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AB139357

Anti-GBAS antibody [OTI1B8]

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(1 Publication)

Mouse Monoclonal GBAS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Dog, African green monkey, Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NIPSNAP2.

View Alternative Names

GBAS, NIPSNAP2, Protein NipSnap homolog 2, NipSnap2, Glioblastoma-amplified sequence

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (AB139357)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (AB139357)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GBAS (green) using ab139357 at 1/100 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Paraffin-embedded human colon adenocarcinoma tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Paraffin-embedded human ovary adenocarcinoma tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Paraffin-embedded human kidney tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (AB139357)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (AB139357)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY GBAS stained for GBAS (green) using ab139357 at 1/50 dilution in ICC/IF.

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)
  • WB

Unknown

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)

All lanes:

Western blot - Anti-GBAS antibody [OTI1B8] (ab139357) at 1/200 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with the pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY GBAS cDNA at 5 µg

Predicted band size: 34 kDa

false

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)
  • WB

Supplier Data

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)

All lanes:

Western blot - Anti-GBAS antibody [OTI1B8] (ab139357) at 1/200 dilution

Lane 1:

Human testis extract at 10 µg

Lane 2:

Human uterus extract at 10 µg

Lane 3:

Human breast extract at 10 µg

Lane 4:

Human brain extract at 10 µg

Lane 5:

Human liver extract at 10 µg

Lane 6:

Human ovary extract at 10 µg

Lane 7:

Human thyroid gland extract at 10 µg

Lane 8:

Human colon extract at 10 µg

Predicted band size: 34 kDa

false

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)
  • WB

Unknown

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)

All lanes:

Western blot - Anti-GBAS antibody [OTI1B8] (ab139357) at 1/200 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 34 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1B8

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Dog, Human, African green monkey

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NIPSNAP2.

O75323

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Clone OTI1B8 (formerly 1B8).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant by affinity chromatography.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GBAS protein also known as Golgi apparatus-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1) is a significant protein in cellular mechanics. GBAS has an approximate molecular weight of 200 kDa. You can find its expression mostly in tissues with high cellular turnover rates such as epithelial tissues and certain types of immune cells. This protein mainly functions within the Golgi apparatus where it plays an important role in regulating vesicular trafficking.
Biological function summary

GBAS contributes to essential cellular processes by participating in the activation of ADP-ribosylation factors (ARFs) as part of a larger protein complex. The protein helps control cargo sorting and lipid composition within the Golgi influencing the secretory pathway. Because of its role in regulating ARFs GBAS is vital in the maintenance of membrane trafficking and cytoskeletal organization impacting cell morphology and function.

Pathways

GBAS is deeply involved in the secretory and endocytic pathways impacting cellular homeostasis. It interacts with proteins like ARF4 and ARF5 ensuring efficient vesicle formation and transport. GBAS acts as a bridge within pathways that manage protein and lipid modifications prior to cell surface delivery or lysosomal degradation.

Research has linked GBAS activity to neurodegenerative disorders and certain cancers given its role in vesicular trafficking. Its dysregulation in neurodegenerative diseases often points to malfunction in ARF-dependent pathways that affect neuron maintenance. In cancers alterations in GBAS function can lead to changes in cellular growth and differentiation frequently involving proteins like ARF1 highlighting its potential as a biomarker or therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein involved in mitophagy by facilitating recruitment of the autophagy machinery required for clearance of damaged mitochondria (PubMed : 30982665). Accumulates on the mitochondria surface in response to mitochondrial depolarization and acts as a 'eat me' signal by recruiting proteins involved in selective autophagy, such as autophagy receptors (CALCOCO2/NDP52, NBR1, SQSTM1/p62, TAX1BP1 and WDFY3/ALFY) and ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2) (PubMed : 30982665).
See full target information NIPSNAP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5332 PubMed37658083

2023

Atezolizumab plus stereotactic ablative radiotherapy for medically inoperable patients with early-stage non-small cell lung cancer: a multi-institutional phase I trial.

Applications

Unspecified application

Species

Unspecified reactive species

Arta M Monjazeb,Megan E Daly,Guillaume Luxardi,Emanual Maverakis,Alexander A Merleev,Alina I Marusina,Alexander Borowsky,Amin Mirhadi,Stephen L Shiao,Laurel Beckett,Shuai Chen,David Eastham,Tianhong Li,Logan V Vick,Heather M McGee,Frances Lara,Leslie Garcia,Leigh Anne Morris,Robert J Canter,Jonathan W Riess,Kurt A Schalper,William J Murphy,Karen Kelly
View all publications
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