Rabbit Polyclonal GBAS antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NIPSNAP2 aa 1-250.
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Publications
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- Molecular medicine reports 24:2021Knockdown of GBAS regulates esophageal cancer cell viability and apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Peng,Ke Ma,Hao Rong,Bo Xiao,Jiang Zhu,Jin-Tao HePubMed 34036378
- Developmental cell 49:509-525.e122019NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYakubu Princely Abudu et. al.PubMed 30982665
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NIPSNAP2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link O75323
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Protein involved in mitophagy by facilitating recruitment of the autophagy machinery required for clearance of damaged mitochondria (PubMed:30982665). Accumulates on the mitochondria surface in response to mitochondrial depolarization and acts as a 'eat me' signal by recruiting proteins involved in selective autophagy, such as autophagy receptors (CALCOCO2/NDP52, NBR1, SQSTM1/p62, TAX1BP1 and WDFY3/ALFY) and ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2) (PubMed:30982665).
Alternative names
GBAS, NIPSNAP2, Protein NipSnap homolog 2, NipSnap2, Glioblastoma-amplified sequence
Recommended products
Rabbit Polyclonal GBAS antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NIPSNAP2 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NIPSNAP2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link O75323
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The GBAS protein also known as Golgi apparatus-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1) is a significant protein in cellular mechanics. GBAS has an approximate molecular weight of 200 kDa. You can find its expression mostly in tissues with high cellular turnover rates such as epithelial tissues and certain types of immune cells. This protein mainly functions within the Golgi apparatus where it plays an important role in regulating vesicular trafficking.
- Biological function summary
GBAS contributes to essential cellular processes by participating in the activation of ADP-ribosylation factors (ARFs) as part of a larger protein complex. The protein helps control cargo sorting and lipid composition within the Golgi influencing the secretory pathway. Because of its role in regulating ARFs GBAS is vital in the maintenance of membrane trafficking and cytoskeletal organization impacting cell morphology and function.
- Pathways
GBAS is deeply involved in the secretory and endocytic pathways impacting cellular homeostasis. It interacts with proteins like ARF4 and ARF5 ensuring efficient vesicle formation and transport. GBAS acts as a bridge within pathways that manage protein and lipid modifications prior to cell surface delivery or lysosomal degradation.
- Associated diseases and disorders
Research has linked GBAS activity to neurodegenerative disorders and certain cancers given its role in vesicular trafficking. Its dysregulation in neurodegenerative diseases often points to malfunction in ARF-dependent pathways that affect neuron maintenance. In cancers alterations in GBAS function can lead to changes in cellular growth and differentiation frequently involving proteins like ARF1 highlighting its potential as a biomarker or therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-GBAS antibody (ab153833)
12% SDS PAGE
30 µg of whole cell lysateAll lanes: Western blot - Anti-GBAS antibody (ab153833) at 1/1000 dilution
Lane 1: A549 cell lysate
Lane 2: H1299 cell lysate
Lane 3: HCT116 cell lysate
Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody (ab153833)
Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue, using ab153833 antibody at 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody (ab153833)
Immunohistochemical analysis of paraffin-embedded mouse Muscle, using ab153833 antibody at 1:500 dilution.
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