JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB70058

Anti-GBP4 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Polyclonal GBP4 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Guanylate-binding protein 4.

View Alternative Names

Guanylate-binding protein 4, GTP-binding protein 4, Guanine nucleotide-binding protein 4, GBP-4, GBP4

2 Images
Western blot - Anti-GBP4 antibody (AB70058)
  • WB

Unknown

Western blot - Anti-GBP4 antibody (AB70058)

All lanes:

Western blot - Anti-GBP4 antibody (ab70058) at 1/500 dilution

Lane 1:

GBP4 transfected 293T lysate at 25 µg

Lane 2:

Non-transfected 293T lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 73 kDa

Observed band size: 74 kDa

false

Western blot - Anti-GBP4 antibody (AB70058)
  • WB

Unknown

Western blot - Anti-GBP4 antibody (AB70058)

All lanes:

Western blot - Anti-GBP4 antibody (ab70058) at 1/500 dilution

All lanes:

human pancreas tissue lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 73 kDa

Observed band size: 74 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Guanylate-binding protein 4.

Q96PP9

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GBP4 also known as guanylate-binding protein 4 is a member of the guanylate-binding protein family. This family is known for its role in hydrolyzing GTP to GDP and GMP an important mechanical activity that GBP4 performs. GBP4 has a mass of approximately 68 kDa. The protein is expressed in a variety of tissues but it shows higher expression in immune-related cells. It is often upregulated in response to interferon-gamma a cytokine important for immune response modulation.
Biological function summary

GBP4 plays an important role in immune response against pathogens. By disrupting microbial membranes GBP4 aids in restricting the spread of certain intracellular bacteria. It may also participate in forming a larger complex with other GBP proteins for more efficient pathogen targeting. This action is particularly significant during infections where increased GBP4 expression provides a stronger defense mechanism as part of the innate immune system.

Pathways

GBP4 integrates functionally into interferon-gamma and JAK-STAT signaling pathways. These pathways are key components of the immune response to infections and inflammation. GBP4's role in these pathways highlights its interaction with other immune proteins such as STAT1 and other guanylate-binding proteins. These interactions highlight GBP4's importance in signaling cascades that strategize cellular immune defenses.

GBP4 exhibits links to conditions like autoimmune diseases and certain infections. In autoimmune pathologies dysregulation involving GBP4 may contribute to inappropriate immune activation potentially related to proteins such as interferon-gamma. Additionally in infections GBP4 plays a role in controlling bacterial infections within macrophages often in cooperation with other members of the guanylate-binding protein family. Understanding these connections provides insights into therapeutic interventions targeting immune pathways in disease management.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens (By similarity). Negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor (By similarity).
See full target information Guanylate-binding protein 4
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Technology in cancer research & treatment 19:1533033820957006 PubMed33000678

2020

Down-Regulation of AHNAK2 Inhibits Cell Proliferation, Migration and Invasion Through Inactivating the MAPK Pathway in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Wei Wang,Hai-Zheng Zheng,Na Cha,Xiao-Jie Zhang,Min Zheng,Ming-Ming Chen,Li-Xiang Tian
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB232693

Anti-GBP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP4 antibody (AB232693)

  • 4
  • 1
Primary Antibodies

AB232689

Anti-GBP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP4 antibody (AB232689)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB182981

Anti-CD31 antibody [EPR17259]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-CD31 antibody [EPR17259] (AB182981)

  • 5
  • 22
Primary Antibodies

AB140795

Anti-GPR91 antibody - Cytoplasmic domain

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR91 antibody - Cytoplasmic domain (AB140795)

  • 0
  • 0
Primary Antibodies

AB134918

Anti-SERPING1 antibody [EPR8015]

RabMAb|Recombinant

Western blot - Anti-SERPING1 antibody [EPR8015] (AB134918)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com