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AB284830

Anti-GBE1 antibody [EP11113] - BSA and Azide free

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Rabbit Monoclonal GBE1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Brancher enzyme, Glycogen-branching enzyme, GBE1

2 Images
Western blot - Anti-GBE1 antibody [EP11113] - BSA and Azide free (AB284830)
  • WB

Supplier Data

Western blot - Anti-GBE1 antibody [EP11113] - BSA and Azide free (AB284830)

This data was developed using ab180596, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GBE1 antibody [EP11113] (<a href='/en-us/products/primary-antibodies/gbe1-antibody-ep11113-ab180596'>ab180596</a>) at 1/2000 dilution

All lanes:

Human skeletal muscle lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 76 kDa,80 kDa

false

Western blot - Anti-GBE1 antibody [EP11113] - BSA and Azide free (AB284830)
  • WB

Supplier Data

Western blot - Anti-GBE1 antibody [EP11113] - BSA and Azide free (AB284830)

This data was developed using ab180596, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GBE1 antibody [EP11113] (<a href='/en-us/products/primary-antibodies/gbe1-antibody-ep11113-ab180596'>ab180596</a>) at 1/10000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

PC-3 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution

Predicted band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP11113

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284830 is the carrier-free version of ab180596

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GBE1 protein also known as glycogen branching enzyme 1 plays an important role in glycogen metabolism. It has a molecular mass of approximately 83 kDa. The enzyme catalyzes the formation of alpha-16-glycosidic linkages in glycogen adding branches to the linear chains of glucose residues. This branching increases the solubility of glycogen and improves its accessibility for degradation. GBE1 expression occurs mainly in the liver and muscle tissues where glycogen storage and metabolism are vital for energy balance and function.
Biological function summary

The enzyme's activity ensures the efficient storage and mobilization of glycogen serving an important role in energy regulation. It does not operate as part of a larger enzymatic complex but works alone to modify the structure of glycogen. Its activity impacts the balance between corticosteroid glucose production and glycogen storage thereby playing a part in maintaining glucose homeostasis in the body.

Pathways

GBE1 participates in the glycogen biosynthesis pathway which is vital for energy storage in organisms. The protein acts alongside others like glycogen synthase to ensure proper glycogen formation which serves as a significant energy reserve. Also GBE1 relates to the gluconeogenesis/glycogenolysis pathway where its role in structuring glycogen ensures substrates are readily available when needed for energy production during fasting or rigorous activity.

Mutations or malfunctions in the GBE1 gene can lead to Glycogen Storage Disease type IV (Andersen's disease) a disorder affecting glycogen metabolism. Symptoms can include hepatic and muscular dysfunctions due to improperly structured glycogen. The condition is associated with another protein glycogen synthase because both contribute to abnormal glycogen accumulation. Also research links enzyme deficits to adult polyglucosan body disease where abnormal glycogen bodies accumulate in the nervous system leading to neurological issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycogen-branching enzyme participates in the glycogen biosynthetic process along with glycogenin and glycogen synthase. Generates alpha-1,6-glucosidic branches from alpha-1,4-linked glucose chains, to increase solubility of the glycogen polymer (PubMed : 26199317, PubMed : 8463281, PubMed : 8613547).
See full target information 1,4-alpha-glucan-branching enzyme
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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