Anti-GBAS antibody [OTI1B8] (ab139357)

Mouse Monoclonal GBAS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Dog, African green monkey, Recombinant fragment - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NIPSNAP2.

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Images

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357), expandable thumbnail

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Full Length Protein corresponding to Human NIPSNAP2. Database link O75323

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF
Human	Tested	Tested	Tested
Mouse	Tested	Expected	Expected
Rat	Tested	Expected	Expected
African green monkey	Tested	Expected	Expected
Dog	Tested	Expected	Expected
Recombinant fragment - Human	Tested	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/200.00000 - 1/2000.00000	Notes -
Species Rat	Dilution info 1/200.00000 - 1/2000.00000	Notes -
Species Dog	Dilution info 1/200.00000 - 1/2000.00000	Notes -
Species African green monkey	Dilution info 1/200.00000 - 1/2000.00000	Notes -
Species Recombinant fragment - Human	Dilution info 1/200.00000 - 1/2000.00000	Notes -
Species Human	Dilution info 1/200.00000 - 1/2000.00000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/150	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Dog, African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info 1/50.00000 - 1/100.00000	Notes -
Species Human	Dilution info 1/50.00000 - 1/100.00000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Dog, African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information NIPSNAP2

Function

Protein involved in mitophagy by facilitating recruitment of the autophagy machinery required for clearance of damaged mitochondria (PubMed:30982665). Accumulates on the mitochondria surface in response to mitochondrial depolarization and acts as a 'eat me' signal by recruiting proteins involved in selective autophagy, such as autophagy receptors (CALCOCO2/NDP52, NBR1, SQSTM1/p62, TAX1BP1 and WDFY3/ALFY) and ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2) (PubMed:30982665).

Alternative names

GBAS, NIPSNAP2, Protein NipSnap homolog 2, NipSnap2, Glioblastoma-amplified sequence

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Full Length Protein corresponding to Human NIPSNAP2. Database link O75323
Clone number: OTI1B8
Purification technique: Affinity purification
Concentration
Purification notes: Purified from cell culture supernatant by affinity chromatography.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Clone OTI1B8 (formerly 1B8).

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The GBAS protein also known as Golgi apparatus-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1) is a significant protein in cellular mechanics. GBAS has an approximate molecular weight of 200 kDa. You can find its expression mostly in tissues with high cellular turnover rates such as epithelial tissues and certain types of immune cells. This protein mainly functions within the Golgi apparatus where it plays an important role in regulating vesicular trafficking.

Biological function summary

GBAS contributes to essential cellular processes by participating in the activation of ADP-ribosylation factors (ARFs) as part of a larger protein complex. The protein helps control cargo sorting and lipid composition within the Golgi influencing the secretory pathway. Because of its role in regulating ARFs GBAS is vital in the maintenance of membrane trafficking and cytoskeletal organization impacting cell morphology and function.

Pathways

GBAS is deeply involved in the secretory and endocytic pathways impacting cellular homeostasis. It interacts with proteins like ARF4 and ARF5 ensuring efficient vesicle formation and transport. GBAS acts as a bridge within pathways that manage protein and lipid modifications prior to cell surface delivery or lysosomal degradation.

Associated diseases and disorders

Research has linked GBAS activity to neurodegenerative disorders and certain cancers given its role in vesicular trafficking. Its dysregulation in neurodegenerative diseases often points to malfunction in ARF-dependent pathways that affect neuron maintenance. In cancers alterations in GBAS function can lead to changes in cellular growth and differentiation frequently involving proteins like ARF1 highlighting its potential as a biomarker or therapeutic target.

Product promise

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9 product images

Western blot - Anti-GBAS antibody [OTI1B8] (ab139357)
All lanes: Western blot - Anti-GBAS antibody [OTI1B8] (ab139357) at 1/200 dilution
Lane 1: Human testis extract at 10 µg
Lane 2: Human uterus extract at 10 µg
Lane 3: Human breast extract at 10 µg
Lane 4: Human brain extract at 10 µg
Lane 5: Human liver extract at 10 µg
Lane 6: Human ovary extract at 10 µg
Lane 7: Human thyroid gland extract at 10 µg
Lane 8: Human colon extract at 10 µg
Predicted band size: 34 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (ab139357)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GBAS (green) using ab139357 at 1/100 dilution in ICC/IF.
Western blot - Anti-GBAS antibody [OTI1B8] (ab139357)
All lanes: Western blot - Anti-GBAS antibody [OTI1B8] (ab139357) at 1/200 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with the pCMV6-ENTRY control cDNA at 5 µg
Lane 2: HEK-293T cell lysate transfected with pCMV6-ENTRY GBAS cDNA at 5 µg
Predicted band size: 34 kDa
Western blot - Anti-GBAS antibody [OTI1B8] (ab139357)
All lanes: Western blot - Anti-GBAS antibody [OTI1B8] (ab139357) at 1/200 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg
Lane 3: SVT2 cell extract at 35 µg
Lane 4: A549 (human lung carcinoma cell line) cell extract at 35 µg
Lane 5: COS7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg
Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg
Lane 7: MDCK (canine kidney cell line) cell extract at 35 µg
Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg
Lane 9: MCF7 (human breast adenocarcinoma cell line) cell extract at 35 µg
Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (ab139357)
Paraffin-embedded human colon adenocarcinoma tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (ab139357)
Paraffin-embedded human kidney tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (ab139357)
Paraffin-embedded human ovary adenocarcinoma tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (ab139357)
Paraffin-embedded human endometrium adenocarcinoma tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (ab139357)
COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY GBAS stained for GBAS (green) using ab139357 at 1/50 dilution in ICC/IF.