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AB227853

Anti-Gbx2 antibody

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(2 Publications )

Rabbit Polyclonal GBX2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GBX2.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Gbx2 antibody (AB227853)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Gbx2 antibody (AB227853)

4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Gbx2 (green) using ab227853 at 1/500 dilution in ICC/IF.

Counterstain : Hoechst 33342 (blue), phalloidin cytoskeletal marker (red).

Western blot - Anti-Gbx2 antibody (AB227853)
  • WB

Supplier Data

Western blot - Anti-Gbx2 antibody (AB227853)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-Gbx2 antibody (ab227853) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg

Lane 3:

IMR32 whole cell extract at 30 µg

Lane 4:

SK-N-AS whole cell extract at 30 µg

Predicted band size: 37 kDa

false

Western blot - Anti-Gbx2 antibody (AB227853)
  • WB

Supplier Data

Western blot - Anti-Gbx2 antibody (AB227853)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-Gbx2 antibody (ab227853) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg

Lane 2:

C8D30 whole cell extract at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell extract at 30 µg

Predicted band size: 37 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GBX2. The exact immunogen used to generate this antibody is proprietary information.

P52951

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gbx2 also known as Gastrulation Brain Homeobox 2 functions as a transcription factor. This protein approximately 24 kDa in mass plays a significant role in gene regulation during embryonic development. Gbx2 is expressed mainly in the developing nervous system particularly in the hindbrain and midbrain regions. It influences the expression of other genes critical for brain and cranial nerve development.
Biological function summary

The role of this transcription factor centers on regulating neural development processes. Gbx2 participates in formation and patterning of the central nervous system. It does not typically operate alone; instead it often interacts with other transcription factors to modulate gene expression. Gbx2 is important for demarcating boundaries in the hindbrain which contributes to proper compartmentalization and formation of rhombomeres.

Pathways

Gbx2 contributes significantly to the Wnt and FGF signaling pathways which are fundamental during early neural development stages. In these pathways Gbx2 interacts closely with other key proteins such as Otx2 another homeobox gene to establish clear anterior-posterior axis in the developing brain. These interactions are essential to ensure proper differentiation and positioning of neural cells.

Disruptions in Gbx2 function can lead to neural tube defects and hindbrain malformations. Such abnormalities may associate with disorders like Joubert Syndrome where proper brain segmentation does not occur. Additionally Gbx2's role connects it to the Shh pathway where imbalances might exacerbate conditions like medulloblastoma a type of brain cancer found in children through interactions with proteins such as OTX2.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a transcription factor for cell pluripotency and differentiation in the embryo.
See full target information GBX2

Alternative Names

Homeobox protein GBX-2, Gastrulation and brain-specific homeobox protein 2, GBX2
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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

General physiology and biophysics 41:151-158 PubMed35416178

2022

TRAF3 plays a role in lupus nephritis by regulating Th17 cell and Treg cell balance as well as NF-κB signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Qian Sun,Wen-Si Liu,Hui-Ming Zhang,Chun-Yan Xuan,Yue Wang,Bing-Bing Fu

The journal of gene medicine 22:e3147 PubMed31758726

2019

GBX2, as a tumor promoter in lung adenocarcinoma, enhances cells viability, invasion and migration by regulating the AKT/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanchun Wang,Jianping Hui,Renting Li,Qiaoqiao Fu,Pu Yang,Yingchun Xiao,Jianrong Hui
View all publications
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Product promise

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