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AB314020

Anti-GC-C antibody [2E5] - Human IgG1 (Chimeric)

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Mouse Recombinant Monoclonal GC-C antibody. Suitable for Flow Cyt, FuncS and reacts with Human, Transfected cell line - Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GUCY2C.

View Alternative Names

GUC2C, STAR, GUCY2C, Guanylyl cyclase C, GC-C, Heat-stable enterotoxin receptor, Intestinal guanylate cyclase, STA receptor, hSTAR

3 Images
Flow Cytometry - Anti-GC-C antibody [2E5] - Human IgG1 (Chimeric) (AB314020)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-GC-C antibody [2E5] - Human IgG1 (Chimeric) (AB314020)

T84 cells were stained with human IgG (green line) and ab314020 (pink line) (2µg/1x10E6cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Flow Cytometry - Anti-GC-C antibody [2E5] - Human IgG1 (Chimeric) (AB314020)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-GC-C antibody [2E5] - Human IgG1 (Chimeric) (AB314020)

Untransfected CT26 cells (green line) and transfected human GUCY2C (GC-C) CT26 stable cells (pink line) were stained with ab314020 (2µg/1x10E6cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Functional Studies - Anti-GC-C antibody [2E5] - Human IgG1 (Chimeric) (AB314020)
  • FuncS

Supplier Data

Functional Studies - Anti-GC-C antibody [2E5] - Human IgG1 (Chimeric) (AB314020)

The binding activity of human GC-C with ab314020. Activity : Measured by its binding ability in a functional ELISA. Immobilized human GC-C at 5 μg/mL can bind ab314020, the EC50is 5.630-9.996 ng/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2E5

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

FuncS, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GUCY2C.

P25092

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/200", "FlowCyt-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FuncS-species-checked": "testedAndGuaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p></p>" }, "Transfected cell line - Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/200", "FlowCyt-species-notes": "<p></p>", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GC-C also known as guanylate cyclase C is an enzyme with mass around 120 kDa expressed mainly in the intestinal epithelium. This membrane-bound receptor plays an important role in the regulation of fluid and electrolyte balance in the intestine. GC-C gets activated by its ligands including bacterial heat-stable enterotoxins as well as endogenous peptides like guanylin and uroguanylin. Upon activation GC-C catalyzes the conversion of GTP to cGMP thereby initiating a signaling cascade that affects ion transport in intestinal cells.
Biological function summary

GC-C is part of a signaling mechanism involved in maintaining fluid balance and gut homeostasis. The enzyme participates in the cGMP-mediated signaling pathway essential for modulating chloride and bicarbonate ion secretion leading to water loss into the intestinal lumen. GC-C does not work in isolation but cooperates with other molecules to facilitate these functions. Specifically it associates with ion transporters and channels influencing the enteric nervous system.

Pathways

GC-C functions within the cGMP pathway which is critical for intestinal fluid regulation. GC-C also connects with the Wnt signaling pathway an important metabolic relay influencing cell proliferation and differentiation. By producing cGMP GC-C influences down-stream proteins such as protein kinase G (PKG) and cystic fibrosis transmembrane conductance regulator (CFTR). These proteins play pivotal roles in maintaining the physiological processes in the intestine.

GC-C relates to irritable bowel syndrome (IBS) and colorectal cancer. Overactivation of GC-C by bacterial toxins can lead to enterotoxigenic diarrhea common in IBS. Its role in colorectal cancer arises from dysregulated signaling pathways affecting cellular growth. Mutations and alterations in GC-C expression may contribute to cancer progression. Furthermore GC-C-related dysregulation can involve other proteins like E-cadherin which modulates cell adhesion in tumor pathways complicating disease dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanylyl cyclase that catalyzes synthesis of cyclic GMP (cGMP) from GTP (PubMed : 11950846, PubMed : 1718270, PubMed : 22436048, PubMed : 22521417, PubMed : 23269669). Receptor for the E.coli heat-stable enterotoxin; E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GUCY2C (PubMed : 1680854, PubMed : 1718270). Also activated by the endogenous peptides guanylin and uroguanylin (PubMed : 8381596).
See full target information GUCY2C
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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