AB86071

Anti-GBF1 antibody

Name: Anti-GBF1 antibody (ab86071) | Abcam
Brand: Abcam Limited
SKU: AB86071
Rating: 5 (4 reviews)

(4 Reviews)

(17 Publications)

Rabbit Polyclonal GBF1 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human, Mouse samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human GBF1 aa 1800 to C-terminus.

View Alternative Names

KIAA0248, GBF1, Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1, BFA-resistant GEF 1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-GBF1 antibody (AB86071)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-GBF1 antibody (AB86071)

ab86071 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86071 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 antibody (AB86071)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 antibody (AB86071)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling GBF1 with ab86071 at 1/200 (1µg/ml). Detection : DAB.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GBF1 antibody (AB86071)

ab86071 staining GBF1 in HeLa cells treated with Exo-1 (ab120292), by ICC/IF. Increase in GBF1 expression correlates with increased concentration of Exo-1 as described in literature.
The cells were incubated at 37°C for 5 minutes in media containing different concentrations of ab120292 ( Exo-1) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab86071 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Unknown

Immunoprecipitation - Anti-GBF1 antibody (AB86071)

Immunoprecipitation/ Western Blot of ab86071. Lane 1 : ab86071 at 3µg/mg whole cell lysate. Lane 2 : Control IgG. ab86071 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-GBF1 antibody (ab86071)

Predicted band size: 206 kDa

false

Unknown

Western blot - Anti-GBF1 antibody (AB86071)

All lanes:

Western blot - Anti-GBF1 antibody (ab86071) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T cell lysate at 50 µg

Lane 5:

mouse NIH3T3 cell lysate at 50 µg

Predicted band size: 206 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human GBF1 aa 1800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92538

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Golgi Brefeldin A-Resistant Guanine Nucleotide Exchange Factor 1 (GBF1) also known by the alternate name ARFGEF1 is a protein that acts mechanically as a guanine nucleotide exchange factor (GEF) for ADP-ribosylation factor (ARF) proteins facilitating the conversion of ARF-GDP to ARF-GTP. This protein has a molecular mass of approximately 206 kDa. GBF1 is mainly expressed in the cytoplasm and localizes to the Golgi apparatus which is a central organelle for protein and lipid transport.

Biological function summary

GBF1 plays a significant role in the regulation of vesicular trafficking between the endoplasmic reticulum and the Golgi apparatus. It is an integral component of the Secretory Pathway where it aids in the recruitment of coat protein complex I (COPI) to budding vesicles. As part of a larger protein complex GBF1 collaborates with ARF proteins to activate the pathways necessary for maintaining Golgi structure and function.

Pathways

GBF1 contributes directly to the Arf protein signal transduction pathway playing a role in maintaining the Golgi structure necessary for cellular transport processes. The protein interacts with several partners within this pathway including large GTPase SAR1 and small GTPase ARF1 essential for vesicle formation. GBF1's involvement in these pathways ensures proper intracellular transport and membrane trafficking activities which are important for cell viability.

GBF1 has shown a connection to disorders such as Alzheimer's disease due to its key role in intracellular trafficking which is important for amyloid precursor protein processing. Disruption in GBF1 function can lead to altered protein and lipid homeostasis linking it to metabolic disorders as well. The protein also interacts with other proteins like ARF3 in pathological conditions and disruptions in these interactions can have significant implications in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum (ER) (PubMed : 12047556, PubMed : 12808027, PubMed : 16926190, PubMed : 17956946, PubMed : 18003980, PubMed : 19039328, PubMed : 24213530). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adaptor protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5) (PubMed : 23386609). Has GEF activity towards ARF1 (PubMed : 15616190). Has in vitro GEF activity towards ARF5 (By similarity). Involved in the processing of PSAP (PubMed : 17666033). Required for the assembly of the Golgi apparatus (PubMed : 12808027, PubMed : 18003980). The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions (PubMed : 18063581, PubMed : 23418352). May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate (PubMed : 19461073, PubMed : 22185782). In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex (PubMed : 22573891). Plays a role in maintaining mitochondrial morphology (PubMed : 25190516).

See full target information GBF1

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 99:e0096225 PubMed40838750

2025

Coatomer protein complex I is required for efficient secretion of dengue virus non-structural protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen M Johnson,Siena M Centofanti,Gustavo Bracho,Michael R Beard,Jillian M Carr,Nicholas S Eyre

Cell communication and signaling : CCS 21:156 PubMed37370099

2023

The crescent-like Golgi ribbon is shaped by the Ajuba/PRMT5/Aurora-A complex-modified HURP.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Chih Chiu,Xin-Ting Yang,Tong-You Wade Wei,Yu-Ting Amber Liao,Jo-Mei Maureen Chen,Yi-Chun Kuo,Chun-Chih Jared Liu,Chiao-Yun Cheng,Yu-Ting Jenny Huang,Yun-Ru Jaoying Huang,He-Lian Joe Wu,Chang-Xin Wan,Jia-Rung Tsai,Chang-Tze Ricky Yu

Biology open 12: PubMed36946871

2023

Kinetics of Arf1 inactivation regulates Golgi organisation and function in non-adherent fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Rajeshwari B R,Nikita Shah,Prachi Joshi,M S Madhusudan,Nagaraj Balasubramanian

The Journal of cell biology 221: PubMed36305789

2022

Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.

Applications

Unspecified application

Species

Unspecified reactive species

Arti T Navare,Fred D Mast,Jean Paul Olivier,Thierry Bertomeu,Maxwell L Neal,Lindsay N Carpp,Alexis Kaushansky,Jasmin Coulombe-Huntington,Mike Tyers,John D Aitchison

Communications biology 4:1370 PubMed34876695

2021

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Neeraj Tiwari,Lianhui Zhu,Hai Dang Truong Ngo,Jae-Min Lim,Bopil Gim,Shuaiyang Jing,Yijing Wang,Yi Qian,Intaek Lee

BMC biology 19:194 PubMed34493279

2021

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Yi Qian,Lianhui Zhu,Bopil Gim,Mengjing Bao,Jie Jia,Shuaiyang Jing,Yijing Wang,Chuanting Tan,Francesca Bottanelli,Pascal Ziltener,Sunkyu Choi,Piliang Hao,Intaek Lee

bioRxiv : the preprint server for biology 221: PubMed33173868

2020

Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.

Applications

Unspecified application

Species

Unspecified reactive species

Arti T Navare,Fred D Mast,Jean Paul Olivier,Thierry Bertomeu,Maxwell Neal,Lindsay N Carpp,Alexis Kaushansky,Jasmin Coulombe-Huntington,Mike Tyers,John D Aitchison

Veterinary microbiology 246:108743 PubMed32605744

2020

Rab1b-GBF1-ARFs mediated intracellular trafficking is required for classical swine fever virus replication in swine umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhang,Tao Wang,Mengzhao Song,Mingxing Jin,Shanchuan Liu,Kangkang Guo,Yanming Zhang

Molecular cell 77:748-760.e9 PubMed31785928

2019

Immunosuppression by Mutated Calreticulin Released from Malignant Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Liu,Liwei Zhao,Friedemann Loos,Caroline Marty,Wei Xie,Isabelle Martins,Sylvie Lachkar,Bo Qu,Emmanuelle Waeckel-Énée,Isabelle Plo,William Vainchenker,Franck Perez,David Rodriguez,Carlos López-Otin,Peter van Endert,Laurence Zitvogel,Oliver Kepp,Guido Kroemer

Scientific reports 8:14966 PubMed30297865

2018

Identification of pharmacological inhibitors of conventional protein secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei Zhao,Peng Liu,Gaelle Boncompain,Friedemann Loos,Sylvie Lachkar,Lucillia Bezu,Guo Chen,Heng Zhou,Franck Perez,Oliver Kepp,Guido Kroemer

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Anti-GBF1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GBF1 antibody (AB86071)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBF1 antibody (AB86071)

Immunocytochemistry/ Immunofluorescence - Anti-GBF1 antibody (AB86071)

Immunoprecipitation - Anti-GBF1 antibody (AB86071)

Western blot - Anti-GBF1 antibody (AB86071)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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