Rabbit Polyclonal GBP4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanylate-binding protein 4 aa 1-300.
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- Journal of cancer research and clinical oncology 148:2673-26802021Protein expression of prognostic genes in primary melanoma and benign nevi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesT Gambichler,J Elfering,T Meyer,S Bruckmüller,E Stockfleth,M Skrygan,H U Käfferlein,T Brüning,K Lang,D Wagener,S Schröder,M Nick,L SusokPubMed 34757537
- Frontiers in genetics 12:6804132021Identification of a Prognostic Signature for Ovarian Cancer Based on the Microenvironment Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Huo,Hengzi Sun,Shuangwu Liu,Bing Liang,Huimin Bai,Shuzhen Wang,Shuhong LiPubMed 34054929
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Guanylate-binding protein 4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PP9
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens (By similarity). Negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor (By similarity).
Alternative names
Guanylate-binding protein 4, GTP-binding protein 4, Guanine nucleotide-binding protein 4, GBP-4, GBP4
Recommended products
Rabbit Polyclonal GBP4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Guanylate-binding protein 4 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Guanylate-binding protein 4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PP9
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GBP4 also known as guanylate-binding protein 4 is a member of the guanylate-binding protein family. This family is known for its role in hydrolyzing GTP to GDP and GMP an important mechanical activity that GBP4 performs. GBP4 has a mass of approximately 68 kDa. The protein is expressed in a variety of tissues but it shows higher expression in immune-related cells. It is often upregulated in response to interferon-gamma a cytokine important for immune response modulation.
- Biological function summary
GBP4 plays an important role in immune response against pathogens. By disrupting microbial membranes GBP4 aids in restricting the spread of certain intracellular bacteria. It may also participate in forming a larger complex with other GBP proteins for more efficient pathogen targeting. This action is particularly significant during infections where increased GBP4 expression provides a stronger defense mechanism as part of the innate immune system.
- Pathways
GBP4 integrates functionally into interferon-gamma and JAK-STAT signaling pathways. These pathways are key components of the immune response to infections and inflammation. GBP4's role in these pathways highlights its interaction with other immune proteins such as STAT1 and other guanylate-binding proteins. These interactions highlight GBP4's importance in signaling cascades that strategize cellular immune defenses.
- Associated diseases and disorders
GBP4 exhibits links to conditions like autoimmune diseases and certain infections. In autoimmune pathologies dysregulation involving GBP4 may contribute to inappropriate immune activation potentially related to proteins such as interferon-gamma. Additionally in infections GBP4 plays a role in controlling bacterial infections within macrophages often in cooperation with other members of the guanylate-binding protein family. Understanding these connections provides insights into therapeutic interventions targeting immune pathways in disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-GBP4 antibody (ab232693)
All lanes: Western blot - Anti-GBP4 antibody (ab232693) at 2 µg/mL
All lanes: Recombinant human GBP4 protein.
Predicted band size: 73 kDa
Western blot - Anti-GBP4 antibody (ab232693)
All lanes: Western blot - Anti-GBP4 antibody (ab232693) at 2 µg/mL
All lanes: A431 (human epidermoid carcinoma cell line) cell lysate.
Predicted band size: 73 kDa
Western blot - Anti-GBP4 antibody (ab232693)
All lanes: Western blot - Anti-GBP4 antibody (ab232693) at 2 µg/mL
All lanes: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate.
Predicted band size: 73 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP4 antibody (ab232693)
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for GBP4 using ab232693 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP4 antibody (ab232693)
Formalin-fixed, paraffin-embedded human kidney tissue stained for GBP4 using ab232693 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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