AB153833

Anti-GBAS antibody

Name: Anti-GBAS antibody (ab153833) | Abcam
Brand: Abcam Limited
SKU: AB153833
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal GBAS antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NIPSNAP2 aa 1-250.

View Alternative Names

GBAS, NIPSNAP2, Protein NipSnap homolog 2, NipSnap2, Glioblastoma-amplified sequence

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody (AB153833)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody (AB153833)

Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue, using ab153833 antibody at 1 : 500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody (AB153833)

Immunohistochemical analysis of paraffin-embedded mouse Muscle, using ab153833 antibody at 1 : 500 dilution.

Unknown

Western blot - Anti-GBAS antibody (AB153833)

12% SDS PAGE
30 µg of whole cell lysate

All lanes:

Western blot - Anti-GBAS antibody (ab153833) at 1/1000 dilution

Lane 1:

A549 cell lysate

Lane 2:

H1299 cell lysate

Lane 3:

HCT116 cell lysate

Predicted band size: 34 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NIPSNAP2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O75323

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GBAS protein also known as Golgi apparatus-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1) is a significant protein in cellular mechanics. GBAS has an approximate molecular weight of 200 kDa. You can find its expression mostly in tissues with high cellular turnover rates such as epithelial tissues and certain types of immune cells. This protein mainly functions within the Golgi apparatus where it plays an important role in regulating vesicular trafficking.

Biological function summary

GBAS contributes to essential cellular processes by participating in the activation of ADP-ribosylation factors (ARFs) as part of a larger protein complex. The protein helps control cargo sorting and lipid composition within the Golgi influencing the secretory pathway. Because of its role in regulating ARFs GBAS is vital in the maintenance of membrane trafficking and cytoskeletal organization impacting cell morphology and function.

Pathways

GBAS is deeply involved in the secretory and endocytic pathways impacting cellular homeostasis. It interacts with proteins like ARF4 and ARF5 ensuring efficient vesicle formation and transport. GBAS acts as a bridge within pathways that manage protein and lipid modifications prior to cell surface delivery or lysosomal degradation.

Research has linked GBAS activity to neurodegenerative disorders and certain cancers given its role in vesicular trafficking. Its dysregulation in neurodegenerative diseases often points to malfunction in ARF-dependent pathways that affect neuron maintenance. In cancers alterations in GBAS function can lead to changes in cellular growth and differentiation frequently involving proteins like ARF1 highlighting its potential as a biomarker or therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein involved in mitophagy by facilitating recruitment of the autophagy machinery required for clearance of damaged mitochondria (PubMed : 30982665). Accumulates on the mitochondria surface in response to mitochondrial depolarization and acts as a 'eat me' signal by recruiting proteins involved in selective autophagy, such as autophagy receptors (CALCOCO2/NDP52, NBR1, SQSTM1/p62, TAX1BP1 and WDFY3/ALFY) and ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2) (PubMed : 30982665).

See full target information NIPSNAP2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature chemical biology 18:1065-1075 PubMed35788181

2022

Targeting colorectal cancer with small-molecule inhibitors of ALDH1B1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiping Feng,Marisa E Hom,Thomas E Bearrood,Zachary C Rosenthal,Daniel Fernández,Alison E Ondrus,Yuchao Gu,Aaron K McCormick,Madeline G Tomaske,Cody R Marshall,Toni Kline,Che-Hong Chen,Daria Mochly-Rosen,Calvin J Kuo,James K Chen

Molecular medicine reports 24: PubMed34036378

2021

Knockdown of GBAS regulates esophageal cancer cell viability and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Peng,Ke Ma,Hao Rong,Bo Xiao,Jiang Zhu,Jin-Tao He

Developmental cell 49:509-525.e12 PubMed30982665

2019

NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yakubu Princely Abudu,Serhiy Pankiv,Benan John Mathai,Alf Håkon Lystad,Christian Bindesbøll,Hanne Britt Brenne,Matthew Yoke Wui Ng,Bernd Thiede,Ai Yamamoto,Thaddaeus Mutugi Nthiga,Trond Lamark,Camila V Esguerra,Terje Johansen,Anne Simonsen

Investigative ophthalmology & visual science 58:5930-5940 PubMed29164236

2017

Posttranscriptional Regulation of LOXL1 Expression Via Alternative Splicing and Nonsense-Mediated mRNA Decay as an Adaptive Stress Response.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Berner,Matthias Zenkel,Francesca Pasutto,Ursula Hoja,Panah Liravi,Gabriele C Gusek-Schneider,Friedrich E Kruse,Johannes Schödel,Andre Reis,Ursula Schlötzer-Schrehardt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GBAS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody (AB153833)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody (AB153833)

Western blot - Anti-GBAS antibody (AB153833)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise