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AB184958

Anti-GCAP2 antibody [EPR14571]

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Rabbit Recombinant Monoclonal GCAP2 antibody. Suitable for WB and reacts with Mouse, Rat samples.

View Alternative Names

GCAP2, GUCA1B, Guanylyl cyclase-activating protein 2, GCAP 2, Guanylate cyclase activator 1B

2 Images
Western blot - Anti-GCAP2 antibody [EPR14571] (AB184958)
  • WB

Supplier Data

Western blot - Anti-GCAP2 antibody [EPR14571] (AB184958)

All lanes:

Western blot - Anti-GCAP2 antibody [EPR14571] (ab184958) at 1/2000 dilution

All lanes:

Rat eyeball lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

Western blot - Anti-GCAP2 antibody [EPR14571] (AB184958)
  • WB

Supplier Data

Western blot - Anti-GCAP2 antibody [EPR14571] (AB184958)

All lanes:

Western blot - Anti-GCAP2 antibody [EPR14571] (ab184958) at 1/1000 dilution

All lanes:

Mouse eyeball lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

  • Carrier free

    Anti-GCAP2 antibody [EPR14571] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14571

Isotype

IgG

Carrier free

No

Reacts with

Rat, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Guanylyl cyclase-activating protein 2 (GCAP2) also known as GUCA1B is a calcium-binding protein with a molecular weight of approximately 24 kDa. GCAP2 plays an important role in the visual system's phototransduction process. It is primarily expressed in the retina especially in photoreceptor cells. GCAP2 is part of a family of neuronal calcium sensor proteins that adjust their activities based on calcium concentration changes. These adjustments help regulate the sensitivity of photoreceptors to light.
Biological function summary

This protein modulates the activity of retinal guanylyl cyclases. In photoreceptors it forms complexes with these enzymes to regulate the synthesis of cyclic GMP in response to light. The availability of cyclic GMP is essential for the recovery phase of phototransduction which allows visual cells to respond to light changes efficiently. GCAP2 switching between active and inactive states adjusts the production of cyclic GMP depending on calcium levels inside the cells.

Pathways

GCAP2 features in the phototransduction cascade an essential pathway for converting light into visual signals. This cascade involves several proteins including rhodopsin transducin and phosphodiesterase. GCAP2’s role focuses on regulating guanylyl cyclases allowing the restoration of cyclic GMP levels which is important for returning ion channels to their open state after photon absorption. It is tightly connected to GCAP1 another guanylyl cyclase-activating protein with similar functions but they operate under slightly different calcium concentrations.

GCAP2 mutations have been linked to certain forms of cone and cone-rod dystrophies retinal degenerative disorders that affect vision. Changes in GCAP2 function influence cyclic GMP levels disrupting normal photoreceptor activity and survival. Mutations can interact with the rhodopsin promoter influencing disease progression and severity. Understanding GCAP2’s role provides potential targets for treating these retinal disorders and improving photoreceptor health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stimulates two retinal guanylyl cyclases (GCs) GUCY2D and GUCY2F when free calcium ions concentration is low, and inhibits GUCY2D and GUCY2F when free calcium ions concentration is elevated (By similarity). This Ca(2+)-sensitive regulation of GCs is a key event in recovery of the dark state of rod photoreceptors following light exposure (By similarity). May be involved in cone photoreceptor response and recovery of response in bright light (By similarity).
See full target information GUCA1B
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Product promise

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