AB225864

Anti-GC-C antibody [EPR22056-278]

Name: Anti-GC-C antibody [EPR22056-278] (ab225864) | Abcam
Brand: Abcam Limited
SKU: AB225864
Price: 215 USD
Availability: InStock
Rating: 5 (1 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal GC-C antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

Guc2c, Gucy2c, Guanylyl cyclase C, GC-C, Heat-stable enterotoxin receptor, Intestinal guanylate cyclase, STA receptor

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelium of rat colon (PMID : 27246004) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelium of mouse colon (PMID : 27246004) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.

Negative control : no staining on mouse spleen (PMID : 26986842) is observed.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-GC-C antibody [EPR22056-278] (AB225864)

Blocking/Dilution buffer : 5% NFDM/TBST.

GC-C migrates as two differentially glycosylated proteins with molecular masses of 130 and 145 kDa (PMID : 24217248).

All lanes:

Western blot - Anti-GC-C antibody [EPR22056-278] (ab225864) at 1/1000 dilution

All lanes:

Rat colon lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 123 kDa

Observed band size: 130 kDa,145 kDa

false

Exposure time: 37s

Supplier Data

Western blot - Anti-GC-C antibody [EPR22056-278] (AB225864)

Exposure time : Lane 1 : 3 minutes; Lane 2 : 26 seconds.

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

GC-C migrates as a glycosylated 145 kDa protein and a 90 kDa cleavage product (PMID : 9344852; PMID : 26986842).

Negative control : mouse spleen.

All lanes:

Western blot - Anti-GC-C antibody [EPR22056-278] (ab225864) at 1/1000 dilution

Lane 1:

Mouse spleen lysate at 10 µg

Lane 2:

Mouse colon lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 123 kDa

Observed band size: 145 kDa,90 kDa

false

Carrier free

Anti-GC-C antibody [EPR22056-278] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22056-278

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GC-C also known as guanylate cyclase C is an enzyme with mass around 120 kDa expressed mainly in the intestinal epithelium. This membrane-bound receptor plays an important role in the regulation of fluid and electrolyte balance in the intestine. GC-C gets activated by its ligands including bacterial heat-stable enterotoxins as well as endogenous peptides like guanylin and uroguanylin. Upon activation GC-C catalyzes the conversion of GTP to cGMP thereby initiating a signaling cascade that affects ion transport in intestinal cells.

Biological function summary

GC-C is part of a signaling mechanism involved in maintaining fluid balance and gut homeostasis. The enzyme participates in the cGMP-mediated signaling pathway essential for modulating chloride and bicarbonate ion secretion leading to water loss into the intestinal lumen. GC-C does not work in isolation but cooperates with other molecules to facilitate these functions. Specifically it associates with ion transporters and channels influencing the enteric nervous system.

Pathways

GC-C functions within the cGMP pathway which is critical for intestinal fluid regulation. GC-C also connects with the Wnt signaling pathway an important metabolic relay influencing cell proliferation and differentiation. By producing cGMP GC-C influences down-stream proteins such as protein kinase G (PKG) and cystic fibrosis transmembrane conductance regulator (CFTR). These proteins play pivotal roles in maintaining the physiological processes in the intestine.

GC-C relates to irritable bowel syndrome (IBS) and colorectal cancer. Overactivation of GC-C by bacterial toxins can lead to enterotoxigenic diarrhea common in IBS. Its role in colorectal cancer arises from dysregulated signaling pathways affecting cellular growth. Mutations and alterations in GC-C expression may contribute to cancer progression. Furthermore GC-C-related dysregulation can involve other proteins like E-cadherin which modulates cell adhesion in tumor pathways complicating disease dynamics.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Guanylyl cyclase that catalyzes synthesis of cyclic GMP (cGMP) from GTP.

See full target information Gucy2c

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1624 PubMed40855239

2025

DNA methylation regulates TREM1 expression to modulate immune responses and drive progression in colorectal neuroendocrine neoplasm as a potential therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Guo,Guiwen Zheng,Jia Li,Shuzhan Yao,Qiang Jia,Jian Tan,Zhaowei Meng

Nan fang yi ke da xue xue bao = Journal of Southern Medical University 41:376-383 PubMed33849828

2021

[Changes of guanylate cyclase C in colon tissues of rats with intestinal injury associated with severe acute pancreatitis].

Applications

Unspecified application

Species

Unspecified reactive species

B Wang,Q Huang,S Li,J Wu,X Yuan,H Sun,L Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GC-C antibody [EPR22056-278]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (AB225864)

Western blot - Anti-GC-C antibody [EPR22056-278] (AB225864)

Western blot - Anti-GC-C antibody [EPR22056-278] (AB225864)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise