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AB154897

Anti-GBA3 antibody

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(1 Publication)

Rabbit Polyclonal GBA3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GBA3 aa 1-150.

View Alternative Names

CBG, CBGL1, GBA3, Cytosolic beta-glucosidase, Cytosolic beta-glucosidase-like protein 1, Cytosolic galactosylceramidase, Cytosolic glucosylceramidase, Cytosolic glycosylceramidase, Glucosidase beta acid 3, Glucosylceramidase beta 3, Klotho-related protein, Cytosolic GCase, KLrP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBA3 antibody (AB154897)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBA3 antibody (AB154897)

Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling GBA3 with ab154897 at a 1/250 dilution.

Western blot - Anti-GBA3 antibody (AB154897)
  • WB

Unknown

Western blot - Anti-GBA3 antibody (AB154897)

10% SDS PAGE

All lanes:

Western blot - Anti-GBA3 antibody (ab154897) at 1/500 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GBA3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9H227

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Alternatively Tris-EDTA buffer (pH8.0) may be used.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GBA3 also known as cytosolic beta-glucosidase is an enzyme with a molecular mass of approximately 60 kDa. This protein functions to hydrolyze glycosides specifically beta-D-glucosides and other saccharide derivatives facilitating the breakdown of these molecules into useful substrates for the cell. GBA3 is expressed in various tissues such as liver kidney and small intestine indicating its widespread role in metabolism.
Biological function summary

Enzymes like cytosolic beta-glucosidase contribute to essential digestive and metabolic processes by breaking down glycosidic bonds found in complex carbohydrates. They are not part of a larger protein complex but they cooperate with other enzymes to maintain energy production from dietary sources. This activity helps the organism to manage carbohydrate metabolism effectively releasing simple sugars that cells use for energy.

Pathways

GBA3 interacts closely with glycolysis and gluconeogenesis important pathways for energy homeostasis. In these pathways GBA3's role in carbohydrate breakdown supplies simple sugars that enter further stages of energy production. It acts alongside proteins like glucokinase and phosphoglucomutase which also contribute to these metabolic pathways aiding in the management of energy resources in the body.

Abnormal activity or expression of GBA3 can link to metabolic conditions like Gaucher's disease and Niemann-Pick disease. These are lysosomal storage disorders where disruptions in glycoside processing can lead to harmful accumulation of glucosylceramides. Proteins such as glucocerebrosidase (GBA1) are also affected in these disorders highlighting a network where GBA3 activity is vital for preventing substrate buildup and maintaining cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neutral cytosolic beta-glycosidase with a broad substrate specificity that could play a role in the catabolism of glycosylceramides (PubMed : 11389701, PubMed : 11784319, PubMed : 17595169, PubMed : 20728381, PubMed : 26724485, PubMed : 33361282). Has a significant glucosylceramidase activity in vitro (PubMed : 17595169, PubMed : 26724485). However, that activity is relatively low and its significance in vivo is not clear (PubMed : 17595169, PubMed : 20728381, PubMed : 26724485). Hydrolyzes galactosylceramides/GalCers, glucosylsphingosines/GlcSphs and galactosylsphingosines/GalSphs (PubMed : 17595169). However, the in vivo relevance of these activities is unclear (PubMed : 17595169). It can also hydrolyze a broad variety of dietary glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens in vitro and could therefore play a role in the metabolism of xenobiotics (PubMed : 11784319). Possesses transxylosylase activity in vitro using xylosylated ceramides/XylCers (such as beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine) as xylosyl donors and cholesterol as acceptor (PubMed : 33361282). Could also play a role in the catabolism of cytosolic sialyl free N-glycans (PubMed : 26193330).
See full target information GBA3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 14:e0221344 PubMed31419250

2019

Aeromonas sobria serine protease decreases epithelial barrier function in T84 cells and accelerates bacterial translocation across the T84 monolayer in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Hidetomo Kobayashi,Soshi Seike,Masafumi Yamaguchi,Mitsunobu Ueda,Eizo Takahashi,Keinosuke Okamoto,Hiroyasu Yamanaka
View all publications
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Product promise

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