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Rabbit Recombinant Monoclonal GC1q R antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Mouse
Tested
Not recommended
Not recommended
Not recommended
Tested
Rat
Tested
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Target data

Function

Is believed to be a multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, protein synthesis in mitochondria, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. At the cell surface is thought to act as an endothelial receptor for plasma proteins of the complement and kallikrein-kinin cascades. Putative receptor for C1q; specifically binds to the globular 'heads' of C1q thus inhibiting C1; may perform the receptor function through a complex with C1qR/CD93. In complex with cytokeratin-1/KRT1 is a high affinity receptor for kininogen-1/HMWK. Can also bind other plasma proteins, such as coagulation factor XII leading to its autoactivation. May function to bind initially fluid kininogen-1 to the cell membrane. The secreted form may enhance both extrinsic and intrinsic coagulation pathways. It is postulated that the cell surface form requires docking with transmembrane proteins for downstream signaling which might be specific for a cell-type or response. By acting as C1q receptor is involved in chemotaxis of immature dendritic cells and neutrophils and is proposed to signal through CD209/DC-SIGN on immature dendritic cells, through integrin alpha-4/beta-1 during trophoblast invasion of the decidua, and through integrin beta-1 during endothelial cell adhesion and spreading. Signaling involved in inhibition of innate immune response is implicating the PI3K-AKT/PKB pathway. Required for protein synthesis in mitochondria (PubMed:28942965). In mitochondrial translation may be involved in formation of functional 55S mitoribosomes; the function seems to involve its RNA-binding activity. May be involved in the nucleolar ribosome maturation process; the function may involve the exchange of FBL for RRP1 in the association with pre-ribosome particles. Involved in regulation of RNA splicing by inhibiting the RNA-binding capacity of SRSF1 and its phosphorylation. Is required for the nuclear translocation of splicing factor U2AF1L4. Involved in regulation of CDKN2A- and HRK-mediated apoptosis. Stabilizes mitochondrial CDKN2A isoform smARF. May be involved in regulation of FOXC1 transcriptional activity and NFY/CCAAT-binding factor complex-mediated transcription. May play a role in antibacterial defense as it can bind to cell surface hyaluronan and inhibit Streptococcus pneumoniae hyaluronate lyase. May be involved in modulation of the immune response; ligation by HCV core protein is resulting in suppression of interleukin-12 production in monocyte-derived dendritic cells. Involved in regulation of antiviral response by inhibiting DDX58- and IFIH1-mediated signaling pathways probably involving its association with MAVS after viral infection.(Microbial infection) Involved in HIV-1 replication, presumably by contributing to splicing of viral RNA.(Microbial infection) In infection processes acts as an attachment site for microbial proteins, including Listeria monocytogenes internalin B (InlB) and Staphylococcus aureus protein A.(Microbial infection) Involved in replication of Rubella virus.

Alternative names

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Rabbit Recombinant Monoclonal GC1q R antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR8871

Purification technique

Affinity purification Protein A

Dissociation constant

4 x 10-12 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

GC1qR takes part in complement activation and immune surveillance by interacting with the complement C1 complex and various plasma proteins. As a component of high-molecular-weight complexes it modulates important cellular processes like inflammation apoptosis and infection regulation. Its presence on the mitochondrial membrane suggests a role in maintaining mitochondrial dynamics and function under stress conditions. By engaging in these arenas gC1qR serves as an intermediary in cellular signaling and immune response.

Activity summary

The gC1qR also known as gC1q-binding protein or C1qbp is a multifunctional protein involved in various cellular processes. It has an approximate mass of 33 kDa and is widely expressed on the cell surface mitochondria and in the cytoplasm of different cell types. gC1qR interacts with several ligands including C1q involved in the classical complement pathway. Its versatility in binding interactions allows it to influence several cellular environments and functions.

Pathways

GC1qR is significant in the classical complement pathway and apoptotic signaling pathways. It interacts with proteins like C1q and thrombomodulin influencing complement activation and coagulation pathways. It contributes to the propagation of immune responses by enhancing the interface between innate and adaptive immunity. Its interactions within these pathways highlight its role in maintaining cellular homeostasis and responding to environmental challenges.

Associated diseases and disorders

GC1qR associates closely with systemic lupus erythematosus and cancer. Dysregulation in its expression or function can lead to aberrant immune responses and contribute to autoimmune diseases. In the context of cancer gC1qR may interact with proteins such as HMGB1 and annexin A2 affecting tumor progression and metastasis. Understanding its role in these disorders aids in exploring potential therapeutic strategies targeting gC1qR interactions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-GC1q R antibody [EPR8871] (ab131284), expandable thumbnail

    Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-GC1q R antibody [EPR8871] (AB131284) at 0.01 µg/mL

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: Human heart lysates at 15 µg

    Lane 3: Mouse heart lysates at 15 µg

    Lane 4: Rat heart lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 31 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling GC1q R with Purified ab131284 at 1:250 dilution (0.43 μg/ml). Heat mediated antigen retrieval was performed using Citrate buffer (pH 6.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

  • Western blot - Anti-GC1q R antibody [EPR8871] (ab131284), expandable thumbnail

    Western blot - Anti-GC1q R antibody [EPR8871] (ab131284)

    All lanes: Western blot - Anti-GC1q R antibody [EPR8871] (AB131284) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: Raji cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 31 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labelled with unpurified ab131284 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR8871] (ab131284)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelled with unpurified ab131284 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-GC1q R antibody [EPR8871] (ab131284), expandable thumbnail

    OI-RD Scanning - Anti-GC1q R antibody [EPR8871] (ab131284)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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