cytosolic beta-glucosidase-like protein 1, Cytosolic galactosylceramidase, Cytosolic glucosylceramidase, Cytosolic glycosylceramidase, Glucosidase beta acid 3, Glucosylceramidase beta 3, Klotho-related protein, Cytosolic GCase, KLrP

2 Images

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Immunocytochemistry - Anti-GBA3 antibody [008] - BSA and Azide free (AB276056)

Immunofluorescent analysis of 4% PFA fixed, 0.1% Triton X-100 in PBS permeabilzed MCF7 (Human breast adenocarcinoma cell line) cells labeling GBA3 with ab276036 at 1/60 dilution, followed by Goat Anti-rabbit IgG Alexa Fluor 488-conjugated secondary antibody (green). Confocal image showing cytoplasm staining of MCF7 cells. Countstained with Alexa Fluor 546-conjugated phallotoxins (red) and DAPI (blue).

Flow Cytometry (Intracellular) - Anti-GBA3 antibody [008] - BSA and Azide free (AB276056)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GBA3 antibody [008] - BSA and Azide free (AB276056)

Intracellular flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labelling GBA3 with ab276056,followed by a FITC-conjugated secondary antibody (black line) compared with a negative control (grey line). The cells were treated according to manufacturer's manual. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

008

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC

applications

Immunogen

Recombinant Fragment Protein within Human GBA3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H227

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/25 - 1/100", "FlowCytIntra-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GBA3 also known as cytosolic beta-glucosidase is an enzyme with a molecular mass of approximately 60 kDa. This protein functions to hydrolyze glycosides specifically beta-D-glucosides and other saccharide derivatives facilitating the breakdown of these molecules into useful substrates for the cell. GBA3 is expressed in various tissues such as liver kidney and small intestine indicating its widespread role in metabolism.

Biological function summary

Enzymes like cytosolic beta-glucosidase contribute to essential digestive and metabolic processes by breaking down glycosidic bonds found in complex carbohydrates. They are not part of a larger protein complex but they cooperate with other enzymes to maintain energy production from dietary sources. This activity helps the organism to manage carbohydrate metabolism effectively releasing simple sugars that cells use for energy.

Pathways

GBA3 interacts closely with glycolysis and gluconeogenesis important pathways for energy homeostasis. In these pathways GBA3's role in carbohydrate breakdown supplies simple sugars that enter further stages of energy production. It acts alongside proteins like glucokinase and phosphoglucomutase which also contribute to these metabolic pathways aiding in the management of energy resources in the body.

Abnormal activity or expression of GBA3 can link to metabolic conditions like Gaucher's disease and Niemann-Pick disease. These are lysosomal storage disorders where disruptions in glycoside processing can lead to harmful accumulation of glucosylceramides. Proteins such as glucocerebrosidase (GBA1) are also affected in these disorders highlighting a network where GBA3 activity is vital for preventing substrate buildup and maintaining cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neutral cytosolic beta-glycosidase with a broad substrate specificity that could play a role in the catabolism of glycosylceramides (PubMed : 11389701, PubMed : 11784319, PubMed : 17595169, PubMed : 20728381, PubMed : 26724485, PubMed : 33361282). Has a significant glucosylceramidase activity in vitro (PubMed : 17595169, PubMed : 26724485). However, that activity is relatively low and its significance in vivo is not clear (PubMed : 17595169, PubMed : 20728381, PubMed : 26724485). Hydrolyzes galactosylceramides/GalCers, glucosylsphingosines/GlcSphs and galactosylsphingosines/GalSphs (PubMed : 17595169). However, the in vivo relevance of these activities is unclear (PubMed : 17595169). It can also hydrolyze a broad variety of dietary glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens in vitro and could therefore play a role in the metabolism of xenobiotics (PubMed : 11784319). Possesses transxylosylase activity in vitro using xylosylated ceramides/XylCers (such as beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine) as xylosyl donors and cholesterol as acceptor (PubMed : 33361282). Could also play a role in the catabolism of cytosolic sialyl free N-glycans (PubMed : 26193330).

See full target information GBA3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GBA3 antibody [008] - BSA and Azide free

Immunocytochemistry - Anti-GBA3 antibody [008] - BSA and Azide free (AB276056)

Flow Cytometry (Intracellular) - Anti-GBA3 antibody [008] - BSA and Azide free (AB276056)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise