Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)

Rabbit Recombinant Monoclonal GBP5 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (AB313391), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt (Intra)	WB	ICC/IF	IHC-P	IP
Human	Tested	Tested	Tested	Tested	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended
Rat	Expected	Not recommended	Expected	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information GBP5

Function

Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens (By similarity). Hydrolyzes GTP, but in contrast to other family members, does not produce GMP (PubMed:20180847). Following infection, recruited to the pathogen-containing vacuoles or vacuole-escaped bacteria and acts as a positive regulator of inflammasome assembly by promoting the release of inflammasome ligands from bacteria (By similarity). Acts by promoting lysis of pathogen-containing vacuoles, releasing pathogens into the cytosol (By similarity). Following pathogen release in the cytosol, promotes recruitment of proteins that mediate bacterial cytolysis: this liberates ligands that are detected by inflammasomes, such as lipopolysaccharide (LPS) that activates the non-canonical CASP4/CASP11 inflammasome or double-stranded DNA (dsDNA) that activates the AIM2 inflammasome (By similarity). As an activator of NLRP3 inflammasome assembly: promotes selective NLRP3 inflammasome assembly in response to microbial and soluble, but not crystalline, agents (PubMed:22461501). Independently of its GTPase activity, acts as an inhibitor of various viruses infectivity, such as HIV-1, Zika and influenza A viruses, by inhibiting FURIN-mediated maturation of viral envelope proteins (PubMed:26996307, PubMed:31091448). Antigenic tumor-specific truncated splice form.

Alternative names

UNQ2427/PRO4987, GBP5, Guanylate-binding protein 5, GBP-TA antigen, GTP-binding protein 5, Guanine nucleotide-binding protein 5, GBP-5

Recommended products

Rabbit Recombinant Monoclonal GBP5 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28367-83
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GBP5 or Guanylate Binding Protein 5 serves an important role in the immune response particularly within the innate immune system. GBP5 is a GTPase with a molecular mass of approximately 71 kDa. It expresses in a variety of tissues including immune cells like macrophages and lymphocytes. Its mechanical function involves binding to guanosine triphosphate (GTP) and converting it to guanosine diphosphate (GDP) a process which then triggers further downstream immune responses.

Biological function summary

GBP5 acts in immune defense against intracellular pathogens. It forms part of a multiprotein complex that can directly target and destroy bacterial membranes. GBP5 enhances the cell-autonomous immunity by promoting inflammasome activation which boosts cytokine production leading to a stronger inflammatory response. Its activity reflects in the ability of immune cells to restrict the growth of certain pathogens hence forming an important part of the host defense mechanism.

Pathways

GBP5 is part of the interferon-gamma signaling pathway. This pathway engages in regulating immune responses during infections particularly by modulating the activity of other immune-related proteins such as GBP1 and GBP2. GBP5 links to the NF-kB pathway as well where it helps regulation of inflammatory responses. The efficient functioning of these pathways ensures that immune responses remain robust and effective against pathogens.

Associated diseases and disorders

GBP5 is linked to tuberculosis and Crohn’s disease. Its role in immune responses impacts how efficiently the body can deal with intracellular mycobacterial infections typical of tuberculosis. In Crohn’s disease GBP5 associates with inflammatory processes that drive pathology. Through these conditions GBP5 often interfaces with proteins like NOD2 which shares a similar role in recognizing and responding to microbial components.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

16 product images

Flow Cytometry (Intracellular) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) treated with 1µg/mL LPS for 24h and then 40ng/mL IFN gamma for 24h (Red) / untreated J774A.1 (Green) cells labelling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/500 dilution (0.1 ug)/Red and Green compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of THP-1 (human monocytic leukemia monocyte) treated with 1µg/mL LPS for 24h and then 40ng/mL IFN gamma for 24h (Red) / untreated THP-1 (Green) cells labelling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/500 dilution (0.1 ug)/Red and Green compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) THP-1 (human mon tissue labeling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/2000 (0.254 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) THP-1 treated with 1ug/ml LPS and IFN gamma for 24 hours cell pellet, no staining on (B) Untreated THP-1 cell pellet. The section was incubated with Anti-GBP5 antibody [EPR28367-83] ab313390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The cell induction condition was referenced from literature(PMID:22461501).

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 15 seconds
All lanes: Western blot - Anti-GBP5 antibody [EPR28367-83] (Anti-GBP5 antibody [EPR28367-83] ab313390) at 1/1000 dilution
Lane 1: Untreated J774A.1 (Mouse reticum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg
Lane 2: J774A.1 treated with /ml LPS and IFN gamma for 24 hours, was then added for an additional 300ng/ml BFA for last 20 hours whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 66 kDa
Exposure time: 15s
Western blot - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: brain(PMID: 33608513).

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
All lanes: Western blot - Anti-GBP5 antibody [EPR28367-83] (Anti-GBP5 antibody [EPR28367-83] ab313390) at 1/1000 dilution
Lane 1: Human colon tissue lysate at 20 µg
Lane 2: Human brain tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 66 kDa
Exposure time: 180s
Western blot - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The cell induction condition was referenced from literature(PMID:22461501).

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: Lane1-2: 180 seconds, lane3-4: 15 seconds
All lanes: Western blot - Anti-GBP5 antibody [EPR28367-83] (Anti-GBP5 antibody [EPR28367-83] ab313390) at 1/1000 dilution
Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HeLa treated with /ml IFN alpha for 16 hours whole cell lysate at 20 µg
Lane 3: Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 4: THP-1 treated with /ml LPS and IFN gamma for 24 hours whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 66 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/50 (10.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in J774A.1 cells treated with 1 µg/mL LPS (lipopolysaccharide) and 200 ng/mL IFN gamma (mouse) for 24 hours, while showing no staining in untreated J774A.1 cells.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/50 (10.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cells treated with 1 µg/mL LPS (lipopolysaccharide) and 40 ng/mL IFN gamma for 24 hours, while showing no staining in untreated THP-1 cells.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/2000 (0.254 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression: no staining on rat cerebrum. The section was incubated with Anti-GBP5 antibody [EPR28367-83] ab313390 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/2000 (0.254 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression: no staining on mouse cerebrum. The section was incubated with Anti-GBP5 antibody [EPR28367-83] ab313390 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/4000 (0.127 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression: no staining on human cerebrum (PMID: 33608513). The section was incubated with Anti-GBP5 antibody [EPR28367-83] ab313390 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/2000 (0.254 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells of rat colon. The section was incubated with Anti-GBP5 antibody [EPR28367-83] ab313390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse glioblastoma tissue labeling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/2000 (0.254 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse glioblastoma. The section was incubated with Anti-GBP5 antibody [EPR28367-83] ab313390 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/2000 (0.254 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells of mouse colon. The section was incubated with Anti-GBP5 antibody [EPR28367-83] ab313390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/4000 (0.127 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells of human colon carcinoma. The section was incubated with Anti-GBP5 antibody [EPR28367-83] ab313390 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free (ab313391)
This data was developed using Anti-GBP5 antibody [EPR28367-83] ab313390, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling GBP5 with Anti-GBP5 antibody [EPR28367-83] ab313390 at 1/4000 (0.127 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil. The section was incubated with Anti-GBP5 antibody [EPR28367-83] ab313390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins