JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109726

Anti-Gbx2 antibody

1

(1 Review)

|

(1 Publication)

Goat Polyclonal GBX2 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GBX2 aa 150-200.

View Alternative Names

Homeobox protein GBX-2, Gastrulation and brain-specific homeobox protein 2, GBX2

3 Images
Western blot - Anti-Gbx2 antibody (AB109726)
  • WB

Unknown

Western blot - Anti-Gbx2 antibody (AB109726)

All lanes:

Western blot - Anti-Gbx2 antibody (ab109726) at 0.1 µg/mL

All lanes:

Jurkat cell lysate (in RIPA buffer) at 35 µg

Predicted band size: 37 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbx2 antibody (AB109726)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbx2 antibody (AB109726)

Human Placenta : Formalin-Fixed, Paraffin-Embedded (FFPE) using ab109726 at a concentration of 3.75 µg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbx2 antibody (AB109726)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbx2 antibody (AB109726)

Human Brain, cortex : Formalin-Fixed, Paraffin-Embedded (FFPE) using ab109726 at a concentration of 3.75 µg/ml.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ELISA, IHC-P

applications

Immunogen

Synthetic Peptide within Human GBX2 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P52951

style
left-column

Alternative Products

Primary Antibodies

AB227853

Anti-Gbx2 antibody

Western blot - Anti-Gbx2 antibody (AB227853)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB21990

Anti-Otx1 + Otx2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody (AB21990)

  • 5
  • 11
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB205921

Anti-PD-L1 antibody [28-8]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (AB205921)

  • 4
  • 15
Primary Antibodies

AB64807

Anti-FOXD3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXD3 antibody (AB64807)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gbx2 also known as Gastrulation Brain Homeobox 2 functions as a transcription factor. This protein approximately 24 kDa in mass plays a significant role in gene regulation during embryonic development. Gbx2 is expressed mainly in the developing nervous system particularly in the hindbrain and midbrain regions. It influences the expression of other genes critical for brain and cranial nerve development.
Biological function summary

The role of this transcription factor centers on regulating neural development processes. Gbx2 participates in formation and patterning of the central nervous system. It does not typically operate alone; instead it often interacts with other transcription factors to modulate gene expression. Gbx2 is important for demarcating boundaries in the hindbrain which contributes to proper compartmentalization and formation of rhombomeres.

Pathways

Gbx2 contributes significantly to the Wnt and FGF signaling pathways which are fundamental during early neural development stages. In these pathways Gbx2 interacts closely with other key proteins such as Otx2 another homeobox gene to establish clear anterior-posterior axis in the developing brain. These interactions are essential to ensure proper differentiation and positioning of neural cells.

Disruptions in Gbx2 function can lead to neural tube defects and hindbrain malformations. Such abnormalities may associate with disorders like Joubert Syndrome where proper brain segmentation does not occur. Additionally Gbx2's role connects it to the Shh pathway where imbalances might exacerbate conditions like medulloblastoma a type of brain cancer found in children through interactions with proteins such as OTX2.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May act as a transcription factor for cell pluripotency and differentiation in the embryo.
See full target information GBX2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 545:48-53 PubMed28445462

2017

Cell diversity and network dynamics in photosensitive human brain organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Quadrato,Tuan Nguyen,Evan Z Macosko,John L Sherwood,Sung Min Yang,Daniel R Berger,Natalie Maria,Jorg Scholvin,Melissa Goldman,Justin P Kinney,Edward S Boyden,Jeff W Lichtman,Ziv M Williams,Steven A McCarroll,Paola Arlotta
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com