Anti-GBP2 antibody [EPR13206] - BSA and Azide free

• WB

Lab

Western blot - Anti-GBP2 antibody [EPR13206] - BSA and Azide free (AB250128)

This data was developed using ab179829, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab179829 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.

ab179829 Anti-GBP2 antibody [EPR13206] - N-terminal was shown to specifically react with GBP2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267218 (knockout cell lysate ab257962) was used. Wild-type and GBP2 knockout samples were subjected to SDS-PAGE. ab179829 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (<a href='/en-us/products/primary-antibodies/gbp2-antibody-epr13206-n-terminal-ab179829'>ab179829</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

GBP2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human GBP2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-gbp2-knockout-a549-cell-line-ab267218'>ab267218</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate at 20 µg

Lane 4:

HaCaT (Human keratinocyte cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

• WB

Lab

Western blot - Anti-GBP2 antibody [EPR13206] - BSA and Azide free (AB250128)

This data was developed using ab179829, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab179829 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.

ab179829 Anti-GBP2 antibody [EPR13206] - N-terminal was shown to specifically react with GBP2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267219 (knockout cell lysate ab257963) was used. Wild-type and GBP2 knockout samples were subjected to SDS-PAGE. ab179829 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (<a href='/en-us/products/primary-antibodies/gbp2-antibody-epr13206-n-terminal-ab179829'>ab179829</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

GBP2 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human GBP2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-gbp2-knockout-a549-cell-line-ab267219'>ab267219</a>)

Lane 3:

K-562 cell lysate at 20 µg

Lane 4:

HaCaT cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 67 kDa

Observed band size: 70 kDa

false

• WB

Supplier Data

Western blot - Anti-GBP2 antibody [EPR13206] - BSA and Azide free (AB250128)

This data was developed using ab179829, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (<a href='/en-us/products/primary-antibodies/gbp2-antibody-epr13206-n-terminal-ab179829'>ab179829</a>) at 1/1000 dilution

Lane 1:

Human spleen cell lysate

Lane 2:

IM-9 cell lysate

Lane 3:

HACAT cell lysate

Lane 4:

K562 cell lysate

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 67 kDa

false