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AB250813

Anti-Gbeta5 antibody [EPR14779(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Gbeta5 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Guanine nucleotide-binding protein subunit beta-5, Gbeta5, Transducin beta chain 5, GNB5

2 Images
Western blot - Anti-Gbeta5 antibody [EPR14779(B)] - BSA and Azide free (AB250813)
  • WB

Supplier Data

Western blot - Anti-Gbeta5 antibody [EPR14779(B)] - BSA and Azide free (AB250813)

This data was developed using ab185214, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Gbeta5 antibody [EPR14779(B)] (<a href='/en-us/products/primary-antibodies/gbeta5-antibody-epr14779b-ab185214'>ab185214</a>) at 1/1000 dilution

All lanes:

Human cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 39 kDa

false

Western blot - Anti-Gbeta5 antibody [EPR14779(B)] - BSA and Azide free (AB250813)
  • WB

Supplier Data

Western blot - Anti-Gbeta5 antibody [EPR14779(B)] - BSA and Azide free (AB250813)

This data was developed using ab185214, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Gbeta5 antibody [EPR14779(B)] (<a href='/en-us/products/primary-antibodies/gbeta5-antibody-epr14779b-ab185214'>ab185214</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14779(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250813 is the carrier-free version of ab185214.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gbeta5 also known as Guanine nucleotide-binding protein subunit beta-5 is a regulatory component of heterotrimeric G-proteins. It has an approximate mass of 37 kDa. Gbeta5 is expressed in multiple tissues with significant levels found in the brain and retina. Mechanically Gbeta5 acts as a modulator for signal transduction pathways partnering with the regulator of G-protein signaling (RGS) proteins to influence neuronal signaling processes.
Biological function summary

Gbeta5 functions as an integral part of the G-protein coupled receptor (GPCR) signaling system. It forms stable complexes with RGS proteins particularly RGS6 RGS7 RGS9 and RGS11. These complexes regulate the deactivation of G-proteins impacting the timing and efficacy of signaling events. The Gbeta5 and RGS complexes contribute to the modulation of neurotransmitter release and synaptic plasticity critical in nervous system functionalities.

Pathways

Gbeta5 plays a significant role in neurotransmission pathways such as the dopaminergic and serotonergic pathways. Its activity is linked to the fine-tuning of neuronal signals by influencing cyclic AMP levels and Ca2+ channel activity. Gbeta5 interacts with proteins like RGS9 which is important in visual and dopaminergic signaling highlighting its importance in these specific pathways.

Mutations or dysregulation in Gbeta5 have associations with neurodegenerative conditions and mood disorders such as Alzheimer's disease and major depressive disorder. The dysregulated activity can lead to altered interaction with proteins like RGS7 and RGS9 affecting normal neuronal signaling and function which may contribute to the pathology of these conditions. Understanding Gbeta5’s role can provide insights into developing therapeutic strategies for these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, such as RGS7 and RGS9, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (PubMed : 27677260). Increases RGS7 GTPase-activating protein (GAP) activity, thereby regulating mood and cognition (By similarity). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D(2) dopamine receptors (PubMed : 27677260). May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate (By similarity).
See full target information Guanine nucleotide-binding protein subunit beta-5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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